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950  structures 2932  species 22  interactions 37684  sequences 1623  architectures

Clan: HotDog (CL0050)


HotDog superfamily Add an annotation

The HotDog fold was first observed in the structure of Escherichia coli beta-hydroxydecanoyl thiol ester dehydratase (FabA), where Leesong et al. noticed that each subunit of this dimeric enzyme contained a mixed alpha + beta 'hot dog' fold. They described the seven-stranded antiparallel beta-sheet as the 'bun', which wraps around a five-turn alpha-helical 'sausage', This superfamily contains a diverse range of enzymes. Membership includes numerous prokaryotic, archaeal and eukaryotic proteins involved in several related, but distinct, catalytic activities, from metabolic roles such as thioester hydrolysis in fatty acid metabolism, to degradation of phenylacetic acid and the environmental pollutant 4-chlorobenzoate. The superfamily also includes FapR, a non-catalytic bacterial homologue that is involved in transcriptional regulation of fatty acid biosynthesis [1].

This clan contains 13 families and the total number of domains in the clan is 37684. The clan was built by A Bateman.

Literature references

  1. Dillon SC, Bateman A; , BMC Bioinformatics 2004;5:109-109.: The Hotdog fold: wrapping up a superfamily of thioesterases and dehydratases. PUBMED:15307895 EPMC:15307895


This clan contains the following 13 member families:

4HBT 4HBT_2 4HBT_3 Acyl-ACP_TE Acyl_CoA_thio AfsA DUF4442 FabA FcoT MaoC_dehydrat_N MaoC_dehydratas PS-DH YiiD_C

External database links

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
4HBT (PF03061) 12476 (33.1%) View
MaoC_dehydratas (PF01575) 5838 (15.5%) View
PS-DH (PF14765) 5673 (15.1%) View
FabA (PF07977) 3198 (8.5%) View
4HBT_2 (PF13279) 3105 (8.2%) View
4HBT_3 (PF13622) 2867 (7.6%) View
MaoC_dehydrat_N (PF13452) 1965 (5.2%) View
Acyl-ACP_TE (PF01643) 1222 (3.2%) View
DUF4442 (PF14539) 679 (1.8%) View
AfsA (PF03756) 349 (0.9%) View
YiiD_C (PF09500) 232 (0.6%) View
Acyl_CoA_thio (PF02551) 50 (0.1%) View
FcoT (PF10862) 30 (0.1%) View
Total: 13 Total: 37684 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

Tree controls


This tree shows the occurrence of the domains in this clan across different species. More...



There are 22 interactions for this clan. More...

Interacting families
4HBT_2 4HBT_2
4HBT_3 4HBT_3
Acyl_CoA_thio Acyl_CoA_thio
ADH_zinc_N PS-DH
DUF4442 DUF4442
ketoacyl-synt FabA
MaoC_dehydrat_N Acyl_transf_1
MaoC_dehydratas Acyl_transf_1
PS-DH ADH_zinc_N
YiiD_C YiiD_C


For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

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