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780  structures 6664  species 12  interactions 120193  sequences 821  architectures

Clan: HAD (CL0137)


HAD superfamily Add an annotation

This clan represents the haloacid dehalogenase (HAD) superfamily that includes a diverse range of enzymes that use an asp carboxylate as a nucleophile [1].

This clan contains 21 families and the total number of domains in the clan is 120193. The clan was built by A Bateman.

Literature references

  1. Koonin EV, Tatusov RL; , J Mol Biol 1994;244:125-132.: Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search. PUBMED:7966317 EPMC:7966317
  2. Aravind L, Galperin MY, Koonin EV; , Trends Biochem Sci 1998;23:127-129.: The catalytic domain of the P-type ATPase has the haloacid dehalogenase fold. PUBMED:9584613 EPMC:9584613


This clan contains the following 21 member families:

5_nucleotid Acid_phosphat_B Acid_PPase DUF705 HAD HAD_2 Hydrolase Hydrolase_3 Hydrolase_6 Hydrolase_like Hydrolase_like2 LNS2 NIF NT5C PGP_phosphatase PMM PNK3P Put_Phosphatase S6PP Trehalose_PPase UMPH-1

External database links

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
HAD_2 (PF13419) 33740 (28.1%) View
Hydrolase (PF00702) 27368 (22.8%) View
Hydrolase_3 (PF08282) 20961 (17.4%) View
HAD (PF12710) 9618 (8.0%) View
Hydrolase_like (PF13242) 8522 (7.1%) View
Hydrolase_6 (PF13344) 4798 (4.0%) View
NIF (PF03031) 2877 (2.4%) View
Trehalose_PPase (PF02358) 2499 (2.1%) View
Acid_phosphat_B (PF03767) 1773 (1.5%) View
Hydrolase_like2 (PF13246) 1746 (1.5%) View
PNK3P (PF08645) 1136 (0.9%) View
Pfam family Num. domains Alignment
S6PP (PF05116) 845 (0.7%) View
LNS2 (PF08235) 814 (0.7%) View
NT5C (PF06941) 770 (0.6%) View
PMM (PF03332) 691 (0.6%) View
5_nucleotid (PF05761) 680 (0.6%) View
Put_Phosphatase (PF06888) 337 (0.3%) View
Acid_PPase (PF12689) 326 (0.3%) View
PGP_phosphatase (PF09419) 326 (0.3%) View
UMPH-1 (PF05822) 291 (0.2%) View
DUF705 (PF05152) 75 (0.1%) View
Total: 21 Total: 120193 Clan alignment

Please note: Clan alignments can be very large and can cause problems for some browsers. Read the note above before viewing.

Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

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This tree shows the occurrence of the domains in this clan across different species. More...



There are 12 interactions for this clan. More...

Interacting families
5_nucleotid 5_nucleotid
Hydrolase E1-E2_ATPase
Acid_phosphat_B Acid_phosphat_B
Hydrolase_3 Hydrolase_3


For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

Loading structure mapping...