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604  structures 5270  species 23  interactions 25264  sequences 179  architectures

Clan: GCS (CL0286)

Summary

gamma-glutamylcysteine synthetase/glutamine synthetase clan Add an annotation

This clan represents a superfamily of carboxylate-amine/ammonia ligases [1] that includes Gamma-Glutamylcysteine synthetase (gamma-GCS) and glutamine synthetase (GS). Gamma-Glutamylcysteine synthetase (gamma-GCS) catalyses the first step in the de novo biosynthesis of glutathione.

This clan contains 9 families and the total number of domains in the clan is 25264. The clan was built by A Bateman and J Pei.

Literature references

  1. Abbott JJ, Pei J, Ford JL, Qi Y, Grishin VN, Pitcher LA, Phillips MA, Grishin NV; , J Biol Chem. 2001;276:42099-42107.: Structure prediction and active site analysis of the metal binding determinants in gamma -glutamylcysteine synthetase. PUBMED:11527962 EPMC:11527962

Members

This clan contains the following 9 member families:

Amidoligase_2 ATP-gua_Ptrans DUF2126 GatB_N GCS GCS2 Gln-synt_C Glu_cys_ligase Pup_ligase

External database links

Domain organisation

Below is a listing of the unique domain organisations or architectures from this clan. More...

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Alignments

The table below shows the number of occurrences of each domain throughout the sequence database. More...

Pfam family Num. domains Alignment
Gln-synt_C (PF00120) 11153 (44.1%) View
GatB_N (PF02934) 4869 (19.3%) View
GCS2 (PF04107) 2905 (11.5%) View
ATP-gua_Ptrans (PF00217) 1638 (6.5%) View
Glu_cys_ligase (PF04262) 1115 (4.4%) View
Pup_ligase (PF03136) 1073 (4.2%) View
GCS (PF03074) 963 (3.8%) View
Amidoligase_2 (PF12224) 931 (3.7%) View
DUF2126 (PF09899) 617 (2.4%) View
Total: 9 Total: 25264 Clan alignment
 

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Family relationships

This diagram shows the relationships between members of this clan. More...

Species distribution

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This tree shows the occurrence of the domains in this clan across different species. More...

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Interactions

There are 23 interactions for this clan. More...

Interacting families
A B
Amidase GatB_N
Asparaginase GatB_N
ATP-grasp_4 Glu_cys_ligase
ATP-gua_Ptrans ATP-gua_Ptrans
ATP-gua_PtransN
ATP-gua_PtransN ATP-gua_Ptrans
GatB_N Amidase
GAD
GatB_N
GatB_Yqey
Glu-tRNAGln
tRNA-synt_1c
GCS2 GCS2
Gln-synt_C Gln-synt_C
Gln-synt_N
GSIII_N
Gln-synt_N Gln-synt_C
Glu-tRNAGln GatB_N
Glu_cys_ligase ATP-grasp_4
Glu_cys_ligase
GSIII_N Gln-synt_C
Pup_ligase Pup
tRNA-synt_2 GatB_N

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the MSD group, to allow us to map Pfam domains onto UniProt three-dimensional structures. The table below shows the mapping between the Pfam families in this clan, the corresponding UniProt entries, and the region of the three-dimensional structures that are available for that sequence.

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