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0  structures 69  species 0  interactions 164  sequences 7  architectures

Family: Bul1_N (PF04425)

Summary: Bul1 N terminus

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Bul1 N terminus Provide feedback

This family contains the N terminus of Saccharomyces cerevisiae Bul1. Bul1 binds the ubiquitin ligase Rsp5, via an N terminal PPSY motif (157-160 in P48524) [1]. The complex containing Bul1 and Rsp5 is involved in intracellular trafficking of the general amino acid permease Gap1 [2] degradation of Rog1 in cooperation with Bul2 and GSK-3 [3] and mitochondrial inheritance [4]. Bul1 may contain HEAT repeats.

Literature references

  1. Yashiroda H, Kaida D, Toh-e A, Kikuchi Y; , Gene 1998;225:39-46.: The PY-motif of Bul1 protein is essential for growth of Saccharomyces cerevisiae under various stress conditions. PUBMED:9931424 EPMC:9931424

  2. Soetens O, De Craene JO, Andre B; , J Biol Chem 2001;276:43949-43957.: Ubiquitin is required for sorting to the vacuole of the yeast general amino acid permease, Gap1. PUBMED:11500494 EPMC:11500494

  3. Andoh T, Hirata Y, Kikuchi A; , Mol Cell Biol 2000;20:6712-6720.: Yeast glycogen synthase kinase 3 is involved in protein degradation in cooperation with Bul1, Bul2, and Rsp5. PUBMED:10958669 EPMC:10958669

  4. Fisk HA, Yaffe MP; , J Cell Biol 1999;145:1199-1208.: A role for ubiquitination in mitochondrial inheritance in Saccharomyces cerevisiae. PUBMED:10366593 EPMC:10366593


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR007519

This domain is the N terminus of Saccharomyces cerevisiae (Baker's yeast) Bul1. Bul1 binds the ubiquitin ligase Rsp5, via an N-terminal PPSY motif (157-160 in SWISSPROT) [PUBMED:9931424]. The complex containing Bul1 and Rsp5 is involved in intracellular trafficking of the general amino acid permease Gap1 [PUBMED:11500494], degradation of Rog1 in cooperation with Bul2 and GSK-3 [PUBMED:10958669], and mitochondrial inheritance [PUBMED:10366593]. Bul1 may contain HEAT repeats. The C terminus is INTERPRO.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Arrestin_N-like (CL0135), which has the following description:

The families in this clan are involved in vacuolar protein trafficking, G protein signal termination and sporulation. The Arrestin N terminal domain has an Ig-like beta sandwich fold which binds to receptors and impairs their capacity to active G proteins [1]. Arrestins have also been implicated in the endocytosis of receptors and cross talk with other signalling pathways [2].

The clan contains the following 6 members:

Arrestin_C Arrestin_N Bul1_N LDB19 Spo0M Vps26

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(27)
Full
(164)
Representative proteomes NCBI
(170)
Meta
(0)
RP15
(10)
RP35
(51)
RP55
(98)
RP75
(105)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(27)
Full
(164)
Representative proteomes NCBI
(170)
Meta
(0)
RP15
(10)
RP35
(51)
RP55
(98)
RP75
(105)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(27)
Full
(164)
Representative proteomes NCBI
(170)
Meta
(0)
RP15
(10)
RP35
(51)
RP55
(98)
RP75
(105)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: DOMO:DM04045;
Previous IDs: none
Type: Family
Author: Kerrison ND
Number in seed: 27
Number in full: 164
Average length of the domain: 361.50 aa
Average identity of full alignment: 28 %
Average coverage of the sequence by the domain: 49.70 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 28.7 28.7
Trusted cut-off 29.0 28.7
Noise cut-off 28.4 28.4
Model length: 440
Family (HMM) version: 7
Download: download the raw HMM for this family

Species distribution

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