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254  structures 1596  species 0  interactions 21034  sequences 986  architectures

Family: EF-hand_8 (PF13833)

Summary: EF-hand domain pair

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "EF hand". More...

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

EF-hand domain pair Provide feedback

No Pfam abstract.

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR002048

Many calcium-binding proteins belong to the same evolutionary family and share a type of calcium-binding domain known as the EF-hand. This type of domain consists of a twelve residue loop flanked on both sides by a twelve residue alpha-helical domain. In an EF-hand loop the calcium ion is coordinated in a pentagonal bipyramidal configuration. The six residues involved in the binding are in positions 1, 3, 5, 7, 9 and 12; these residues are denoted by X, Y, Z, -Y, -X and -Z. The invariant Glu or Asp at position 12 provides two oxygens for liganding Ca (bidentate ligand). Ca2 binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers [ PUBMED:9228939 , PUBMED:8848832 , PUBMED:7553064 , PUBMED:7656053 , PUBMED:10591109 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(34)
Full
(21034)
Representative proteomes UniProt
(50165)
RP15
(3832)
RP35
(9220)
RP55
(17712)
RP75
(23634)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(34)
Full
(21034)
Representative proteomes UniProt
(50165)
RP15
(3832)
RP35
(9220)
RP55
(17712)
RP75
(23634)
Alignment:
Format:
Order:
Sequence:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(34)
Full
(21034)
Representative proteomes UniProt
(50165)
RP15
(3832)
RP35
(9220)
RP55
(17712)
RP75
(23634)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Jackhmmer:B4DPI1
Previous IDs: EF_hand_6;
Type: Domain
Sequence Ontology: SO:0000417
Author: Coggill P
Number in seed: 34
Number in full: 21034
Average length of the domain: 49.20 aa
Average identity of full alignment: 22 %
Average coverage of the sequence by the domain: 13.61 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 28.0 13.8
Trusted cut-off 28.0 13.8
Noise cut-off 27.9 13.7
Model length: 54
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the EF-hand_8 domain has been found. There are 254 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0B4KHF1 View 3D Structure Click here
A0A0G2K2W5 View 3D Structure Click here
A0A0G2LAC3 View 3D Structure Click here
A0A0N7KEZ6 View 3D Structure Click here
A0A0P0VG45 View 3D Structure Click here
A0A0P0VH48 View 3D Structure Click here
A0A0P0W7C9 View 3D Structure Click here
A0A0P0WI02 View 3D Structure Click here
A0A0R0EIV3 View 3D Structure Click here
A0A0R0EXE0 View 3D Structure Click here
A0A0R0FB11 View 3D Structure Click here
A0A0R0FB11 View 3D Structure Click here
A0A0R0G3H9 View 3D Structure Click here
A0A0R0GP97 View 3D Structure Click here
A0A0R0INJ2 View 3D Structure Click here
A0A0R0JLM2 View 3D Structure Click here
A0A0R4IPX8 View 3D Structure Click here
A0A1D6E6R4 View 3D Structure Click here
A0A1D6FBD8 View 3D Structure Click here
A0A1D6FSL1 View 3D Structure Click here
A0A1D6GU50 View 3D Structure Click here
A0A1D6HD01 View 3D Structure Click here
A0A1D6INZ6 View 3D Structure Click here
A0A1D6J9N5 View 3D Structure Click here
A0A1D6K120 View 3D Structure Click here
A0A1D6K352 View 3D Structure Click here
A0A1D6KIL0 View 3D Structure Click here
A0A1D6KXI8 View 3D Structure Click here
A0A1D6L3Q6 View 3D Structure Click here
A0A1D6L8S9 View 3D Structure Click here
A0A1D6LN51 View 3D Structure Click here
A0A1D6M749 View 3D Structure Click here
A0A1D6MQ76 View 3D Structure Click here
A0A1D6MXK9 View 3D Structure Click here
A0A1D6N0T4 View 3D Structure Click here
A0A1D6N0T4 View 3D Structure Click here
A0A1D6N9T0 View 3D Structure Click here
A0A1D6NAQ2 View 3D Structure Click here
A0A1D6NLC2 View 3D Structure Click here
A0A1D6P328 View 3D Structure Click here
A0A1D6PBR7 View 3D Structure Click here
A0A1D6QA01 View 3D Structure Click here
A0A1D6QH05 View 3D Structure Click here
A0A1D8PEF8 View 3D Structure Click here
A0A2R8PV64 View 3D Structure Click here
A0A2R8Q430 View 3D Structure Click here
A0A2R8Q8P9 View 3D Structure Click here
A0A2R8Q964 View 3D Structure Click here
A0A2R8QDC7 View 3D Structure Click here
A0A2R8QHG5 View 3D Structure Click here
A0A2R8QMM6 View 3D Structure Click here
A0A2R8QQY2 View 3D Structure Click here
A0A2R8RIQ9 View 3D Structure Click here
A0A2R8RNP1 View 3D Structure Click here
A2ASZ8 View 3D Structure Click here
A2VEI2 View 3D Structure Click here
A4GNA8 View 3D Structure Click here
A4HT58 View 3D Structure Click here
A4HVP4 View 3D Structure Click here
A4HZG8 View 3D Structure Click here
A4I005 View 3D Structure Click here
A4I967 View 3D Structure Click here
A4IA71 View 3D Structure Click here
A4IG32 View 3D Structure Click here
A8Y589 View 3D Structure Click here
A9JTH1 View 3D Structure Click here
B0BNJ1 View 3D Structure Click here
B6SKK9 View 3D Structure Click here
B6SL16 View 3D Structure Click here
B6SLW1 View 3D Structure Click here
B6SRA7 View 3D Structure Click here
B6T0B8 View 3D Structure Click here
B6T8N9 View 3D Structure Click here
B6TXR3 View 3D Structure Click here
B6U0W1 View 3D Structure Click here
B6U492 View 3D Structure Click here
B8A6G0 View 3D Structure Click here
B8JK25 View 3D Structure Click here
B8JK25 View 3D Structure Click here
C0PEX3 View 3D Structure Click here
C6SZ72 View 3D Structure Click here
C6T1U2 View 3D Structure Click here
C6T335 View 3D Structure Click here
C6TG22 View 3D Structure Click here
C6TM17 View 3D Structure Click here
D3ZG95 View 3D Structure Click here
D3ZII9 View 3D Structure Click here
D3ZYI0 View 3D Structure Click here
D4A938 View 3D Structure Click here
E7FBI7 View 3D Structure Click here
E7FF65 View 3D Structure Click here
E7FFY6 View 3D Structure Click here
E9QE31 View 3D Structure Click here
E9QFN0 View 3D Structure Click here
F1LQY6 View 3D Structure Click here
F1LQY6 View 3D Structure Click here
F1Q7B2 View 3D Structure Click here
F1QHQ8 View 3D Structure Click here
F1QI24 View 3D Structure Click here
F1QQI5 View 3D Structure Click here
F1QR22 View 3D Structure Click here
F1QVF2 View 3D Structure Click here
F1QYK9 View 3D Structure Click here
F1R110 View 3D Structure Click here
F1R4I7 View 3D Structure Click here
F1R647 View 3D Structure Click here
F4KAK5 View 3D Structure Click here
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I1JCT4 View 3D Structure Click here
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I1JS16 View 3D Structure Click here
I1JW47 View 3D Structure Click here
I1KAS3 View 3D Structure Click here
I1KD57 View 3D Structure Click here
I1KE75 View 3D Structure Click here
I1KN20 View 3D Structure Click here
I1KP16 View 3D Structure Click here
I1L2G2 View 3D Structure Click here
I1LJR3 View 3D Structure Click here
I1LLG4 View 3D Structure Click here
I1LQF3 View 3D Structure Click here
I1LRV1 View 3D Structure Click here
I1LU99 View 3D Structure Click here
I1LUU8 View 3D Structure Click here
I1M2E4 View 3D Structure Click here
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I1M3Z8 View 3D Structure Click here
I1M9Z0 View 3D Structure Click here
I1MAC0 View 3D Structure Click here
I1ME81 View 3D Structure Click here
I1MLK5 View 3D Structure Click here
I1MNI9 View 3D Structure Click here
I1MRF9 View 3D Structure Click here
I1N0S3 View 3D Structure Click here
I1NCN9 View 3D Structure Click here
I1NF42 View 3D Structure Click here
I6L9G5 View 3D Structure Click here
K7KCA7 View 3D Structure Click here
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K7KLX6 View 3D Structure Click here
K7KRB9 View 3D Structure Click here
K7KTG4 View 3D Structure Click here
K7KTG4 View 3D Structure Click here
K7L409 View 3D Structure Click here
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K7LIL0 View 3D Structure Click here
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K7M068 View 3D Structure Click here
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M0RCJ6 View 3D Structure Click here
O08529 View 3D Structure Click here
O14830 View 3D Structure Click here
O35350 View 3D Structure Click here
O35783 View 3D Structure Click here
O35887 View 3D Structure Click here
O43852 View 3D Structure Click here
O81831 View 3D Structure Click here
O88456 View 3D Structure Click here
P07384 View 3D Structure Click here
P16259 View 3D Structure Click here
P17655 View 3D Structure Click here
P19123 View 3D Structure Click here
P24844 View 3D Structure Click here
P28470 View 3D Structure Click here
P30626 View 3D Structure Click here
P34057 View 3D Structure Click here
P34368 View 3D Structure Click here
P35243 View 3D Structure Click here
P37235 View 3D Structure Click here
P42325 View 3D Structure Click here
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P53014 View 3D Structure Click here
P61601 View 3D Structure Click here
P62748 View 3D Structure Click here
P62749 View 3D Structure Click here
P63316 View 3D Structure Click here
P84074 View 3D Structure Click here
P84075 View 3D Structure Click here
P97571 View 3D Structure Click here
Q07009 View 3D Structure Click here
Q0DZK7 View 3D Structure Click here
Q0DZK7 View 3D Structure Click here
Q0JBY6 View 3D Structure Click here
Q0KHU5 View 3D Structure Click here
Q10MF3 View 3D Structure Click here
Q14651 View 3D Structure Click here
Q1LUD3 View 3D Structure Click here
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Q1LYC5 View 3D Structure Click here
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Q21153 View 3D Structure Click here
Q2QYW1 View 3D Structure Click here
Q3HRN9 View 3D Structure Click here
Q3HRP0 View 3D Structure Click here
Q3HRP1 View 3D Structure Click here
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Q3TFD2 View 3D Structure Click here
Q4CWI2 View 3D Structure Click here
Q4CWP2 View 3D Structure Click here
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Q4CXE6 View 3D Structure Click here
Q4D6V6 View 3D Structure Click here
Q4DDH8 View 3D Structure Click here
Q4DFL2 View 3D Structure Click here
Q4DL22 View 3D Structure Click here
Q4DLT9 View 3D Structure Click here
Q4DQ24 View 3D Structure Click here
Q4DQ44 View 3D Structure Click here
Q4DSH4 View 3D Structure Click here
Q4DU55 View 3D Structure Click here
Q4DU55 View 3D Structure Click here
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Q4PP99 View 3D Structure Click here
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Q54HB8 View 3D Structure Click here
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Q54X77 View 3D Structure Click here
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Q61112 View 3D Structure Click here
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Q6DN52 View 3D Structure Click here
Q6IQ64 View 3D Structure Click here
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Q6P069 View 3D Structure Click here
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Q6PC38 View 3D Structure Click here
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Q6PIL6 View 3D Structure Click here
Q6Z727 View 3D Structure Click here
Q6ZM98 View 3D Structure Click here
Q75GE8 View 3D Structure Click here
Q75GE8 View 3D Structure Click here
Q75K28 View 3D Structure Click here
Q75LU8 View 3D Structure Click here
Q7K1W8 View 3D Structure Click here
Q7SXX4 View 3D Structure Click here
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Q7ZUC2 View 3D Structure Click here
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Q800V7 View 3D Structure Click here
Q80X60 View 3D Structure Click here
Q84SL0 View 3D Structure Click here
Q86JE1 View 3D Structure Click here
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Q8BG18 View 3D Structure Click here
Q8BH97 View 3D Structure Click here
Q8IQ15 View 3D Structure Click here
Q8IR92 View 3D Structure Click here
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Q9CQ19 View 3D Structure Click here
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Q9FI43 View 3D Structure Click here
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Q9JJ69 View 3D Structure Click here
Q9JM47 View 3D Structure Click here
Q9JM59 View 3D Structure Click here
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