Summary: HrpE/YscL/FliH and V-type ATPase subunit E
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HrpE/YscL/FliH and V-type ATPase subunit E Provide feedback
This is a prokaryotic family that contains proteins of the FliH and HrpE/YscL family. These proteins are involved in type III secretion, which is the process that drives flagellar biosynthesis and mediates bacterial-eukaryotic interactions [1-2]. This family also V-type ATPase subunit E. This subunit appears to form a tight interaction with subunit G in the F0 complex [3]. Subunits E and G may act together as stators to prevent certain subunits from rotating with the central rotary element [3]. PF01991 also contains V-type ATPase subunit E proteins.
Literature references
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Huang HC, Lin RH, Chang CJ, Collmer A, Deng WL; , Mol Plant Microbe Interact 1995;8:733-746.: The complete hrp gene cluster of Pseudomonas syringae pv. syringae 61 includes two blocks of genes required for harpinPss secretion that are arranged colinearly with Yersinia ysc homologs. PUBMED:7579617 EPMC:7579617
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Pallen MJ, Bailey CM, Beatson SA; , Protein Sci. 2006;15:935-941.: Evolutionary links between FliH/YscL-like proteins from bacterial type III secretion systems and second-stalk components of the FoF1 and vacuolar ATPases. PUBMED:16522800 EPMC:16522800
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Fethiere J, Venzke D, Diepholz M, Seybert A, Geerlof A, Gentzel M, Wilm M, Bottcher B;, J Biol Chem. 2004;279:40670-40676.: Building the stator of the yeast vacuolar-ATPase: specific interaction between subunits E and G. PUBMED:15292229 EPMC:15292229
Internal database links
SCOOP: | ATP-synt_B DivIVA FliH NolV V-ATPase_G_2 vATP-synt_E |
This tab holds annotation information from the InterPro database.
InterPro entry IPR009335
This family consists of several bacterial HrpE proteins, which are believed to function on the type III secretion system, specifically the secretion of HrpZ (harpinPss) [PUBMED:9045830]. This family also includes V-type proton ATPase subunit E proteins. This subunit appears to form a tight interaction with subunit G in the F0 complex. Subunits E and G may act together as stators to prevent certain subunits from rotating with the central rotary element [PUBMED:15292229]. also contains V-type ATPase subunit E proteins.
There is an evolutionary link between type III secretion systems and membrane-associated proton translocating ATPases [PUBMED:16522800].
Domain organisation
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Pfam Clan
This family is a member of clan ATP_synthase (CL0255), which has the following description:
This clan contains subunits of the F0 complex of ATP-synthase. The F0 complex is the non-catalytic unit of ATPase and is involved in proton translocation across membranes.
The clan contains the following 13 members:
ATP-synt_8 ATP-synt_B FliH Fun_ATP-synt_8 HrpE Mt_ATP-synt_B NolV OSCP V-ATPase_G V-ATPase_G_2 vATP-synt_E Yae1_N YMF19Alignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (4) |
Full (52) |
Representative proteomes | UniProt (378) |
NCBI (1052) |
Meta (18) |
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RP15 (12) |
RP35 (24) |
RP55 (53) |
RP75 (147) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (4) |
Full (52) |
Representative proteomes | UniProt (378) |
NCBI (1052) |
Meta (18) |
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RP15 (12) |
RP35 (24) |
RP55 (53) |
RP75 (147) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_11055 (release 9.0) |
Previous IDs: | none |
Type: | Family |
Author: | Moxon SJ |
Number in seed: | 4 |
Number in full: | 52 |
Average length of the domain: | 168.00 aa |
Average identity of full alignment: | 18 % |
Average coverage of the sequence by the domain: | 83.40 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 191 | ||||||||||||
Family (HMM) version: | 11 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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