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1  structure 4162  species 0  interactions 4308  sequences 7  architectures

Family: IF3_N (PF05198)

Summary: Translation initiation factor IF-3, N-terminal domain

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This is the Wikipedia entry entitled "Translation initiation factor IF-3". More...

Translation initiation factor IF-3 Edit Wikipedia article

Translation initiation factor IF-3, N-terminal domain
PDB 1tif EBI.jpg
translation initiation factor 3 n-terminal domain
Identifiers
Symbol IF3_N
Pfam PF05198
InterPro IPR019814
PROSITE PDOC00723
SCOP 1tif
SUPERFAMILY 1tif
Translation initiation factor IF-3, C-terminal domain
PDB 1tig EBI.jpg
translation initiation factor 3 c-terminal domain
Identifiers
Symbol IF3_C
Pfam PF00707
InterPro IPR019815
PROSITE PDOC00723
SCOP 1tif
SUPERFAMILY 1tif

In molecular biology, translation initiation factor IF-3 (gene infC) is one of the three factors required for the initiation of protein biosynthesis in bacteria. IF-3 is thought to function as a fidelity factor during the assembly of the ternary initiation complex which consists of the 30S ribosomal subunit, the initiator tRNA and the messenger RNA. IF-3 is a basic protein that binds to the 30S ribosomal subunit.[1] The chloroplast initiation factor IF-3(chl) is a protein that enhances the poly(A,U,G)-dependent binding of the initiator tRNA to chloroplast ribosomal 30s subunits in which the central section is evolutionary related to the sequence of bacterial IF-3.[2]


References[edit]

  1. ^ Liveris D, Schwartz JJ, Geertman R, Schwartz I (September 1993). "Molecular cloning and sequencing of infC, the gene encoding translation initiation factor IF3, from four enterobacterial species". FEMS Microbiol. Lett. 112 (2): 211–6. PMID 8405963. 
  2. ^ Lin Q, Ma L, Burkhart W, Spremulli LL (April 1994). "Isolation and characterization of cDNA clones for chloroplast translational initiation factor-3 from Euglena gracilis". J. Biol. Chem. 269 (13): 9436–44. PMID 8144528. 

This article incorporates text from the public domain Pfam and InterPro IPR019815

This article incorporates text from the public domain Pfam and InterPro IPR019814

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

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External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR019814

Initiation factor 3 (IF-3) (gene infC) is one of the three factors required for the initiation of protein biosynthesis in bacteria. IF-3 is thought to function as a fidelity factor during the assembly of the ternary initiation complex which consist of the 30S ribosomal subunit, the initiator tRNA and the messenger RNA. IF-3 is a basic protein that binds to the 30S ribosomal subunit [PUBMED:8405963]. The chloroplast initiation factor IF-3(chl) is a protein that enhances the poly(A,U,G)-dependent binding of the initiator tRNA to chloroplast ribosomal 30s subunits in which the central section is evolutionary related to the sequence of bacterial IF-3 [PUBMED:8144528].

Gene Ontology

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Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

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(181)
Full
(4308)
Representative proteomes NCBI
(2633)
Meta
(2165)
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(369)
RP35
(701)
RP55
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RP75
(1055)
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Format an alignment

  Seed
(181)
Full
(4308)
Representative proteomes NCBI
(2633)
Meta
(2165)
RP15
(369)
RP35
(701)
RP55
(898)
RP75
(1055)
Alignment:
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  Seed
(181)
Full
(4308)
Representative proteomes NCBI
(2633)
Meta
(2165)
RP15
(369)
RP35
(701)
RP55
(898)
RP75
(1055)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

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Curation and family details

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Seed source: Pfam-B_629 (release 2.1)
Previous IDs: none
Type: Domain
Author: Bateman A, Finn RD
Number in seed: 181
Number in full: 4308
Average length of the domain: 67.60 aa
Average identity of full alignment: 52 %
Average coverage of the sequence by the domain: 37.87 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.3 21.3
Trusted cut-off 21.4 21.3
Noise cut-off 21.2 20.9
Model length: 76
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the IF3_N domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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