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0  structures 49  species 0  interactions 335  sequences 5  architectures

Family: Ins_allergen_rp (PF06757)

Summary: Insect allergen related repeat, nitrile-specifier detoxification

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Insect allergen related repeat, nitrile-specifier detoxification Provide feedback

This family exemplifies a case of novel gene evolution. The case in point is the arms-race between plants and their infective insective herbivores in the area of the glucosinolate-myrosinase system. Brassicas have developed the glucosinolate-myrosinase system as chemical defence mechanism against the insects, and consequently the insects have adapted to produce a detoxifying molecule, nitrile-specifier protein (NSP). NSP is present in the small white butterfly Pieris rapae. NSP is structurally different from and has no amino acid homology to any known detoxifying enzymes, and it appears to have arisen by a process of domain and gene duplication of a sequence of unknown function that is widespread in insect species and referred to as insect-allergen-repeat protein. Thus this family is found either as a single domain or as a multiple repeat-domain [3].

Literature references

  1. Wang NM, Lee MF, Wu CH; , Allergy 1999;54:119-127.: Immunologic characterization of a recombinant American cockroach (Periplaneta americana) Per a 1 (Cr-PII) allergen. PUBMED:10221434 EPMC:10221434

  2. Wu CH, Wang NM, Lee MF, Kao CY, Luo SF; , J Allergy Clin Immunol 1998;101:832-840.: Cloning of the American cockroach Cr-PII allergens: evidence for the existence of cross-reactive allergens between species. PUBMED:9648712 EPMC:9648712

  3. Fischer HM, Wheat CW, Heckel DG, Vogel H; , Mol Biol Evol. 2008;25:809-820.: Evolutionary origins of a novel host plant detoxification gene in butterflies. PUBMED:18296701 EPMC:18296701


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR010629

This entry represents several insect specific allergen repeats. These repeats are commonly found in various proteins from cockroaches, fruit flies and mosquitos. It has been suggested that the repeat sequences have evolved by duplication of an ancestral amino acid domain, which may have arisen from the mitochondrial energy transfer proteins [PUBMED:9804858].

This family exemplifies a case of novel gene evolution. The case in point is the arms-race between plants and their infective insective herbivores in the area of the glucosinolate-myrosinase system. Brassicas have developed the glucosinolate-myrosinase system as chemical defence mechanism against the insects, and consequently the insects have adapted to produce a detoxifying molecule, nitrile-specifier protein (NSP). NSP is present in the Pieris rapae (Cabbage white butterfly). NSP is structurally different from and has no amino acid homology to any known detoxifying enzymes, and it appears to have arisen by a process of domain and gene duplication of a sequence of unknown function that is widespread in insect species and referred to as insect-allergen-repeat protein. Thus this family is found either as a single domain or as a multiple repeat-domain [PUBMED:18296701].

Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(38)
Full
(335)
Representative proteomes NCBI
(369)
Meta
(0)
RP15
(31)
RP35
(38)
RP55
(108)
RP75
(131)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(38)
Full
(335)
Representative proteomes NCBI
(369)
Meta
(0)
RP15
(31)
RP35
(38)
RP55
(108)
RP75
(131)
Alignment:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(38)
Full
(335)
Representative proteomes NCBI
(369)
Meta
(0)
RP15
(31)
RP35
(38)
RP55
(108)
RP75
(131)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_5947 (release 10.0)
Previous IDs: none
Type: Family
Author: Moxon SJ
Number in seed: 38
Number in full: 335
Average length of the domain: 174.00 aa
Average identity of full alignment: 23 %
Average coverage of the sequence by the domain: 79.51 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.6 21.6
Trusted cut-off 21.6 22.7
Noise cut-off 21.0 21.2
Model length: 179
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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