Summary: Nuclear transport factor 2 (NTF2) domain
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Nuclear transport factor 2 (NTF2) domain Provide feedback
This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.
Stewart M, Kent HM, McCoy AJ; , J Mol Biol 1998;277:635-646.: Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran. PUBMED:9533885 EPMC:9533885
Internal database links
|Similarity to PfamA using HHSearch:||SnoaL_2 SnoaL_3 DUF4440|
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR002075
Nuclear transport factor 2 (NTF2) is a homodimer which stimulates efficient nuclear import of a cargo protein. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins. NTF2 folds into a cone with a deep hydrophobic cavity, the opening of which is surrounded by several negatively charged residues. RanGDP binds to NTF2 by inserting a conserved phenylalanine residue into the hydrophobic pocket of NTF2 and making electrostatic interactions with the conserved negatively charged residues that surround the cavity [PUBMED:9533885].
This entry represent the main structural domain of NTF2 and related domains which are found in other nuclear import proteins.
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Cellular component||intracellular (GO:0005622)|
|Biological process||transport (GO:0006810)|
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This superfamily contains a variety of enzymes such as Scytalone dehydratase, Delta-5-3-ketosteroid isomerase, Limonene-1,2-epoxide hydrolase among others. The family also includes presumed non-enzymatic homologues such as NTF2.
The clan contains the following 24 members:CaMKII_AD DUF1348 DUF2358 DUF3225 DUF3804 DUF4440 DUF4467 LEH Lumazine_bd Lumazine_bd_2 MBA1 MecA_N Mtr2 NTF2 PHZA_PHZB Ring_hydroxyl_B Scytalone_dh SnoaL SnoaL_2 SnoaL_3 SnoaL_4 Tim44 VirB8 WI12
We make a range of alignments for each Pfam-A family:
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Curation and family details
|Author:||Mian N, Bateman A, Griffiths-Jones SR|
|Number in seed:||43|
|Number in full:||1695|
|Average length of the domain:||119.60 aa|
|Average identity of full alignment:||22 %|
|Average coverage of the sequence by the domain:||36.72 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||15|
|Download:||download the raw HMM for this family|
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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the NTF2 domain has been found. There are 189 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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