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1219  structures 1756  species 0  interactions 2321  sequences 40  architectures

Family: Hexapep (PF00132)

Summary: Bacterial transferase hexapeptide (six repeats)

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Bacterial transferase hexapeptide (six repeats) Provide feedback

No Pfam abstract.

Literature references

  1. Raetz CR, Roderick SL; , Science 1995;270:997-1000.: A left-handed parallel beta helix in the structure of UDP-N-acetylglucosamine acyltransferase. PUBMED:7481807 EPMC:7481807

Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001451

A variety of bacterial transferases contain a repeat structure composed of tandem repeats of a [LIV]-G-X(4) hexapeptide, which, in the tertiary structure of LpxA (UDP N-acetylglucosamine acyltransferase) [ PUBMED:7481807 ], has been shown to form a left-handed parallel beta helix. A number of different transferase protein families contain this repeat, such as galactoside acetyltransferase-like proteins [ PUBMED:11937062 ], the gamma-class of carbonic anhydrases [ PUBMED:10924115 ], and tetrahydrodipicolinate-N-succinlytransferases (DapD), the latter containing an extra N-terminal 3-helical domain [ PUBMED:11910040 ]. It has been shown that most hexapeptide acyltransferases form catalytic trimers with three symmetrical active sites [ PUBMED:17519228 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan HEXAPEP (CL0536), which has the following description:

This superfamily is characterised by superhelical turns made up of three short strands duplicated many times; the sequence hexapeptide repeats correspond to individual strands. These hexapeptide repeat regions occur in a range of transferase enzymes.

The clan contains the following 5 members:

DUF4954 Fucokinase Hexapep Hexapep_2 Hexapep_loop


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

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Representative proteomes UniProt

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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: hexapep;
Type: Repeat
Sequence Ontology: SO:0001068
Author: Sonnhammer ELL
Number in seed: 216
Number in full: 2321
Average length of the domain: 36.00 aa
Average identity of full alignment: 43 %
Average coverage of the sequence by the domain: 15.47 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.5 17.1
Trusted cut-off 21.5 41.9
Noise cut-off 21.4 -1000000.0
Model length: 36
Family (HMM) version: 26
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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The tree shows the occurrence of this domain across different species. More...


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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Hexapep domain has been found. There are 1219 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A1D6MA66 View 3D Structure Click here
O74484 View 3D Structure Click here
Q0DGG8 View 3D Structure Click here
Q42538 View 3D Structure Click here
Q54ED9 View 3D Structure Click here
Q57752 View 3D Structure Click here