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268  structures 8306  species 0  interactions 27744  sequences 232  architectures

Family: GHMP_kinases_N (PF00288)

Summary: GHMP kinases N terminal domain

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This is the Wikipedia entry entitled "GHMP kinase family". More...

GHMP kinase family Edit Wikipedia article

GHMP kinases N terminal domain
PDB 1k47 EBI.jpg
crystal structure of the streptococcus pneumoniae phosphomevalonate kinase (pmk)
Symbol GHMP_kinases_N
Pfam PF00288
Pfam clan CL0329
InterPro IPR006204
SCOP 1fwl
GHMP kinases C terminal
PDB 1s4e EBI.jpg
pyrococcus furiosus galactokinase in complex with galactose, adp and magnesium
Symbol GHMP_kinases_C
Pfam PF08544
InterPro IPR013750
SCOP 1fwl

In molecular biology, the GHMP kinase family is a family of kinase enzymes. Members of this family include homoserine kinases EC, galactokinases EC, and mevalonate kinasesEC These kinases make up the GHMP kinase superfamily of ATP-dependent enzymes.[1] These enzymes are involved in the biosynthesis of isoprenes and amino acids as well as in carbohydrate metabolism. These enzymes contain, in their N-terminal section, a conserved Gly/Ser-rich region which is probably involved in the binding of ATP.[2][3] The C-terminal domain of homoserine kinase has a central alpha-beta plait fold and an insertion of four helices, which, together with the N-terminal fold, creates a novel nucleotide binding fold.[4]


  1. ^ Bork P, Sander C, Valencia A (January 1993). "Convergent evolution of similar enzymatic function on different protein folds: the hexokinase, ribokinase, and galactokinase families of sugar kinases". Protein Sci. 2 (1): 31–40. doi:10.1002/pro.5560020104. PMC 2142297Freely accessible. PMID 8382990. 
  2. ^ Tsay YH, Robinson GW (February 1991). "Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase". Mol. Cell. Biol. 11 (2): 620–31. PMC 359713Freely accessible. PMID 1846667. 
  3. ^ Lee M, Leustek T (December 1999). "Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene". Arch. Biochem. Biophys. 372 (1): 135–42. doi:10.1006/abbi.1999.1481. PMID 10562426. 
  4. ^ Zhou T, Daugherty M, Grishin NV, Osterman AL, Zhang H (December 2000). "Structure and mechanism of homoserine kinase: prototype for the GHMP kinase superfamily". Structure. 8 (12): 1247–57. doi:10.1016/s0969-2126(00)00533-5. PMID 11188689. 

This article incorporates text from the public domain Pfam and InterPro IPR013750

This article incorporates text from the public domain Pfam and InterPro IPR006204

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

GHMP kinases N terminal domain Provide feedback

This family includes homoserine kinases, galactokinases and mevalonate kinases.

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR006204

The galacto- ( EC ), homoserine ( EC ), mevalonate ( EC ) and phosphomevalonate ( EC ) kinases contain, in their N-terminal section, a conserved domain with a Gly/Ser-rich region which is probably involved in the binding of ATP [ PUBMED:1846667 , PUBMED:10562426 ]. This group of kinases has been called 'GHMP' (from the first letter of their substrates).

This domain is also found in diphosphomevalonate decarboxylases, which are structurally related members of the GHMP superfamily [ PUBMED:17583736 ], but do not possess kinase activity.

Gene Ontology

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Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan S5 (CL0329), which has the following description:

This superfamily contains a wide range of families that possess a structure similar to the second domain of ribosomal S5 protein.

The clan contains the following 18 members:

ChlI DNA_gyraseB DNA_mis_repair EFG_IV Fae GalKase_gal_bdg GHMP_kinases_N IGPD Lon_C LpxC Morc6_S5 Ribonuclease_P Ribosomal_S5_C Ribosomal_S9 RNase_PH Topo-VIb_trans UPF0029 Xol-1_N


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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: GHMP_kinases;
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD , Mistry J
Number in seed: 447
Number in full: 27744
Average length of the domain: 65.80 aa
Average identity of full alignment: 23 %
Average coverage of the sequence by the domain: 17.07 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.3 22.3
Trusted cut-off 22.3 22.3
Noise cut-off 22.2 22.2
Model length: 66
Family (HMM) version: 28
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the GHMP_kinases_N domain has been found. There are 268 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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