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171  structures 5356  species 0  interactions 42669  sequences 1047  architectures

Family: FHA (PF00498)

Summary: FHA domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Forkhead-associated domain". More...

Forkhead-associated domain Edit Wikipedia article

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

FHA domain Provide feedback

The FHA (Forkhead-associated) domain is a phosphopeptide binding motif [2].

Literature references

  1. Hofmann K, Bucher, P; , Trends Biochem Sci 1995;20:347-349.: The FHA domain: a putative nuclear signalling domain found in protein kinases and transcription factors. PUBMED:7482699 EPMC:7482699

  2. Durocher D, Henckel J, Fersht AR, Jackson SP; , Mol Cell. 1999;4:387-394.: The FHA domain is a modular phosphopeptide recognition motif. PUBMED:10518219 EPMC:10518219

  3. Pallen M, Chaudhuri R, Khan A; , Trends Microbiol 2002;10:556-563.: Bacterial FHA domains: neglected players in the phospho-threonine signalling game? PUBMED:12564991 EPMC:12564991


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000253

The forkhead-associated (FHA) domain [ PUBMED:7482699 ] is a phosphopeptide recognition domain found in many regulatory proteins. It displays specificity for phosphothreonine-containing epitopes but will also recognise phosphotyrosine with relatively high affinity. It spans approximately 80-100 amino acid residues folded into an 11-stranded beta sandwich, which sometimes contain small helical insertions between the loops connecting the strands [ PUBMED:11911881 ].

To date, genes encoding FHA-containing proteins have been identified in eubacterial and eukaryotic but not archaeal genomes. The domain is present in a diverse range of proteins, such as kinases, phosphatases, kinesins, transcription factors, RNA-binding proteins and metabolic enzymes which partake in many different cellular processes - DNA repair, signal transduction, vesicular transport and protein degradation are just a few examples.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan SMAD-FHA (CL0357), which has the following description:

Superfamily members carry a few short helices inserted in loops within the 11 strands in 2 sheets (greek-key) of the parent fold.

The clan contains the following 9 members:

FHA FHA_2 FtsK_SpoIIIE_N IRF-3 MH2 MU2_FHA Pellino PrgH Yop-YscD_cpl

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(193)
Full
(42669)
Representative proteomes UniProt
(119464)
RP15
(7122)
RP35
(19858)
RP55
(38556)
RP75
(57763)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(193)
Full
(42669)
Representative proteomes UniProt
(119464)
RP15
(7122)
RP35
(19858)
RP55
(38556)
RP75
(57763)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(193)
Full
(42669)
Representative proteomes UniProt
(119464)
RP15
(7122)
RP35
(19858)
RP55
(38556)
RP75
(57763)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD , Durocher D
Number in seed: 193
Number in full: 42669
Average length of the domain: 71.50 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 10.81 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.7 20.7
Trusted cut-off 20.7 20.7
Noise cut-off 20.6 20.6
Model length: 69
Family (HMM) version: 28
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the FHA domain has been found. There are 171 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A060D6M9 View 3D Structure Click here
A0A0G2K8Z9 View 3D Structure Click here
A0A0P0WQD6 View 3D Structure Click here
A0A0P0XNS7 View 3D Structure Click here
A0A0P0XZB0 View 3D Structure Click here
A0A0R0ECK4 View 3D Structure Click here
A0A0R0F2U5 View 3D Structure Click here
A0A0R0IF24 View 3D Structure Click here
A0A0R0IMP6 View 3D Structure Click here
A0A0R0JPK0 View 3D Structure Click here
A0A0R0L0E9 View 3D Structure Click here
A0A0R0LCM6 View 3D Structure Click here
A0A0R4IM96 View 3D Structure Click here
A0A140UHW9 View 3D Structure Click here
A0A1D5NSL5 View 3D Structure Click here
A0A1D6E9L1 View 3D Structure Click here
A0A1D6EHK9 View 3D Structure Click here
A0A1D6EL89 View 3D Structure Click here
A0A1D6F7T3 View 3D Structure Click here
A0A1D6F810 View 3D Structure Click here
A0A1D6GFF6 View 3D Structure Click here
A0A1D6GI88 View 3D Structure Click here
A0A1D6GLZ3 View 3D Structure Click here
A0A1D6GP16 View 3D Structure Click here
A0A1D6HUR4 View 3D Structure Click here
A0A1D6HW02 View 3D Structure Click here
A0A1D6J7P5 View 3D Structure Click here
A0A1D6JMB1 View 3D Structure Click here
A0A1D6KJS7 View 3D Structure Click here
A0A1D6M2D7 View 3D Structure Click here
A0A1D6NQK6 View 3D Structure Click here
A0A1D6PNJ3 View 3D Structure Click here
A0A1D6Q178 View 3D Structure Click here
A0A1D8PD53 View 3D Structure Click here
A0A1D8PDJ0 View 3D Structure Click here
A0A1D8PF67 View 3D Structure Click here
A0A1D8PI13 View 3D Structure Click here
A0A1D8PJZ1 View 3D Structure Click here
A0A1D8PJZ1 View 3D Structure Click here
A0A1D8PP50 View 3D Structure Click here
A0A1D8PPB7 View 3D Structure Click here
A0A286Y8S4 View 3D Structure Click here
A0A286YCV9 View 3D Structure Click here
A0A2R8QEM9 View 3D Structure Click here
A0A2R8QF74 View 3D Structure Click here
A0A368UI45 View 3D Structure Click here
A1ZAJ2 View 3D Structure Click here
A2VEV2 View 3D Structure Click here
A4F324 View 3D Structure Click here
A4HS61 View 3D Structure Click here
A4HSR2 View 3D Structure Click here
A4HTT2 View 3D Structure Click here
A4HWT1 View 3D Structure Click here
A4I483 View 3D Structure Click here
A4IAJ5 View 3D Structure Click here
A5PF58 View 3D Structure Click here
A5WW08 View 3D Structure Click here
A6PWD2 View 3D Structure Click here
B0S7L9 View 3D Structure Click here
B0V1J5 View 3D Structure Click here
B1AJZ9 View 3D Structure Click here
B1AVY7 View 3D Structure Click here
B1WC70 View 3D Structure Click here
B4Y5J6 View 3D Structure Click here
B4Y5J7 View 3D Structure Click here
B7F9R3 View 3D Structure Click here
B7SY83 View 3D Structure Click here
B7Z0D7 View 3D Structure Click here
B7ZXC0 View 3D Structure Click here
C0H5H1 View 3D Structure Click here
D3YXS5 View 3D Structure Click here
D3ZE01 View 3D Structure Click here
D3ZFN9 View 3D Structure Click here
D3ZTF1 View 3D Structure Click here
D3ZU55 View 3D Structure Click here
D4A1G8 View 3D Structure Click here
E2QCZ5 View 3D Structure Click here
E7F084 View 3D Structure Click here
E7F3N4 View 3D Structure Click here
E7F3T1 View 3D Structure Click here
E7F4R0 View 3D Structure Click here
E7FA74 View 3D Structure Click here
E7FD52 View 3D Structure Click here
E7FFM9 View 3D Structure Click here
E9AH34 View 3D Structure Click here
E9PX68 View 3D Structure Click here
F1M4A4 View 3D Structure Click here
F1MAK0 View 3D Structure Click here
F1Q554 View 3D Structure Click here
F1Q7J5 View 3D Structure Click here
F1QBV5 View 3D Structure Click here
F1QME9 View 3D Structure Click here
F1QSV6 View 3D Structure Click here
F1RA07 View 3D Structure Click here
F1RD88 View 3D Structure Click here
F1RDJ3 View 3D Structure Click here
F4HPY1 View 3D Structure Click here
F4HZ48 View 3D Structure Click here
F4IG63 View 3D Structure Click here
F4JCV6 View 3D Structure Click here
F6NHV5 View 3D Structure Click here
F8W2L7 View 3D Structure Click here
F8WLE0 View 3D Structure Click here
G3V9E0 View 3D Structure Click here
G5ED60 View 3D Structure Click here
I1JCK3 View 3D Structure Click here
I1JD80 View 3D Structure Click here
I1JUA1 View 3D Structure Click here
I1KZ40 View 3D Structure Click here
I1L057 View 3D Structure Click here
I1L2F4 View 3D Structure Click here
I1LHE5 View 3D Structure Click here
I1MBP3 View 3D Structure Click here
I1MFU3 View 3D Structure Click here
I1MN89 View 3D Structure Click here
I1MNY3 View 3D Structure Click here
I1MVX7 View 3D Structure Click here
I1N4T2 View 3D Structure Click here
I1NDS2 View 3D Structure Click here
K7KHS6 View 3D Structure Click here
K7KL57 View 3D Structure Click here
K7KSQ3 View 3D Structure Click here
K7L2T3 View 3D Structure Click here
K7L8U3 View 3D Structure Click here
K7LKQ9 View 3D Structure Click here
K7M0B0 View 3D Structure Click here
K7MLF2 View 3D Structure Click here
K7MR26 View 3D Structure Click here
K7MSL1 View 3D Structure Click here
K7TW25 View 3D Structure Click here
K7W397 View 3D Structure Click here
L0N7N1 View 3D Structure Click here
O14270 View 3D Structure Click here
O23305 View 3D Structure Click here
O35071 View 3D Structure Click here
O35787 View 3D Structure Click here
O35889 View 3D Structure Click here
O43070 View 3D Structure Click here
O43896 View 3D Structure Click here
O50389 View 3D Structure Click here
O60129 View 3D Structure Click here
O60333 View 3D Structure Click here
O60934 View 3D Structure Click here
O61267 View 3D Structure Click here
O61817 View 3D Structure Click here
O65934 View 3D Structure Click here
O65934 View 3D Structure Click here
O74388 View 3D Structure Click here
O76064 View 3D Structure Click here
O88658 View 3D Structure Click here
O96017 View 3D Structure Click here
P0C219 View 3D Structure Click here
P22216 View 3D Structure Click here
P22216 View 3D Structure Click here
P24719 View 3D Structure Click here
P33173 View 3D Structure Click here
P33301 View 3D Structure Click here
P34101 View 3D Structure Click here
P34648 View 3D Structure Click here
P38823 View 3D Structure Click here
P39009 View 3D Structure Click here
P39521 View 3D Structure Click here
P40466 View 3D Structure Click here
P41813 View 3D Structure Click here
P42128 View 3D Structure Click here
P46012 View 3D Structure Click here
P46014 View 3D Structure Click here
P53156 View 3D Structure Click here
P53924 View 3D Structure Click here
P55196 View 3D Structure Click here
P71590 View 3D Structure Click here
P85037 View 3D Structure Click here
P91125 View 3D Structure Click here
P9WGJ9 View 3D Structure Click here
P9WJA9 View 3D Structure Click here
Q01167 View 3D Structure Click here
Q03944 View 3D Structure Click here
Q04383 View 3D Structure Click here
Q06001 View 3D Structure Click here
Q06266 View 3D Structure Click here
Q07930 View 3D Structure Click here
Q09170 View 3D Structure Click here
Q0H8D7 View 3D Structure Click here
Q0J485 View 3D Structure Click here
Q0JCU7 View 3D Structure Click here
Q0WW94 View 3D Structure Click here
Q10292 View 3D Structure Click here
Q10322 View 3D Structure Click here
Q10SC2 View 3D Structure Click here
Q12756 View 3D Structure Click here
Q12972 View 3D Structure Click here
Q14676 View 3D Structure Click here
Q14BN4 View 3D Structure Click here
Q15058 View 3D Structure Click here
Q1ZXH2 View 3D Structure Click here
Q2QYD1 View 3D Structure Click here
Q3UCQ1 View 3D Structure Click here
Q3URD3 View 3D Structure Click here
Q4CPR4 View 3D Structure Click here
Q4CX35 View 3D Structure Click here
Q4CZH5 View 3D Structure Click here
Q4D0J8 View 3D Structure Click here
Q4D9J4 View 3D Structure Click here
Q4D9Y0 View 3D Structure Click here
Q4DAT8 View 3D Structure Click here
Q4DC95 View 3D Structure Click here
Q4DFU3 View 3D Structure Click here
Q4DH56 View 3D Structure Click here
Q4DPN7 View 3D Structure Click here
Q4DRP0 View 3D Structure Click here
Q4DV65 View 3D Structure Click here
Q4DWS7 View 3D Structure Click here
Q4DZ20 View 3D Structure Click here
Q4DZE5 View 3D Structure Click here
Q4E2Y6 View 3D Structure Click here
Q4E3M2 View 3D Structure Click here
Q4E413 View 3D Structure Click here
Q4KLN8 View 3D Structure Click here
Q4V8W8 View 3D Structure Click here
Q53P84 View 3D Structure Click here
Q54BF0 View 3D Structure Click here
Q54C63 View 3D Structure Click here
Q54DK7 View 3D Structure Click here
Q54ER5 View 3D Structure Click here
Q54FK0 View 3D Structure Click here
Q54MH0 View 3D Structure Click here
Q54PF7 View 3D Structure Click here
Q54ST8 View 3D Structure Click here
Q54VI1 View 3D Structure Click here
Q54VU4 View 3D Structure Click here
Q54WT0 View 3D Structure Click here
Q54X17 View 3D Structure Click here
Q54ZS7 View 3D Structure Click here
Q55C08 View 3D Structure Click here
Q55EZ0 View 3D Structure Click here
Q59ZC8 View 3D Structure Click here
Q5A7S7 View 3D Structure Click here
Q5BK67 View 3D Structure Click here
Q5I2W8 View 3D Structure Click here
Q5M9G6 View 3D Structure Click here
Q5PQJ8 View 3D Structure Click here
Q5PSV9 View 3D Structure Click here
Q5SW79 View 3D Structure Click here
Q5U2M8 View 3D Structure Click here
Q5VR92 View 3D Structure Click here
Q5XIB9 View 3D Structure Click here
Q60575 View 3D Structure Click here
Q6A065 View 3D Structure Click here
Q6ATJ5 View 3D Structure Click here
Q6ATQ8 View 3D Structure Click here
Q6P0E3 View 3D Structure Click here
Q6Z4I2 View 3D Structure Click here
Q6ZNK6 View 3D Structure Click here
Q793I8 View 3D Structure Click here
Q7K0W0 View 3D Structure Click here
Q7SYM1 View 3D Structure Click here
Q7TN31 View 3D Structure Click here
Q7XD82 View 3D Structure Click here
Q803C1 View 3D Structure Click here
Q80U49 View 3D Structure Click here
Q810L3 View 3D Structure Click here
Q84MA4 View 3D Structure Click here
Q851R6 View 3D Structure Click here
Q86IG6 View 3D Structure Click here
Q86NJ4 View 3D Structure Click here
Q8BIZ6 View 3D Structure Click here
Q8GWP4 View 3D Structure Click here
Q8H4Y5 View 3D Structure Click here
Q8I355 View 3D Structure Click here
Q8IEN7 View 3D Structure Click here
Q8II28 View 3D Structure Click here
Q8IR47 View 3D Structure Click here
Q8JZM6 View 3D Structure Click here
Q8N302 View 3D Structure Click here
Q8R3G1 View 3D Structure Click here
Q8TAD8 View 3D Structure Click here
Q8VC56 View 3D Structure Click here
Q8W4D8 View 3D Structure Click here
Q96CG3 View 3D Structure Click here
Q96EP1 View 3D Structure Click here
Q96EZ8 View 3D Structure Click here
Q96L93 View 3D Structure Click here
Q99KJ5 View 3D Structure Click here
Q99L90 View 3D Structure Click here
Q9BWU0 View 3D Structure Click here
Q9FGC7 View 3D Structure Click here
Q9FIK2 View 3D Structure Click here
Q9H1H9 View 3D Structure Click here
Q9JIL9 View 3D Structure Click here
Q9M8A0 View 3D Structure Click here
Q9NQT8 View 3D Structure Click here
Q9P7G6 View 3D Structure Click here
Q9QZQ1 View 3D Structure Click here
Q9R019 View 3D Structure Click here
Q9R207 View 3D Structure Click here
Q9SFV2 View 3D Structure Click here
Q9SFV6 View 3D Structure Click here
Q9U1Y5 View 3D Structure Click here
Q9U3Q2 View 3D Structure Click here
Q9V7W9 View 3D Structure Click here
Q9VIP4 View 3D Structure Click here
Q9VN82 View 3D Structure Click here
Q9VT40 View 3D Structure Click here
Q9VZJ3 View 3D Structure Click here
Q9W5Q1 View 3D Structure Click here
Q9W5R5 View 3D Structure Click here
Q9Y242 View 3D Structure Click here
Q9Y4F5 View 3D Structure Click here
Q9Z265 View 3D Structure Click here
U4PEB6 View 3D Structure Click here
X1WFZ0 View 3D Structure Click here
X1WHC0 View 3D Structure Click here