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178  structures 5508  species 0  interactions 48504  sequences 1075  architectures

Family: FHA (PF00498)

Summary: FHA domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Forkhead-associated domain". More...

Forkhead-associated domain Edit Wikipedia article

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

FHA domain Provide feedback

The FHA (Forkhead-associated) domain is a phosphopeptide binding motif [2].

Literature references

  1. Hofmann K, Bucher, P; , Trends Biochem Sci 1995;20:347-349.: The FHA domain: a putative nuclear signalling domain found in protein kinases and transcription factors. PUBMED:7482699 EPMC:7482699

  2. Durocher D, Henckel J, Fersht AR, Jackson SP; , Mol Cell. 1999;4:387-394.: The FHA domain is a modular phosphopeptide recognition motif. PUBMED:10518219 EPMC:10518219

  3. Pallen M, Chaudhuri R, Khan A; , Trends Microbiol 2002;10:556-563.: Bacterial FHA domains: neglected players in the phospho-threonine signalling game? PUBMED:12564991 EPMC:12564991


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000253

The forkhead-associated (FHA) domain [ PUBMED:7482699 ] is a phosphopeptide recognition domain found in many regulatory proteins. It displays specificity for phosphothreonine-containing epitopes but will also recognise phosphotyrosine with relatively high affinity. It spans approximately 80-100 amino acid residues folded into an 11-stranded beta sandwich, which sometimes contain small helical insertions between the loops connecting the strands [ PUBMED:11911881 ].

To date, genes encoding FHA-containing proteins have been identified in eubacterial and eukaryotic but not archaeal genomes. The domain is present in a diverse range of proteins, such as kinases, phosphatases, kinesins, transcription factors, RNA-binding proteins and metabolic enzymes which partake in many different cellular processes - DNA repair, signal transduction, vesicular transport and protein degradation are just a few examples.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan SMAD-FHA (CL0357), which has the following description:

Superfamily members carry a few short helices inserted in loops within the 11 strands in 2 sheets (greek-key) of the parent fold.

The clan contains the following 9 members:

FHA FHA_2 FtsK_SpoIIIE_N IRF-3 MH2 MU2_FHA Pellino PrgH Yop-YscD_cpl

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(193)
Full
(48504)
Representative proteomes UniProt
(127795)
RP15
(8101)
RP35
(21552)
RP55
(42194)
RP75
(63023)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(193)
Full
(48504)
Representative proteomes UniProt
(127795)
RP15
(8101)
RP35
(21552)
RP55
(42194)
RP75
(63023)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(193)
Full
(48504)
Representative proteomes UniProt
(127795)
RP15
(8101)
RP35
(21552)
RP55
(42194)
RP75
(63023)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prosite
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD , Durocher D
Number in seed: 193
Number in full: 48504
Average length of the domain: 71.50 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 9.88 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.7 20.7
Trusted cut-off 20.7 20.7
Noise cut-off 20.6 20.6
Model length: 69
Family (HMM) version: 29
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the FHA domain has been found. There are 178 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A060D6M9 View 3D Structure Click here
A0A0G2K8Z9 View 3D Structure Click here
A0A0P0WQD6 View 3D Structure Click here
A0A0P0XNS7 View 3D Structure Click here
A0A0P0XZB0 View 3D Structure Click here
A0A0R0ECK4 View 3D Structure Click here
A0A0R0F2U5 View 3D Structure Click here
A0A0R0IF24 View 3D Structure Click here
A0A0R0IMP6 View 3D Structure Click here
A0A0R0JPK0 View 3D Structure Click here
A0A0R0L0E9 View 3D Structure Click here
A0A0R0LCM6 View 3D Structure Click here
A0A0R4IM96 View 3D Structure Click here
A0A140UHW9 View 3D Structure Click here
A0A1D5NSL5 View 3D Structure Click here
A0A1D6E9L1 View 3D Structure Click here
A0A1D6EHK9 View 3D Structure Click here
A0A1D6EL89 View 3D Structure Click here
A0A1D6F7T3 View 3D Structure Click here
A0A1D6F810 View 3D Structure Click here
A0A1D6GFF6 View 3D Structure Click here
A0A1D6GI88 View 3D Structure Click here
A0A1D6GLZ3 View 3D Structure Click here
A0A1D6GP16 View 3D Structure Click here
A0A1D6HUR4 View 3D Structure Click here
A0A1D6HW02 View 3D Structure Click here
A0A1D6J7P5 View 3D Structure Click here
A0A1D6JMB1 View 3D Structure Click here
A0A1D6KJS7 View 3D Structure Click here
A0A1D6M2D7 View 3D Structure Click here
A0A1D6NQK6 View 3D Structure Click here
A0A1D6PNJ3 View 3D Structure Click here
A0A1D6Q178 View 3D Structure Click here
A0A1D8PD53 View 3D Structure Click here
A0A1D8PDJ0 View 3D Structure Click here
A0A1D8PF67 View 3D Structure Click here
A0A1D8PI13 View 3D Structure Click here
A0A1D8PJZ1 View 3D Structure Click here
A0A1D8PP50 View 3D Structure Click here
A0A1D8PPB7 View 3D Structure Click here