Summary: E1 Protein, N terminal domain
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E1 Protein, N terminal domain Provide feedback
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This tab holds annotation information from the InterPro database.
InterPro entry IPR014000
Papillomaviruses are a large family of DNA tumour viruses which give rise to warts in their host species. The helicase E1 protein is an ATP-dependent DNA helicase required for initiation of viral DNA replication [PUBMED:8389467]. It forms a complex with the viral E2 protein, which is a site-specific DNA-binding transcriptional activator. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins [PUBMED:2176744].
The E1 protein is a 70 kDa polypeptide with a centrally-located DNA-binding domain and a C-terminal ATPase/helicase domain. It binds specific 18 bp DNA sequences at the origin of replication, melts the DNA duplex and functions as a 3' to 5' helicase [PUBMED:9060646]. In addition to E2 it also interacts with DNA polymerase alpha and replication protein A to effect DNA replication. The DNA-binding domain forms a five-stranded antiparallel beta sheet bordered by four loosely packed alpha helices on one side and two tightly packed helices on the other [PUBMED:10949036]. Two structural modules within this domain, an extended loop and a helix, contain conserved residues and are critical for DNA binding. In solution E1 is a monomer, but binds DNA as a dimer. Recruitment of more E1 subunits to the complex leads to melting of the origin and ultimately to the formation of an E1 hexamer with helicase activity [PUBMED:9658141].
This entry represents the N-terminal region of E1, which contains the nuclear localisation signal.
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Molecular function||hydrolase activity, acting on acid anhydrides (GO:0016817)|
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Curation and family details
|Seed source:||Pfam-B_98 (release 1.0)|
|Number in seed:||76|
|Number in full:||514|
|Average length of the domain:||123.50 aa|
|Average identity of full alignment:||39 %|
|Average coverage of the sequence by the domain:||22.24 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||13|
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