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142  structures 4740  species 2  interactions 8489  sequences 297  architectures

Family: BRCT (PF00533)

Summary: BRCA1 C Terminus (BRCT) domain

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This is the Wikipedia entry entitled "BRCT domain". More...

BRCT domain Edit Wikipedia article

BRCA1 C Terminus (BRCT) domain
PDB 1cdz EBI.jpg
Structure of an XRCC1 BRCT domain.[1]
Identifiers
Symbol BRCT
Pfam PF00533
InterPro IPR001357
SCOP 1cdz
SUPERFAMILY 1cdz
CDD cd00027

BRCA1 C Terminus (BRCT) domain is a family of evolutionarily related proteins.

The BRCT domain (after the C_terminal domain of a breast cancer susceptibility protein) is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage,[2] for example as found in the breast cancer DNA-repair protein BRCA1. The domain is an approximately 100 amino acid tandem repeat, which appears to act as a phospho-protein binding domain.[3]

Examples[edit]

Human proteins containing this domain include:

References[edit]

  1. ^ Zhang X, Moréra S, Bates PA, et al. (November 1998). "Structure of an XRCC1 BRCT domain: a new protein-protein interaction module". EMBO J. 17 (21): 6404–11. doi:10.1093/emboj/17.21.6404. PMC 1170965. PMID 9799248. 
  2. ^ Bork P, Hofmann K, Koonin EV, Bucher P, Neuwald AF, Altschul SF (1997). "A superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins". FASEB J. 11 (1): 68–76. PMID 9034168. 
  3. ^ Yu X, Chini CC, He M, Mer G, Chen J (2003). "The BRCT domain is a phospho-protein binding domain". Science 302 (5645): 639–642. doi:10.1126/science.1088753. PMID 14576433. 

External links[edit]

This article incorporates text from the public domain Pfam and InterPro IPR001357


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

BRCA1 C Terminus (BRCT) domain Provide feedback

The BRCT domain is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage. The BRCT domain of XRCC1 forms a homodimer in the crystal structure. This suggests that pairs of BRCT domains associate as homo- or heterodimers. BRCT domains are often found as tandem-repeat pairs [2]. Structures of the BRCA1 BRCT domains revealed a basis for a widely utilised head-to-tail BRCT-BRCT oligomerisation mode [3]. This conserved tandem BRCT architecture facilitates formation of the canonical BRCT phospho-peptide interaction cleft at a groove between the BRCT domains. Disease associated missense and nonsense mutations in the BRCA1 BRCT domains disrupt peptide binding by directly occluding this peptide binding groove, or by disrupting key conserved BRCT core folding determinants [5].

Literature references

  1. Koonin EV, Altschul SF, Bork P; , Nature Genet 1996;13:266-268.: BRCA1 protein products ...Functional motifs... PUBMED:8673121 EPMC:8673121

  2. Glover JN, Williams RS, Lee MS; , Trends Biochem Sci. 2004;29:579-585.: Interactions between BRCT repeats and phosphoproteins: tangled up in two. PUBMED:15501676 EPMC:15501676

  3. Williams RS, Green R, Glover JN; , Nat Struct Biol. 2001;8:838-842.: Crystal structure of the BRCT repeat region from the breast cancer-associated protein BRCA1. PUBMED:11573086 EPMC:11573086

  4. Williams RS, Lee MS, Hau DD, Glover JN; , Nat Struct Mol Biol. 2004;11:519-525.: Structural basis of phosphopeptide recognition by the BRCT domain of BRCA1. PUBMED:15133503 EPMC:15133503

  5. Williams RS, Chasman DI, Hau DD, Hui B, Lau AY, Glover JN; , J Biol Chem. 2003;278:53007-53016.: Detection of protein folding defects caused by BRCA1-BRCT truncation and missense mutations. PUBMED:14534301 EPMC:14534301


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001357

The BRCT domain (after the C_terminal domain of a breast cancer susceptibility protein) is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage [PUBMED:9034168], for example as found in the breast cancer DNA-repair protein BRCA1. The domain is an approximately 100 amino acid tandem repeat, which appears to act as a phospho-protein binding domain [PUBMED:14576433].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan BRCT-like (CL0459), which has the following description:

The BRCT domain is found predominantly in proteins involved in cell cycle checkpoint functions responsive to DNA damage.

The clan contains the following 2 members:

BRCT PTCB-BRCT

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(114)
Full
(8489)
Representative proteomes NCBI
(8863)
Meta
(1950)
RP15
(1124)
RP35
(1906)
RP55
(2771)
RP75
(3410)
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Format an alignment

  Seed
(114)
Full
(8489)
Representative proteomes NCBI
(8863)
Meta
(1950)
RP15
(1124)
RP35
(1906)
RP55
(2771)
RP75
(3410)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(114)
Full
(8489)
Representative proteomes NCBI
(8863)
Meta
(1950)
RP15
(1124)
RP35
(1906)
RP55
(2771)
RP75
(3410)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: [3]
Previous IDs: none
Type: Family
Author: Bateman A
Number in seed: 114
Number in full: 8489
Average length of the domain: 78.30 aa
Average identity of full alignment: 25 %
Average coverage of the sequence by the domain: 11.50 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.2 21.2
Trusted cut-off 21.2 21.2
Noise cut-off 21.1 21.1
Model length: 78
Family (HMM) version: 21
Download: download the raw HMM for this family

Species distribution

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Interactions

There are 2 interactions for this family. More...

BRCT P53

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the BRCT domain has been found. There are 142 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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