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29  structures 380  species 1  interaction 1535  sequences 47  architectures

Family: DIX (PF00778)

Summary: DIX domain

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DIX domain Provide feedback

The DIX domain is present in Dishevelled and axin [2]. This domain is involved in homo- and hetero-oligomerisation. It is involved in the homo- oligomerisation of mouse axin O35625 [3]. The axin DIX domain also interacts with the dishevelled DIX domain [4]. The DIX domain has also been called the DAX domain.

Literature references

  1. Zeng L, Fagotto F, Zhang T, Hsu W, Vasicek TJ, Perry WL 3rd, Lee JJ, Tilghman SM, Gumbiner BM, Costantini F; , Cell 1997;90:181-192.: The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. PUBMED:9230313 EPMC:9230313

  2. Cadigan KM, Nusse R; , Genes Dev 1997;11:3286-3305.: Wnt signaling: a common theme in animal development. PUBMED:9407023 EPMC:9407023

  3. Sakanaka C, Williams LT; , J Biol Chem 1999;274:14090-14093.: Functional domains of axin. Importance of the C terminus as an oligomerization domain. PUBMED:10318824 EPMC:10318824

  4. Kishida S, Yamamoto H, Hino S, Ikeda S, Kishida M, Kikuchi A; , Mol Cell Biol 1999;19:4414-4422.: DIX domains of Dvl and axin are necessary for protein interactions and their ability to regulate beta-catenin stability. PUBMED:10330181 EPMC:10330181


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001158

Proteins of the dishevelled family (Dsh and Dvl) play a key role in the transduction of the Wg/Wnt signal from the cell surface to the nucleus: in response to Wnt signal, they block the degradation of beta- catenin by interacting with the scaffolding protein axin. The N terminus of proteins of the dishevelled family and the C terminus of proteins of the axin family share a region of homology of about 85 amino acids, which has been called DIX for DIshevelled and aXin [PUBMED:9407023]. The DIX domain is found associated with PDZ and DEP domains in proteins of the dishevelled family and with an RGS domain in proteins of the axin family. DIX has been shown to be a protein-protein interaction domain that is important for homo- and hetero-oligomerization of proteins of the dishevelled and axin families [PUBMED:10330181, PUBMED:10318824, PUBMED:11041490, PUBMED:11027605]. The DIX domain has also be shown to be a signalling module that can target proteins to actin stress fibres and cytoplasmic vesicles to control Wnt signalling [PUBMED:12384700].

The Dvl2 DIX domain has been shown to form a predominantly helical structure [PUBMED:12384700].

A third type of DIX domain-possessing protein, known as Coiled-coil-DIX1 (Ccd1) or Dixin, forms homomeric and heteromeric complexes with Dvl and Axin and is positive regulator of Wnt signaling [PUBMED:15857680].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(60)
Full
(1535)
Representative proteomes UniProt
(2529)
NCBI
(4464)
Meta
(0)
RP15
(350)
RP35
(690)
RP55
(1116)
RP75
(1290)
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

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  Seed
(60)
Full
(1535)
Representative proteomes UniProt
(2529)
NCBI
(4464)
Meta
(0)
RP15
(350)
RP35
(690)
RP55
(1116)
RP75
(1290)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(60)
Full
(1535)
Representative proteomes UniProt
(2529)
NCBI
(4464)
Meta
(0)
RP15
(350)
RP35
(690)
RP55
(1116)
RP75
(1290)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Alignment kindly provided by SMART
Previous IDs: DAX;
Type: Family
Sequence Ontology: SO:0100021
Author: Marcu O, SMART
Number in seed: 60
Number in full: 1535
Average length of the domain: 75.30 aa
Average identity of full alignment: 39 %
Average coverage of the sequence by the domain: 12.03 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.2 22.2
Trusted cut-off 22.2 22.2
Noise cut-off 22.1 22.1
Model length: 81
Family (HMM) version: 17
Download: download the raw HMM for this family

Species distribution

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Interactions

There is 1 interaction for this family. More...

DIX

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the DIX domain has been found. There are 29 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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