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136  structures 299  species 1  interaction 1407  sequences 23  architectures

Family: Glyco_hydro_7 (PF00840)

Summary: Glycosyl hydrolase family 7

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This is the Wikipedia entry entitled "Glycoside hydrolase family 7". More...

Glycoside hydrolase family 7 Edit Wikipedia article

Glycosyl hydrolase family 7
PDB 4ovw EBI.jpg
endoglucanase i complexed with epoxybutyl cellobiose
Identifiers
Symbol Glyco_hydro_7
Pfam PF00840
Pfam clan CL0004
InterPro IPR001722
SCOP 1cel
SUPERFAMILY 1cel
CAZy GH7
CDD cd07999

In molecular biology, glycoside hydrolase family 7 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]

Glycoside hydrolase family 7 CAZY GH_7 comprises enzymes with several known activities including endoglucanase (EC 3.2.1.4) and cellobiohydrolase (EC 3.2.1.91). These enzymes were formerly known as cellulase family C.

Exoglucanases and cellobiohydrolases[8] play a role in the conversion of cellulose to glucose by cutting the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain. Structurally, cellulases and xylanases frequently consist of a catalytic domain joined to a cellulose-binding domain (CBD) via a linker region that is rich in proline and/or hydroxy-amino acids. In type I exoglucanases, the CBD domain is found at the C-terminal extremity of these enzyme (this short domain forms a hairpin loop structure stabilised by 2 disulphide bridges).

References

  1. ^ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. doi:10.1073/pnas.92.15.7090. PMC 41477Freely accessible. PMID 7624375. 
  2. ^ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779. 
  3. ^ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 ( Pt 2) (Pt 2): 695–6. PMC 1217404Freely accessible. PMID 8687420. 
  4. ^ "Home". CAZy.org. Retrieved 2018-03-06. 
  5. ^ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490–5. doi:10.1093/nar/gkt1178. PMID 24270786. 
  6. ^ "Glycoside Hydrolase Family 7". CAZypedia.org. Retrieved 2018-03-06. 
  7. ^ "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology. 28 (1): 3–8. December 2018. doi:10.1093/glycob/cwx089. PMID 29040563. 
  8. ^ Gilkes NR, Henrissat B, Kilburn DG, Miller RC, Warren RA (June 1991). "Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families". Microbiological Reviews. 55 (2): 303–15. PMC 372816Freely accessible. PMID 1886523. 

This article incorporates text from the public domain Pfam and InterPro IPR001722

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

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Literature references

  1. Sulzenbacher G, Driguez H, Henrissat B, Schulein M, Davies GJ; , Biochemistry 1996;35:15280-15287.: Structure of the Fusarium oxysporum endoglucanase I with a nonhydrolyzable substrate analogue: substrate distortion gives rise to the preferred axial orientation for the leaving group. PUBMED:8952478 EPMC:8952478


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001722

O-Glycosyl hydrolases (EC) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PUBMED:7624375, PUBMED:8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Glycoside hydrolase family 7 CAZY comprises enzymes with several known activities; endoglucanase (EC); cellobiohydrolase (EC). These enzymes were formerly known as cellulase family C.

Exoglucanases and cellobiohydrolases [PUBMED:1886523] play a role in the conversion of cellulose to glucose by cutting the dissaccharide cellobiose from the nonreducing end of the cellulose polymer chain. Structurally, cellulases and xylanases generally consist of a catalytic domain joined to a cellulose-binding domain (CBD) via a linker region that is rich in proline and/or hydroxy-amino acids. In type I exoglucanases, the CBD domain is found at the C-terminal extremity of these enzyme (this short domain forms a hairpin loop structure stabilised by 2 disulphide bridges).

Gene Ontology

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Domain organisation

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Alignments

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(127)
Full
(1407)
Representative proteomes UniProt
(7078)
NCBI
(6943)
Meta
(4)
RP15
(329)
RP35
(796)
RP55
(1089)
RP75
(1431)
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Format an alignment

  Seed
(127)
Full
(1407)
Representative proteomes UniProt
(7078)
NCBI
(6943)
Meta
(4)
RP15
(329)
RP35
(796)
RP55
(1089)
RP75
(1431)
Alignment:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(127)
Full
(1407)
Representative proteomes UniProt
(7078)
NCBI
(6943)
Meta
(4)
RP15
(329)
RP35
(796)
RP55
(1089)
RP75
(1431)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1478 (release 2.1)
Previous IDs: glycosyl_hydr13;
Type: Domain
Sequence Ontology: SO:0000417
Author: Bateman A
Number in seed: 127
Number in full: 1407
Average length of the domain: 355.40 aa
Average identity of full alignment: 47 %
Average coverage of the sequence by the domain: 85.75 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 19.6 19.6
Trusted cut-off 26.4 19.8
Noise cut-off 17.5 19.4
Model length: 434
Family (HMM) version: 20
Download: download the raw HMM for this family

Species distribution

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Interactions

There is 1 interaction for this family. More...

Glyco_hydro_7

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_7 domain has been found. There are 136 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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