Summary: Type II intron maturase
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Type II intron maturase Provide feedback
Group II introns use intron-encoded reverse transcriptase, maturase and DNA endonuclease activities for site-specific insertion into DNA . Although this type of intron is self splicing in vitro they require a maturase protein for splicing in vivo. It has been shown that a specific region of the aI2 intron is needed for the maturase function . This region was found to be conserved in group II introns and called domain X .
Moran JV, Mecklenburg KL, Sass P, Belcher SM, Mahnke D, Lewin A, Perlman P; , Nucleic Acids Res 1994;22:2057-2064.: Splicing defective mutants of the COXI gene of yeast mitochondrial DNA: initial definition of the maturase domain of the group II intron aI2. PUBMED:8029012 EPMC:8029012
Guo H, Zimmerly S, Perlman PS, Lambowitz AM; , EMBO J 1997;16:6835-6848.: Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double-stranded DNA. PUBMED:9362497 EPMC:9362497
Mohr G, Perlman PS, Lambowitz AM; , Nucleic Acids Res 1993;21:4991-4997.: Evolutionary relationships among group II intron-encoded proteins and identification of a conserved domain that may be related to maturase function. PUBMED:8255751 EPMC:8255751
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR024937
Group II introns are widespread in plant cell organelles [PUBMED:16763758]. In vivo, most plant group II introns do not self-splice, but require the assistance of proteinaceous splicing factors, known as maturases. In higher plants, maturases are encoded for in the nuclear genes [PUBMED:12527773], but are otherwise encoded by organellar introns.This entry represents a sequence region known as domain X, which is conserved in group II introns [PUBMED:8255751] and is required for maturase function [PUBMED:8029012].
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|Biological process||mRNA processing (GO:0006397)|
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|Seed source:||Pfam-B_105 (release 3.0)|
|Number in seed:||25|
|Number in full:||36524|
|Average length of the domain:||102.30 aa|
|Average identity of full alignment:||63 %|
|Average coverage of the sequence by the domain:||26.36 %|
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build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||16|
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