Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
19  structures 280  species 2  interactions 2824  sequences 22  architectures

Family: Pollen_allerg_1 (PF01357)

Summary: Pollen allergen

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Pollen allergen Provide feedback

This family contains allergens lol PI, PII and PIII from Lolium perenne.

Literature references

  1. Ansari AA, Shenbagamurthi P, Marsh DG; , Biochemistry 1989;28:8665-8670.: Complete primary structure of a Lolium perenne (perennial rye grass) pollen allergen, Lol p III: comparison with known Lol p I and II sequences. PUBMED:2605214 EPMC:2605214


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR007117

Expansins are secreted proteins of 25 to 27 Kd that were isolated first from young cucumber seedling and subsequently from other plant tissues. Expression of expansin genes correlates with growth of cells. Increase in expansin content also occurs during fruit ripening. Expansins act on the cell wall to promote its extensibility. The model for its mechanism of action postulates that expansins break non-covalent bonds between cell-wall polysaccharides, thereby permitting pressure dependent expansion of the cell [PUBMED:11014181, PUBMED:11641069].

Group-I pollen allergens of grasses have limited but significant sequence homology to expansin. These proteins are the main causative agent of hay fever and seasonal asthma induced by grass pollen. Extracts containing group-I allergens are also active in loosening cell-walls. Group-I pollen allergens and related proteins in vegetative tissues have been classified as beta-expansins, whereas the earlier discovered expansins are now referred to as alpha-expansins [PUBMED:9177257].

Expansin-like proteins are also found in some fungi. In Trichoderma reesei an expansin-like protein (Cel12A) acts as a glycoside hydrolase on xyloglucan and 1-4 beta-glucan. These hydrolytic actions differ from the action by expansins, which induce wall extension by a non-hydrolytic mechanism [PUBMED:11553760].

Expansins consist of two domains closely packed and aligned so as to form a long, shallow groove with potential to bind a glycan backbone of ~10 sugar residues. The N-terminal cysteine-rich domain has distant sequence similarity to family-45 endoglucanases (EG45-like domain). The ~90- residue C-terminal domain may function as a cellulose-binding domain (CBD). It is composed of eight beta-strands assembled into two antiparallel beta-sheets. The two beta-sheets are at slight angles to each other and form a beta- sandwich similar to the Ig fold [PUBMED:16984999].

This entry represents the expansin C-terminal CBD-like domain.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(346)
Full
(2824)
Representative proteomes NCBI
(2676)
Meta
(0)
RP15
(209)
RP35
(855)
RP55
(1305)
RP75
(1353)
Jalview View  View  View  View  View  View  View   
HTML View  View  View  View  View  View     
PP/heatmap 1 View  View  View  View  View     
Pfam viewer View  View             

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(346)
Full
(2824)
Representative proteomes NCBI
(2676)
Meta
(0)
RP15
(209)
RP35
(855)
RP55
(1305)
RP75
(1353)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(346)
Full
(2824)
Representative proteomes NCBI
(2676)
Meta
(0)
RP15
(209)
RP35
(855)
RP55
(1305)
RP75
(1353)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: SCOP
Previous IDs: Pollen_allergen;
Type: Domain
Author: Bateman A
Number in seed: 346
Number in full: 2824
Average length of the domain: 77.60 aa
Average identity of full alignment: 38 %
Average coverage of the sequence by the domain: 32.25 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 80369284 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.7 20.7
Trusted cut-off 20.7 20.7
Noise cut-off 20.6 20.6
Model length: 80
Family (HMM) version: 17
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Show

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Interactions

There are 2 interactions for this family. More...

Pollen_allerg_1 DPBB_1

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Pollen_allerg_1 domain has been found. There are 19 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

Loading structure mapping...