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1  structure 785  species 0  interactions 3585  sequences 98  architectures

Family: Peptidase_M8 (PF01457)

Summary: Leishmanolysin

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Leishmanolysin Provide feedback

No Pfam abstract.

Literature references

  1. Rawlings ND, Barrett AJ; , Meth Enzymol 1995;248:183-228.: Evolutionary families of metallopeptidases. PUBMED:7674922 EPMC:7674922


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001577

This group of metallopeptidases belong to the MEROPS peptidase family M8 (leishmanolysin family, clan MA(M)). The protein fold of the peptidase domain for members of this family resembles that of thermolysin, the type example for clan MA.

Leishmanolysin ( EC ) is an enzyme found in eukaryotes including Leishmania and related parasitic protozoa [ PUBMED:7674922 ]. The endopeptidase is the most abundant protein on the cell surface during the promastigote stage of the parasite, and is attached to the membrane by a glycosylphosphatidylinositol anchor [ PUBMED:7674922 ]. In the amastigote form, the parasite lives in lysosomes of host macrophages, producing a form of the protease that has an acidic pH optimum [ PUBMED:7674922 ]. This differs from most other metalloproteases and may be an adaptation to the environment in which the organism survives [ PUBMED:7674922 ].

Homologues are known from human (leishmanolysin-like peptidase or invadolysin [ PUBMED:19706689 ] and leishmanolysin-like peptidase 2) and other chordates, but have not been well-characterized

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Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(8)
Full
(3585)
Representative proteomes UniProt
(6447)
RP15
(947)
RP35
(1676)
RP55
(2770)
RP75
(3776)
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(8)
Full
(3585)
Representative proteomes UniProt
(6447)
RP15
(947)
RP35
(1676)
RP55
(2770)
RP75
(3776)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(8)
Full
(3585)
Representative proteomes UniProt
(6447)
RP15
(947)
RP35
(1676)
RP55
(2770)
RP75
(3776)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Prodom_3085 (release 99.1)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A
Number in seed: 8
Number in full: 3585
Average length of the domain: 299.6 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 61.45 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild --amino -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 21.0 21.0
Trusted cut-off 21.0 21.0
Noise cut-off 20.9 20.9
Model length: 529
Family (HMM) version: 19
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
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Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Peptidase_M8 domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044RS49 View 3D Structure Click here
A0A077YZZ2 View 3D Structure Click here
A0A077Z5E1 View 3D Structure Click here
A0A077Z9U2 View 3D Structure Click here
A0A0G2JX27 View 3D Structure Click here
A0A0K0E8V4 View 3D Structure Click here
A0A0K0EGQ2 View 3D Structure Click here
A0A0R0JBF1 View 3D Structure Click here
A0A1B0GTW7 View 3D Structure Click here
A0A1D6L355 View 3D Structure Click here
A0A286YEC0 View 3D Structure Click here
A0A2K6WJ30 View 3D Structure Click here
A0A3P7DUR1 View 3D Structure Click here
A0A3P7E193 View 3D Structure Click here
A0A3Q0KIZ7 View 3D Structure Click here
A0A3Q0KJ06 View 3D Structure Click here
A0A3Q0KL52 View 3D Structure Click here
A0A3Q0KQB5 View 3D Structure Click here
A0A3Q0KS51 View 3D Structure Click here
A0A5K4EV09 View 3D Structure Click here
A0A5K4F1I9 View 3D Structure Click here
A0A5K4F3D9 View 3D Structure Click here
A0A5K4F4I0 View 3D Structure Click here
A0A5K4F5F3 View 3D Structure Click here
A0A5K4F5F5 View 3D Structure Click here
A0A5K4F5V4 View 3D Structure Click here
A0A5K4F759 View 3D Structure Click here
A0A5K4F8T6 View 3D Structure Click here
A0A5K4F9K0 View 3D Structure Click here
A0A5K4F9K7 View 3D Structure Click here
A0A5K4FAH1 View 3D Structure Click here
A0A5K4FAZ3 View 3D Structure Click here
A0A5K4FBU3 View 3D Structure Click here
A0A5K4FD50 View 3D Structure Click here
A0A5S6PSP5 View 3D Structure Click here
A0A5S6PT09 View 3D Structure Click here
A4HUF6 View 3D Structure Click here
A4HUF8 View 3D Structure Click here
A4HUF9 View 3D Structure Click here
A4HUG0 View 3D Structure Click here