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81  structures 3902  species 1  interaction 9582  sequences 134  architectures

Family: CorA (PF01544)

Summary: CorA-like Mg2+ transporter protein

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This is the Wikipedia entry entitled "CorA metal ion transporter". More...

CorA metal ion transporter Edit Wikipedia article

CorA-like Mg2+ transporter protein
PDB 2bbj EBI.jpg
Structure of the CorA Mg2+ transporter.[1]
Identifiers
Symbol CorA
Pfam PF01544
InterPro IPR002523
TCDB 1.A.35
OPM superfamily 72
OPM protein 2bbj
CDD cd11744

The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli.[2][3] CorA is ubiquitous in the Bacteria and Archaea. There are also eukaryotic members of the family.

Subfamilies

Human proteins containing this domain

MRS2L;

References

  1. ^ Lunin VV, Dobrovetsky E, Khutoreskaya G, et al. (April 2006). "Crystal structure of the CorA Mg2+ transporter". Nature. 440 (7085): 833–7. doi:10.1038/nature04642. PMID 16598263. 
  2. ^ Maguire ME, Kehres DG, Lawyer CH (1998). "The CorA magnesium transporter gene family". Microb. Comp. Genomics. 3 (3): 151–169. doi:10.1089/omi.1.1998.3.151. PMID 9775386. 
  3. ^ Smith RL, Maguire ME, Szegedy MA, Kucharski LM, Walker C, Wiet RM, Redpath A, Kaczmarek MT (1998). "The CorA Mg2+ transport protein of Salmonella typhimurium. Mutagenesis of conserved residues in the third membrane domain identifies a Mg2+ pore". J. Biol. Chem. 273 (44): 28663–28669. doi:10.1074/jbc.273.44.28663. PMID 9786860. 

Further reading

  • Kehres DG, Lawyer CH, Maguire ME (1998). "The CorA magnesium transporter gene family". Microb. Comp. Genomics. 3 (3): 151–69. doi:10.1089/omi.1.1998.3.151. PMID 9775386. 
  • Smith RL, Szegedy MA, Kucharski LM, et al. (October 1998). "The CorA Mg2+ transport protein of Salmonella typhimurium. Mutagenesis of conserved residues in the third membrane domain identifies a Mg2+ pore". J. Biol. Chem. 273 (44): 28663–9. doi:10.1074/jbc.273.44.28663. PMID 9786860. 
  • Wiesenberger G, Waldherr M, Schweyen RJ (April 1992). "The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo". J. Biol. Chem. 267 (10): 6963–9. PMID 1551905. 

This article incorporates text from the public domain Pfam and InterPro IPR002523


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

CorA-like Mg2+ transporter protein Provide feedback

The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein Q01926 from yeast that is thought to be an RNA splicing protein [3]. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.

Literature references

  1. Kehres DG, Lawyer CH, Maguire ME; , Microb Comp Genomics 1998;3:151-169.: The CorA magnesium transporter gene family. PUBMED:9775386 EPMC:9775386

  2. Smith RL, Szegedy MA, Kucharski LM, Walker C, Wiet RM, Redpath A, Kaczmarek MT, Maguire ME; , J Biol Chem 1998;273:28663-28669.: The CorA Mg2+ transport protein of Salmonella typhimurium. Mutagenesis of conserved residues in the third membrane domain identifies a Mg2+ pore. PUBMED:9786860 EPMC:9786860

  3. Wiesenberger G, Waldherr M, Schweyen RJ; , J Biol Chem 1992;267:6963-6969.: The nuclear gene MRS2 is essential for the excision of group II introns from yeast mitochondrial transcripts in vivo. PUBMED:1551905 EPMC:1551905


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR002523

This entry includes prokaryotic magnesium transport protein CorA and its related protein, zinc transport protein ZntB [PUBMED:12142406]. This entry also includes eukaryotic magnesium transporters, such as mitochondrial inner membrane magnesium transporter Mrs2 and magnesium transporter Alr1 and Alr2. These proteins are characterised by the conserved GMN motif at the end of the first of two conserved transmembrane (TM) domains near the C terminus [PUBMED:17619822].

Thermotoga maritima CorA (TmCorA) has been reported to be an efflux system. It only has 2 TM domains,TM1 and TM2 [PUBMED:16857941, PUBMED:12206392, PUBMED:16857941]. The loop connecting TM1 and TM2 contains the conserved CorA signature motifs YGMNF and MPEL. With its N- and C-terminal ends face the cytosol and its similarity to the class II CorA (a CorA group lacking the MPEL motif and may transport divalent cations out of the cell), TmCorA is predicted to be primarily involved in ion efflux [PUBMED:16857941]. It forms a pentameric membrane protein channel featuring a possible ion discriminating aspartate ring at the cytoplasmic entrance of the pore and two distinct cytoplasmic metal binding sites per monomer, which could have regulatory roles [PUBMED:16857941, PUBMED:23425532].

It has been suggested that eukaryotic CorA homologues have the same topology and overall structure as T. maritima CorA [PUBMED:17619822]. In budding yeasts, Mrs2 is an essential component of the major electrophoretic Mg2+ influx system in mitochondria [PUBMED:12628916]. Overexpression of Alr1 or Alr2 increases tolerance to Al3+ and Ga3+ ions [PUBMED:9430719]. Despite generally low sequence similarity, both human Mrs2 (also known as LPE10) and S. typhimurium CorA can restore Mg2+ uptake activity in Saccharomyces cerevisiae with inactive Mrs2 [PUBMED:17619822].

Gene Ontology

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Domain organisation

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(58)
Full
(9582)
Representative proteomes UniProt
(24656)
NCBI
(34387)
Meta
(398)
RP15
(1575)
RP35
(5026)
RP55
(8671)
RP75
(13660)
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Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(58)
Full
(9582)
Representative proteomes UniProt
(24656)
NCBI
(34387)
Meta
(398)
RP15
(1575)
RP35
(5026)
RP55
(8671)
RP75
(13660)
Alignment:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(58)
Full
(9582)
Representative proteomes UniProt
(24656)
NCBI
(34387)
Meta
(398)
RP15
(1575)
RP35
(5026)
RP55
(8671)
RP75
(13660)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_944 (release 4.0) & Pfam-B_3206 (release 7.5)
Previous IDs: none
Type: Family
Author: Bateman A
Number in seed: 58
Number in full: 9582
Average length of the domain: 254.00 aa
Average identity of full alignment: 16 %
Average coverage of the sequence by the domain: 55.95 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.0
Noise cut-off 26.9 26.9
Model length: 292
Family (HMM) version: 17
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
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Viroids Viroids Unclassified sequence Unclassified sequence

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Interactions

There is 1 interaction for this family. More...

CorA

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the CorA domain has been found. There are 81 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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