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35  structures 1489  species 0  interactions 8422  sequences 114  architectures

# Summary: 6-phosphofructo-2-kinase

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "6-phosphofructo-2-kinase". More...

# 6-phosphofructo-2-kinase

In enzymology, a 6-phosphofructo-2-kinase (EC 2.7.1.105) is an enzyme that catalyzes the chemical reaction

ATP + beta-D-fructose 6-phosphate ${\displaystyle \rightleftharpoons }$ ADP + beta-D-fructose 2,6-bisphosphate

Thus, the two substrates of this enzyme are ATP and beta-D-fructose 6-phosphate, whereas its two products are ADP and beta-D-fructose 2,6-bisphosphate.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:beta-D-fructose-6-phosphate 2-phosphotransferase. Other names in common use include phosphofructokinase 2, 6-phosphofructose 2-kinase, 6-phosphofructo-2-kinase (phosphorylating), fructose 6-phosphate 2-kinase, and ATP:D-fructose-6-phosphate 2-phosphotransferase. This enzyme participates in fructose and mannose metabolism.

## Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1BIF, 1K6M, 2AXN, 2BIF, 2DWO, 2DWP, 2I1V, and 3BIF.

## References

• Van Schaftingen E, Hers HG (1981). "Phosphofructokinase 2: the enzyme that forms fructose 2,6-bisphosphate from fructose 6-phosphate and ATP". Biochem. Biophys. Res. Commun. 101: 1078â€“84. PMIDÂ 6458291.

This is the Wikipedia entry entitled "Phosphofructokinase 2". More...

# Phosphofructokinase 2

converts fructose 6-phosphate to fructose 2,6-bisphosphate

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

# 6-phosphofructo-2-kinase

This enzyme occurs as a bifunctional enzyme with fructose-2,6-bisphosphatase. The bifunctional enzyme catalyses both the synthesis and degradation of fructose-2,6-bisphosphate, a potent regulator of glycolysis [1]. This enzyme contains a P-loop motif.

## Literature references

1. Hasemann CA, Istvan ES, Uyeda K, Deisenhofer J; , Structure 1996;4:1017-1029.: The crystal structure of the bifunctional enzyme 6-phosphofructo-2- kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies. PUBMED:8805587 EPMC:8805587

This tab holds annotation information from the InterPro database.

# InterPro entry IPR013079

6-Phosphofructo-2-kinase ( EC , EC ) is a bifunctional enzyme that catalyses both the synthesis and the degradation of fructose-2, 6-bisphosphate. The fructose-2,6-bisphosphatase reaction involves a phosphohistidine intermediate. The catalytic pathway is: ATP + D-fructose 6-phosphate = ADP + D-fructose 2,6-bisphosphate D-fructose 2,6-bisphosphate + H 2 O = 6-fructose 6-phosphate + P i The enzyme is important in the regulation of hepatic carbohydrate metabolism and is found in greatest quantities in the liver, kidney and heart. In mammals, several genes often encode different isoforms, each of which differs in its tissue distribution and enzymatic activity [ PUBMED:9652401 ]. The family described here bears a resemblance to the ATP-driven phospho-fructokinases, however, they share little sequence similarity, although a few residues seem key to their interaction with fructose 6-phosphate [ PUBMED:9753654 ].

This domain forms the N-terminal region of this enzyme, while INTERPRO forms the C-terminal domain.

### Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

# Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

# Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

## View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Seed
(11)
Full
(8422)
Representative proteomes UniProt
(12654)
RP15
(1084)
RP35
(2740)
RP55
(5996)
RP75
(8493)
Jalview View  View  View  View  View  View  View
HTML View
PP/heatmap 1

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: available, not generated, not available.

## Format an alignment

Seed
(11)
Full
(8422)
Representative proteomes UniProt
(12654)
RP15
(1084)
RP35
(2740)
RP55
(5996)
RP75
(8493)
Alignment:
Format:
Order:
Sequence:
Gaps:

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Seed
(11)
Full
(8422)
Representative proteomes UniProt
(12654)
RP15
(1084)
RP35
(2740)
RP55
(5996)
RP75
(8493)

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

# HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

# Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

# Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

## Curation

 Seed source: Pfam-B_717 (release 4.1) Previous IDs: none Type: Domain Sequence Ontology: SO:0000417 Author: Bateman A Number in seed: 11 Number in full: 8422 Average length of the domain: 196.6 aa Average identity of full alignment: 44 % Average coverage of the sequence by the domain: 41.04 %

## HMM information

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 27.0
Trusted cut-off 27.0 27.1
Noise cut-off 26.9 26.9
Model length: 223
Family (HMM) version: 21

# Species distribution

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### Colour assignments

 Archea Eukaryota Bacteria Other sequences Viruses Unclassified Viroids Unclassified sequence

### Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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# Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the 6PF2K domain has been found. There are 35 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

# AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information