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10  structures 292  species 0  interactions 1081  sequences 15  architectures

Family: B56 (PF01603)

Summary: Protein phosphatase 2A regulatory B subunit (B56 family)

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This is the Wikipedia entry entitled "B56 domain". More...

B56 domain Edit Wikipedia article

B56
PDB 2nyl EBI.jpg
crystal structure of protein phosphatase 2a (pp2a) holoenzyme with the catalytic subunit carboxyl terminus truncated
Identifiers
Symbol B56
Pfam PF01603
InterPro IPR002554
SCOP 1qq3
SUPERFAMILY 1qq3
TCDB 5.A.4

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism. Phosphorylation enables the activation or The ability of this widely distributed heterotrimeric enzyme to act on a diverse array of substrates is largely controlled by the nature of its regulatory B subunit. There are multiple families of B subunits, this family is called the B56 family.[1]

Function[edit]

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism such as DNA replication, transcription, translation, cell cycle, development, and apoptosis.

In order to exhibit specificity control over catalysis, hetero-oligomers are formed so the catalytic subunits are complexed with regulatory subunits. This helps direct their action towards the cellular substrate. This is the function of the B56 domain.[2]

B56 alpha, is additionally thought to play a role in nuclear export.[3]

Structure[edit]

Regulatory B subunit is a heterotrimer.

Subcellular Localization[edit]

Mainly found in the cytoplasm, however various isoforms can be found in the nucleus. [3]

Isoforms of B56 protein family[edit]

  • B56alpha
  • B56beta
  • B56delta
  • B56epsilon
  • B56gamma[3]

References[edit]

  1. ^ McCright B, Virshup DM (November 1995). "Identification of a new family of protein phosphatase 2A regulatory subunits". J. Biol. Chem. 270 (44): 26123–8. doi:10.1074/jbc.270.44.26123. PMID 7592815. 
  2. ^ Magnusdottir A, Stenmark P, Flodin S, Nyman T, Kotenyova T, Gräslund S et al. (2009). "The structure of the PP2A regulatory subunit B56 gamma: the remaining piece of the PP2A jigsaw puzzle.". Proteins 74 (1): 212–21. doi:10.1002/prot.22150. PMID 18618707. 
  3. ^ a b c Flegg CP, Sharma M, Medina-Palazon C, Jamieson C, Galea M, Brocardo MG et al. (2010). "Nuclear export and centrosome targeting of the protein phosphatase 2A subunit B56alpha: role of B56alpha in nuclear export of the catalytic subunit.". J Biol Chem 285 (24): 18144–54. doi:10.1074/jbc.M109.093294. PMC 2881738. PMID 20378546. 

This article incorporates text from the public domain Pfam and InterPro IPR002554

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Protein phosphatase 2A regulatory B subunit (B56 family) Provide feedback

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism. The ability of this widely distributed heterotrimeric enzyme to act on a diverse array of substrates is largely controlled by the nature of its regulatory B subunit. There are multiple families of B subunits (See also PF01240), this family is called the B56 family [1].

Literature references

  1. McCright B, Virshup DM; , J Biol Chem 1995;270:26123-26128.: Identification of a new family of protein phosphatase 2A regulatory subunits. PUBMED:7592815 EPMC:7592815


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR002554

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism. The ability of this widely distributed heterotrimeric enzyme to act on a diverse array of substrates is largely controlled by the nature of its regulatory B subunit. There are multiple families of B subunits, this family is called the B56 family [PUBMED:7592815].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(54)
Full
(1081)
Representative proteomes NCBI
(990)
Meta
(15)
RP15
(209)
RP35
(349)
RP55
(494)
RP75
(639)
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Format an alignment

  Seed
(54)
Full
(1081)
Representative proteomes NCBI
(990)
Meta
(15)
RP15
(209)
RP35
(349)
RP55
(494)
RP75
(639)
Alignment:
Format:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(54)
Full
(1081)
Representative proteomes NCBI
(990)
Meta
(15)
RP15
(209)
RP35
(349)
RP55
(494)
RP75
(639)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_984 (release 4.1)
Previous IDs: none
Type: Family
Author: Bateman A
Number in seed: 54
Number in full: 1081
Average length of the domain: 337.90 aa
Average identity of full alignment: 49 %
Average coverage of the sequence by the domain: 73.42 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 25.4 25.1
Noise cut-off 23.4 24.4
Model length: 409
Family (HMM) version: 15
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the B56 domain has been found. There are 10 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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