Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
23  structures 1406  species 0  interactions 6797  sequences 93  architectures

Family: B56 (PF01603)

Summary: Protein phosphatase 2A regulatory B subunit (B56 family)

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "B56 domain". More...

B56 domain Edit Wikipedia article

B56
PDB 2nyl EBI.jpg
crystal structure of protein phosphatase 2a (pp2a) holoenzyme with the catalytic subunit carboxyl terminus truncated
Identifiers
Symbol B56
Pfam PF01603
InterPro IPR002554
SCOP 1qq3
SUPERFAMILY 1qq3
TCDB 5.A.4

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism. Phosphorylation enables the activation or The ability of this widely distributed heterotrimeric enzyme to act on a diverse array of substrates is largely controlled by the nature of its regulatory B subunit. There are multiple families of B subunits, this family is called the B56 family.[1]

Function

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism such as DNA replication, transcription, translation, cell cycle, development, and apoptosis.

In order to exhibit specificity control over catalysis, hetero-oligomers are formed so the catalytic subunits are complexed with regulatory subunits. This helps direct their action towards the cellular substrate. This is the function of the B56 domain.[2]

B56 alpha, is additionally thought to play a role in nuclear export.[3]

Structure

Regulatory B subunit is a heterotrimer.

Subcellular Localization

Mainly found in the cytoplasm, however various isoforms can be found in the nucleus.[3]

Isoforms of B56 protein family

  • B56alpha
  • B56beta
  • B56delta
  • B56epsilon
  • B56gamma[3]

References

  1. ^ McCright B, Virshup DM (November 1995). "Identification of a new family of protein phosphatase 2A regulatory subunits". J. Biol. Chem. 270 (44): 26123–8. PMID 7592815. doi:10.1074/jbc.270.44.26123. 
  2. ^ Magnusdottir A, Stenmark P, Flodin S, Nyman T, Kotenyova T, Gräslund S, et al. (2009). "The structure of the PP2A regulatory subunit B56 gamma: the remaining piece of the PP2A jigsaw puzzle.". Proteins. 74 (1): 212–21. PMID 18618707. doi:10.1002/prot.22150. 
  3. ^ a b c Flegg CP, Sharma M, Medina-Palazon C, Jamieson C, Galea M, Brocardo MG, et al. (2010). "Nuclear export and centrosome targeting of the protein phosphatase 2A subunit B56alpha: role of B56alpha in nuclear export of the catalytic subunit.". J Biol Chem. 285 (24): 18144–54. PMC 2881738Freely accessible. PMID 20378546. doi:10.1074/jbc.M109.093294. 

This article incorporates text from the public domain Pfam and InterPro IPR002554

External links

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Protein phosphatase 2A regulatory B subunit (B56 family) Provide feedback

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism. The ability of this widely distributed heterotrimeric enzyme to act on a diverse array of substrates is largely controlled by the nature of its regulatory B subunit. There are multiple families of B subunits (See also PF01240), this family is called the B56 family [1].

Literature references

  1. McCright B, Virshup DM; , J Biol Chem 1995;270:26123-26128.: Identification of a new family of protein phosphatase 2A regulatory subunits. PUBMED:7592815 EPMC:7592815


This tab holds annotation information from the InterPro database.

InterPro entry IPR002554

Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism. The ability of this widely distributed heterotrimeric enzyme to act on a diverse array of substrates is largely controlled by the nature of its regulatory B subunit. There are multiple families of B subunits, this family is called the B56 family [ PUBMED:7592815 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Pfam Clan

This family is a member of clan TPR (CL0020), which has the following description:

Tetratricopeptide-like repeats are found in a numerous and diverse proteins involved in such functions as cell cycle regulation, transcriptional control, mitochondrial and peroxisomal protein transport, neurogenesis and protein folding.

The clan contains the following 176 members:

Adaptin_N Alkyl_sulf_dimr ANAPC3 ANAPC5 ANAPC8 APC_rep API5 Arm Arm_2 Arm_3 Arm_vescicular Atx10homo_assoc B56 BAF250_C BTAD CAS_CSE1 ChAPs CHIP_TPR_N CID CLASP_N Clathrin Clathrin-link Clathrin_H_link Clathrin_propel Cnd1 Cnd3 Coatomer_E Cohesin_HEAT Cohesin_load ComR_TPR COPI_C CPL CRM1_C CRM1_repeat CRM1_repeat_3 Cse1 CTK3 DHR-2 DIL DNA_alkylation Dopey_N Drf_FH3 Drf_GBD DUF1822 DUF2019 DUF2225 DUF3385 DUF3458_C DUF3808 DUF3856 DUF4042 DUF5588 DUF5691 DUF6340 DUF6377 DUF924 EAD11 EST1 EST1_DNA_bind FAT Fis1_TPR_C Fis1_TPR_N Foie-gras_1 GUN4_N HAT HEAT HEAT_2 HEAT_EZ HEAT_PBS HEAT_UF HemY_N HrpB1_HrpK HSM3_C HSM3_N IBB IBN_N IFRD Importin_rep Importin_rep_2 Importin_rep_3 Importin_rep_4 Importin_rep_5 Importin_rep_6 Insc_C KAP Leuk-A4-hydro_C LRV LRV_FeS MA3 MIF4G MIF4G_like MIF4G_like_2 MMS19_C Mo25 MRP-S27 Mtf2 NARP1 Neurochondrin Nipped-B_C Nro1 NSF Paf67 ParcG PC_rep PHAT PI3Ka PknG_TPR PPP5 PPR PPR_1 PPR_2 PPR_3 PPR_long PPTA Proteasom_PSMB PUF Rapsyn_N RIX1 RNPP_C RPM2 RPN7 Sel1 SHNi-TPR SNAP SPO22 SRP_TPR_like ST7 Suf SusD-like SusD-like_2 SusD-like_3 SusD_RagB SYCP2_ARLD TAF6_C TAL_effector TAtT Tcf25 TIP120 TOM20_plant TPR_1 TPR_10 TPR_11 TPR_12 TPR_14 TPR_15 TPR_16 TPR_17 TPR_18 TPR_19 TPR_2 TPR_20 TPR_21 TPR_22 TPR_3 TPR_4 TPR_5 TPR_6 TPR_7 TPR_8 TPR_9 TPR_MalT TTC7_N UNC45-central Upf2 V-ATPase_H_C V-ATPase_H_N Vac14_Fab1_bd Vitellogenin_N Vps39_1 W2 WSLR Wzy_C_2 Xpo1 YcaO_C YfiO Zmiz1_N

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(107)
Full
(6797)
Representative proteomes UniProt
(11632)
RP15
(1115)
RP35
(3125)
RP55
(5625)
RP75
(7313)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(107)
Full
(6797)
Representative proteomes UniProt
(11632)
RP15
(1115)
RP35
(3125)
RP55
(5625)
RP75
(7313)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(107)
Full
(6797)
Representative proteomes UniProt
(11632)
RP15
(1115)
RP35
(3125)
RP55
(5625)
RP75
(7313)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_984 (release 4.1)
Previous IDs: none
Type: Repeat
Sequence Ontology: SO:0001068
Author: Bateman A
Number in seed: 107
Number in full: 6797
Average length of the domain: 347.20 aa
Average identity of full alignment: 53 %
Average coverage of the sequence by the domain: 72.91 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 25.0 25.1
Noise cut-off 24.7 24.9
Model length: 413
Family (HMM) version: 22
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Hide

Weight segments by...


Change the size of the sunburst

Small
Large

Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence

Selections

Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the B56 domain has been found. There are 23 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2JTA1 View 3D Structure Click here
A0A0G2JZD1 View 3D Structure Click here
A0A0R0EB29 View 3D Structure Click here
A0A1D6E7Z2 View 3D Structure Click here
A0A1D6ENF4 View 3D Structure Click here
A0A1D6EU69 View 3D Structure Click here
A0A1D6FJS2 View 3D Structure Click here
A0A1D6KYH2 View 3D Structure Click here
A0A1D6KYH2 View 3D Structure Click here
A0A1D6LBN4 View 3D Structure Click here
A0A1D6LEP6 View 3D Structure Click here
A0A1D6LZH5 View 3D Structure Click here
A0A1D6NC24 View 3D Structure Click here
A0A1D6PV45 View 3D Structure Click here
A0A2R8QI80 View 3D Structure Click here
A0A6F7R657 View 3D Structure Click here
A4QP33 View 3D Structure Click here
A9UJN4 View 3D Structure Click here
B4FXJ3 View 3D Structure Click here
B7ZXD0 View 3D Structure Click here
B7ZZN9 View 3D Structure Click here
C0P4C8 View 3D Structure Click here
C0PBF4 View 3D Structure Click here
C4J4T0 View 3D Structure Click here
D3ZDI7 View 3D Structure Click here
F1MAA3 View 3D Structure Click here
F1Q9Y3 View 3D Structure Click here
F1QSR1 View 3D Structure Click here
I1JIL3 View 3D Structure Click here
I1JPR3 View 3D Structure Click here
I1JUI3 View 3D Structure Click here
I1K137 View 3D Structure Click here
I1K1B8 View 3D Structure Click here
I1K903 View 3D Structure Click here
I1L910 View 3D Structure Click here
I1LEZ5 View 3D Structure Click here
I1LF64 View 3D Structure Click here
I1LNC5 View 3D Structure Click here
I1M7F5 View 3D Structure Click here
I1M9M6 View 3D Structure Click here
I1MUE7 View 3D Structure Click here
I1MWM0 View 3D Structure Click here
I1MYJ6 View 3D Structure Click here
I1N7G3 View 3D Structure Click here
I1NAC5 View 3D Structure Click here
K7LZS7 View 3D Structure Click here
K7MX94 View 3D Structure Click here
O04375 View 3D Structure Click here
O04376 View 3D Structure Click here
O18178 View 3D Structure Click here
P38903 View 3D Structure Click here
P78759 View 3D Structure Click here
Q0JQ73 View 3D Structure Click here
Q10428 View 3D Structure Click here
Q13362 View 3D Structure Click here
Q14738 View 3D Structure Click here
Q15172 View 3D Structure Click here
Q15173 View 3D Structure Click here
Q16537 View 3D Structure Click here
Q54VB6 View 3D Structure Click here
Q59Q44 View 3D Structure Click here
Q59Q44 View 3D Structure Click here
Q5RHP3 View 3D Structure Click here
Q60996 View 3D Structure Click here
Q61151 View 3D Structure Click here
Q6AT11 View 3D Structure Click here
Q6I621 View 3D Structure Click here
Q6PD03 View 3D Structure Click here
Q6PD28 View 3D Structure Click here
Q6YX33 View 3D Structure Click here
Q6YXJ6 View 3D Structure Click here
Q75LC7 View 3D Structure Click here
Q7FAG9 View 3D Structure Click here
Q7TNL5 View 3D Structure Click here
Q7XJ10 View 3D Structure Click here
Q7Y011 View 3D Structure Click here
Q7ZUQ2 View 3D Structure Click here
Q80W83 View 3D Structure Click here
Q8AXX6 View 3D Structure Click here
Q8IN89 View 3D Structure Click here
Q8LF36 View 3D Structure Click here
Q8RW96 View 3D Structure Click here
Q93YV6 View 3D Structure Click here
Q9LU89 View 3D Structure Click here
Q9LVE2 View 3D Structure Click here
Q9SV41 View 3D Structure Click here
Q9VXB7 View 3D Structure Click here
Q9ZQY6 View 3D Structure Click here