Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
6  structures 2656  species 1  interaction 12598  sequences 50  architectures

Family: DDE_Tnp_1 (PF01609)

Summary: Transposase DDE domain

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Transposase DDE domain Provide feedback

Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction [3]. This family contains transposases for IS4 P03835 [1] IS421 P11901 [2] IS5377 Q45620 IS427 [4] IS402 [5] IS1355 O69604 IS5, which was original isolated in bacteriophage lambda [6].

Literature references

  1. Klaer R, Kuhn S, Tillmann E, Fritz HJ, Starlinger P; , Mol Gen Genet 1981;181:169-175.: The sequence of IS4. PUBMED:6268937 EPMC:6268937

  2. Sato S, Nakada Y, Shiratsuchi A; , FEBS Lett 1989;249:21-26.: IS421, a new insertion sequence in Escherichia coli. PUBMED:2542093 EPMC:2542093

  3. DasSarma S; , Plasmid 1993;29:1-9.: Identification and analysis of the gas vesicle gene cluster on an unstable plasmid of Halobacterium halobium. PUBMED:8335077 EPMC:8335077

  4. Davies DR, Braam LM, Reznikoff WS, Rayment I; , J Biol Chem 1999;274:11904-11913.: The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution. PUBMED:10207011 EPMC:10207011

  5. De Meirsman C, Van Soom C, Verreth C, Van Gool A, Vanderleyden J; , Plasmid 1990;24:227-234.: Nucleotide sequence analysis of IS427 and its target sites in Agrobacterium tumefaciens T37. PUBMED:1963949 EPMC:1963949

  6. Ferrante AA, Lessie TG; , Gene 1991;102:143-144.: Nucleotide sequence of IS402 from Pseudomonas cepacia. PUBMED:1650732 EPMC:1650732

  7. Kroger M, Hobom G; , Nature 1982;297:159-162.: Structural analysis of insertion sequence IS5. PUBMED:6281654 EPMC:6281654


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR002559

Autonomous mobile genetic elements such as transposon or insertion sequences (IS) encode an enzyme, transposase, that is required for excising and inserting the mobile element. Transposases have been grouped into various families [PUBMED:8041625, PUBMED:1310791, PUBMED:1718819]. This family includes the IS4 transposase.

More information about these proteins can be found at Protein of the Month: Transposase [PUBMED:].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(91)
Full
(12598)
Representative proteomes NCBI
(18569)
Meta
(2527)
RP15
(850)
RP35
(1859)
RP55
(2290)
RP75
(2783)
Jalview View  View  View  View  View  View  View  View 
HTML View    View  View  View  View     
PP/heatmap 1   View  View  View  View     
Pfam viewer View  View             

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(91)
Full
(12598)
Representative proteomes NCBI
(18569)
Meta
(2527)
RP15
(850)
RP35
(1859)
RP55
(2290)
RP75
(2783)
Alignment:
Format:
Order:
Sequence:
Gaps:
Download/view:

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(91)
Full
(12598)
Representative proteomes NCBI
(18569)
Meta
(2527)
RP15
(850)
RP35
(1859)
RP55
(2290)
RP75
(2783)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1013 (release 4.1)
Previous IDs: Transposase_11;
Type: Domain
Author: Bateman A
Number in seed: 91
Number in full: 12598
Average length of the domain: 188.80 aa
Average identity of full alignment: 13 %
Average coverage of the sequence by the domain: 58.95 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.0 23.0
Trusted cut-off 23.0 23.0
Noise cut-off 22.9 22.9
Model length: 213
Family (HMM) version: 16
Download: download the raw HMM for this family

Species distribution

Sunburst controls

Show

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls

Hide

The tree shows the occurrence of this domain across different species. More...

Loading...

Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.

Interactions

There is 1 interaction for this family. More...

Dimer_Tnp_Tn5

Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the DDE_Tnp_1 domain has been found. There are 6 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

Loading structure mapping...