Summary: Choline/ethanolamine kinase
Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.
This is the Wikipedia entry entitled "Choline/ethanolamine kinase family". More...
Choline/ethanolamine kinase family Edit Wikipedia article
Choline_kinase | |||||||||
---|---|---|---|---|---|---|---|---|---|
![]() crystal structure of choline kinase
|
|||||||||
Identifiers | |||||||||
Symbol | Choline_kinase | ||||||||
Pfam | PF01633 | ||||||||
Pfam clan | CL0016 | ||||||||
InterPro | IPR002573 | ||||||||
|
In molecular biology, the choline/ethanolamine kinase family includes choline kinase(EC 2.7.1.32) and ethanolamine kinase (EC 2.7.1.82).
Ethanolamine and choline are major membrane phospholipids, in the form of glycerophosphoethanolamine and glycerophosphocholine. Ethanolamine is also a component of the glycosylphosphatidylinositol (GPI) anchor, which is necessary for cell-surface protein attachment.[1] The de novo synthesis of these phospholipids begins with the creation of phosphoethanolamine and phosphocholine by ethanolamine and choline kinases in the first step of the CDP-ethanolamine pathway.[2][3] There are two putative choline/ethanolamine kinases (C/EKs) in the Trypanosoma brucei genome.
Ethanolamine kinase has no choline kinase activity [1] and its activity is inhibited by ADP.[3] Inositol supplementation represses ethanolamine kinase, decreasing the incorporation of ethanolamine into the CDP-ethanolamine pathway and into phosphatidylethanolamine and phosphatidylcholine.[4]
References
- ^ a b Gibellini F, Hunter WN, Smith TK (October 2008). "Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline kinases". Biochem. J. 415 (1): 135–44. doi:10.1042/BJ20080435. PMC 2552378
. PMID 18489261.
- ^ Tian Y, Jackson P, Gunter C, Wang J, Rock CO, Jackowski S (September 2006). "Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2". J. Biol. Chem. 281 (38): 28438–49. doi:10.1074/jbc.M605861200. PMID 16861741.
- ^ a b Kim KH, Voelker DR, Flocco MT, Carman GM (March 1998). "Expression, purification, and characterization of choline kinase, product of the CKI gene from Saccharomyces cerevisiae". J. Biol. Chem. 273 (12): 6844–52. doi:10.1074/jbc.273.12.6844. PMID 9506987.
- ^ Kersting MC, Choi HS, Carman GM (August 2004). "Regulation of the yeast EKI1-encoded ethanolamine kinase by inositol and choline". J. Biol. Chem. 279 (34): 35353–9. doi:10.1074/jbc.M405704200. PMID 15201274.
This article incorporates text from the public domain Pfam and InterPro IPR002573
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Choline/ethanolamine kinase Provide feedback
Choline kinase catalyses the committed step in the synthesis of phosphatidylcholine by the CDP-choline pathway [1]. This alignment covers the protein kinase portion of the protein. The divergence of this family makes it very difficult to create a model that specifically predicts choline/ethanolamine kinases only. However if [add Pfam ID here for Choline_kinase_C] is also present then it is definitely a member of this family.
Literature references
-
Kim KH, Voelker DR, Flocco MT, Carman GM; , J Biol Chem 1998;273:6844-6852.: Expression, purification, and characterization of choline kinase, product of the CKI gene from Saccharomyces cerevisiae. PUBMED:9506987 EPMC:9506987
Internal database links
SCOOP: | APH APH_6_hur DUF1679 EcKinase Fructosamin_kin Kdo Kinase-like PK_Tyr_Ser-Thr Pkinase Pkinase_fungal RIO1 |
Similarity to PfamA using HHSearch: | APH EcKinase |
This tab holds annotation information from the InterPro database.
No InterPro data for this Pfam family.
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
Loading domain graphics...
Pfam Clan
This family is a member of clan PKinase (CL0016), which has the following description:
This superfamily includes the Serine/Threonine- and Tyrosine- protein kinases as well as related kinases that act on non-protein substrates.
The clan contains the following 38 members:
ABC1 AceK Act-Frag_cataly Alpha_kinase APH APH_6_hur Choline_kinase CotH DUF1679 DUF2252 DUF4135 EcKinase Fam20C Fructosamin_kin FTA2 Haspin_kinase HipA_C Ins_P5_2-kin IPK IucA_IucC Kdo Kinase-like Kinase-PolyVal KIND Pan3_PK PI3_PI4_kinase PIP49_C PIP5K PK_Tyr_Ser-Thr Pkinase Pkinase_fungal Pox_ser-thr_kin RIO1 Seadorna_VP7 UL97 WaaY YrbL-PhoP_reg YukCAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
View options
We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (31) |
Full (4855) |
Representative proteomes | UniProt (11371) |
NCBI (47421) |
Meta (1530) |
||||
---|---|---|---|---|---|---|---|---|---|
RP15 (785) |
RP35 (2210) |
RP55 (3740) |
RP75 (5555) |
||||||
Jalview | |||||||||
HTML | |||||||||
PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
— not available.
Format an alignment
Download options
We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (31) |
Full (4855) |
Representative proteomes | UniProt (11371) |
NCBI (47421) |
Meta (1530) |
||||
---|---|---|---|---|---|---|---|---|---|
RP15 (785) |
RP35 (2210) |
RP55 (3740) |
RP75 (5555) |
||||||
Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_1165 (release 4.1) |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Bateman A |
Number in seed: | 31 |
Number in full: | 4855 |
Average length of the domain: | 197.60 aa |
Average identity of full alignment: | 23 % |
Average coverage of the sequence by the domain: | 48.81 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
|
||||||||||||
Model details: |
|
||||||||||||
Model length: | 213 | ||||||||||||
Family (HMM) version: | 21 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
Sunburst controls
HideWeight segments by...
Change the size of the sunburst
Colour assignments
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
Selections
Align selected sequences to HMM
Generate a FASTA-format file
Clear selection
This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
Tree controls
HideThe tree shows the occurrence of this domain across different species. More...
Loading...
Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.
Interactions
There is 1 interaction for this family. More...
Choline_kinaseStructures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Choline_kinase domain has been found. There are 56 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
Loading structure mapping...