Summary: Reduced folate carrier
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The reduced folate carrier (a transmembrane glycoprotein) transports reduced folate into mammalian cells via the carrier mediated mechanism (as opposed to the receptor mediated mechanism) it also transports cytotoxic folate analogues used in chemotherapy  such as methotrexate (MTX). Mammalian cells have an absolute requirement for exogenous folates which are needed for growth, and biosynthesis of macromolecules .
Wong SC, Proefke SA, Bhushan A, Matherly LH; , J Biol Chem 1995;270:17468-17475.: Isolation of human cDNAs that restore methotrexate sensitivity and reduced folate carrier activity in methotrexate transport-defective Chinese hamster ovary cells. PUBMED:7615551 EPMC:7615551
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR002666
The reduced folate carrier (a transmembrane glycoprotein) transports reduced folate into mammalian cells via the carrier mediated mechanism (as opposed to the receptor mediated mechanism) it also transports cytotoxic folate analogues used in chemotherapy [PUBMED:9161403], such as methotrexate (MTX). Mammalian cells have an absolute requirement for exogenous folates which are needed for growth, and biosynthesis of macromolecules [PUBMED:9161403].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Cellular component||membrane (GO:0016020)|
|Molecular function||folic acid binding (GO:0005542)|
|reduced folate carrier activity (GO:0008518)|
|Biological process||transport (GO:0006810)|
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The major facilitator superfamily (MFS) is one of the two largest families of membrane transporters found on Earth . It is present ubiquitously in bacteria, archaea, and eukarya and includes members that can function by solute uniport, solute/cation symport, solute/cation antiport and/or solute/solute antiport with inwardly and/or outwardly directed polarity . All permeases of the MFS possess either 12 or 14 transmembrane helices .
The clan contains the following 25 members:Acatn ATG22 BT1 CLN3 DUF1228 DUF791 Folate_carrier FPN1 FTR1 LacY_symp MFS_1 MFS_1_like MFS_2 MFS_3 MFS_Mycoplasma Nodulin-like Nuc_H_symport Nucleoside_tran OATP PTR2 PUCC Sugar_tr TLC TRI12 UNC-93
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Curation and family details
|Seed source:||Pfam-B_1123 (release 4.2)|
|Author:||Bashton M, Bateman A|
|Number in seed:||24|
|Number in full:||433|
|Average length of the domain:||306.20 aa|
|Average identity of full alignment:||34 %|
|Average coverage of the sequence by the domain:||82.78 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||13|
|Download:||download the raw HMM for this family|
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