Summary: Integrase Zinc binding domain
Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.
This is the Wikipedia entry entitled "Integrase". More...
Integrase Edit Wikipedia article
| Integrase Zinc binding domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
solution structure of the n-terminal zn binding domain of hiv-1 integrase (e form), nmr, 38 structures
|
|||||||||
| Identifiers | |||||||||
| Symbol | Integrase_Zn | ||||||||
| Pfam | PF02022 | ||||||||
| InterPro | IPR003308 | ||||||||
| SCOP | 1wjb | ||||||||
| SUPERFAMILY | 1wjb | ||||||||
|
|||||||||
| Integrase core domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
crystal structure of rsv two-domain integrase
|
|||||||||
| Identifiers | |||||||||
| Symbol | rve | ||||||||
| Pfam | PF00665 | ||||||||
| Pfam clan | CL0219 | ||||||||
| InterPro | IPR001584 | ||||||||
| SCOP | 2itg | ||||||||
| SUPERFAMILY | 2itg | ||||||||
|
|||||||||
| Integrase DNA binding domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
|
crystal structure of rsv two-domain integrase
|
|||||||||
| Identifiers | |||||||||
| Symbol | IN_DBD_C | ||||||||
| Pfam | PF00552 | ||||||||
| InterPro | IPR001037 | ||||||||
| SCOP | 1ihw | ||||||||
| SUPERFAMILY | 1ihw | ||||||||
|
|||||||||
Retroviral integrase (IN) is an enzyme produced by a retrovirus (such as HIV) that enables its genetic material to be integrated into the DNA of the infected cell. Retroviral INs are not to be confused with phage integrases, such as λ phage integrase (Int) (see site-specific recombination).
IN is a key component in the retroviral pre-integration complex (PIC). The complex of integrase bound to cognate viral DNA (vDNA) ends has been referred to as the intasome.[1]
Structure
All retroviral IN proteins contain three canonical domains, connected by flexible linkers:
- an N-terminal HH-CC zinc-binding domain (a three-helical bundle stabilised by coordination of a Zn(II) cation)
- a catalytic core domain (RNaseH fold)
- a C-terminal DNA-binding domain (SH3 fold)[2]
Crystal and NMR structures of the individual domains and 2-domain constructs of integrases from HIV-1, HIV-2, SIV, and Rous Sarcoma Virus (RSV) have been reported, with the first structures determined in 1994.
Biochemical data and structural data suggest that retroviral IN functions as a tetramer (dimer-of-dimers). All three domains are important for multimerisation and viral DNA binding. Early in 2010, scientists solved the crystal structure of IN from prototype foamy virus (PFV) assembled on viral DNA ends.[3]
In addition, several host cellular proteins have been shown to interact with IN to facilitate the integration process. Human chromatin-associated protein LEDGF, which tightly binds HIV IN and directs HIV PIC towards highly expressed genes for integration, is an example of such a host factor.
Function
Integration occurs following production of the double-stranded viral DNA by the viral RNA/DNA-dependent DNA polymerase reverse transcriptase.
The main function of IN is to insert the viral DNA into the host chromosomal DNA, a step that is essential for HIV replication. Integration is a point of no return for the cell, which becomes a permanent carrier of the viral genome (provirus). Integration is in part responsible for the persistence of retroviral infections. After integration, the viral gene expression and particle production may take place immediately or at some point in the future. The timing, it is presumed, depends on the activity of the chromosomal locus hosting the provirus.
Retroviral IN catalyzes two reactions:
- 3'-processing, in which two or three nucleotides are removed from one or both 3' ends of the viral DNA to expose the invariant CA dinucleotides at both 3'-ends of the viral DNA.
- the strand transfer reaction, in which the processed 3' ends of the viral DNA are covalently ligated to the host chromosomal DNA.
Both reactions are catalysed by the same active site and occur via transesterification, without a covalent protein-DNA intermediate, in contrast to reactions catalysed by Ser and Tyr recombinases (see site specific recombination).
HIV IN
HIV integrase is a 32 kDa protein produced from the C-terminal portion of the Pol gene product, and is an attractive target for new anti-HIV drugs.
In November 2005, data from a phase 2 study of an investigational HIV integrase inhibitor, MK-0518, demonstrated that the compound has potent antiviral activity.[4][5] On October 12, 2007, the Food and Drug Administration (U.S.) approved the integrase inhibitor Raltegravir (MK-0518, brand name Isentress).[6] The second integrase inhibitor, elvitegravir, was approved in the U.S. in August 2012.[7]
The crystal structure of human foamy virus integrase has been examined successfully.[8]
This protein may use the morpheein model of allosteric regulation.[9]
See also
References
- ^ Masuda, Takao (2011-01-01). "Non-enzymatic functions of retroviral integrase: the next target for novel anti-HIV drug development". Virology. 2: 210. PMC 3192317
. PMID 22016749. doi:10.3389/fmicb.2011.00210. - ^ Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM (August 1995). "Solution structure of the DNA binding domain of HIV-1 integrase". Biochemistry. 34 (31): 9826–33. PMID 7632683. doi:10.1021/bi00031a002.
- ^ "Scientists say crack HIV/AIDS puzzle for drugs". Reuters. January 31, 2010.
- ^ Morales-Ramirez JO, Teppler H, Kovacs C, et al. Antiretroviral effect of MK-0518, a novel HIV-1 integrase inhibitor, in ART-naïve HIV-1 infected patients. Program and abstracts of the 10th European AIDS Conference; November 17–20, 2005; Dublin, Ireland. Abstract LBPS1/6. Online summary: http://clinicaloptions.com/HIV/Conference%20Coverage/Dublin%202005/Capsules/LBPS1-6.aspx
- ^ Savarino A (December 2006). "A historical sketch of the discovery and development of HIV-1 integrase inhibitors". Expert Opin Investig Drugs. 15 (12): 1507–22. PMID 17107277. doi:10.1517/13543784.15.12.1507.
- ^ "FDA approves drug that fights HIV in new way - CNN.com". CNN. October 12, 2007. Retrieved May 5, 2010.
- ^ Sax PE, DeJesus E, Mills A, Zolopa A, Cohen C, Wohl D, Gallant JE, Liu HC, Zhong L, Yale K, White K, Kearney BP, Szwarcberg J, Quirk E, Cheng AK (June 2012). "Co-formulated elvitegravir, cobicistat, emtricitabine, and tenofovir versus co-formulated efavirenz, emtricitabine, and tenofovir for initial treatment of HIV-1 infection: a randomised, double-blind, phase 3 trial, analysis of results after 48 weeks". Lancet. 379 (9835): 2439–48. PMID 22748591. doi:10.1016/S0140-6736(12)60917-9.
- ^ Hare S, Gupta SS, Valkov E, Engelman A, Cherepanov P (March 2010). "Retroviral intasome assembly and inhibition of DNA strand transfer". Nature. 464 (7286): 232–6. PMC 2837123 . PMID 20118915. doi:10.1038/nature08784.
- ^ Selwood T, Jaffe EK (March 2012). "Dynamic dissociating homo-oligomers and the control of protein function". Arch. Biochem. Biophys. 519 (2): 131–43. PMC 3298769 . PMID 22182754. doi:10.1016/j.abb.2011.11.020.
External links
- Integrases at the US National Library of Medicine Medical Subject Headings (MeSH)
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Integrase Zinc binding domain Provide feedback
Integrase mediates integration of a DNA copy of the viral genome into the host chromosome. Integrase is composed of three domains. This domain is the amino-terminal domain zinc binding domain. The central domain is the catalytic domain PF00665. The carboxyl terminal domain is a DNA binding domain PF00552.
Literature references
-
Cai M, Zheng R, Caffrey M, Craigie R, Clore GM, Gronenborn AM; , Nat Struct Biol 1997;4:567-577.: Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. PUBMED:9228950 EPMC:9228950
Internal database links
| Similarity to PfamA using HHSearch: | zf-H2C2 |
External database links
| SCOP: | 1wjb |
This tab holds annotation information from the InterPro database.
InterPro entry IPR003308
Retroviral integrase mediates integration of a DNA copy of the viral genome into the host chromosome. Integrase is composed of three domains: an N-terminal zinc binding domain, a central catalytic core and a C-terminal DNA-binding domain [PUBMED:11743009, PUBMED:11101216]. Often found as part of the POL polyprotein.
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
| Molecular function | zinc ion binding (GO:0008270) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
Loading domain graphics...
Alignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
View options
We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
| Seed (23) |
Full (211) |
Representative proteomes | UniProt (31472) |
NCBI (28301) |
Meta (0) |
||||
|---|---|---|---|---|---|---|---|---|---|
| RP15 (18) |
RP35 (27) |
RP55 (99) |
RP75 (211) |
||||||
| Jalview | |||||||||
| HTML | |||||||||
| PP/heatmap | 1 | ||||||||
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
— not available.
Format an alignment
Download options
We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
| Seed (23) |
Full (211) |
Representative proteomes | UniProt (31472) |
NCBI (28301) |
Meta (0) |
||||
|---|---|---|---|---|---|---|---|---|---|
| RP15 (18) |
RP35 (27) |
RP55 (99) |
RP75 (211) |
||||||
| Raw Stockholm | |||||||||
| Gzipped | |||||||||
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
| Seed source: | PSI-BLAST 1wjb |
| Previous IDs: | none |
| Type: | Domain |
| Author: | Bateman A |
| Number in seed: | 23 |
| Number in full: | 211 |
| Average length of the domain: | 37.70 aa |
| Average identity of full alignment: | 44 % |
| Average coverage of the sequence by the domain: | 9.41 % |
HMM information
| HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
|
||||||||||||
| Model details: |
|
||||||||||||
| Model length: | 38 | ||||||||||||
| Family (HMM) version: | 18 | ||||||||||||
| Download: | download the raw HMM for this family |
Species distribution
Sunburst controls
HideWeight segments by...
Change the size of the sunburst
Colour assignments
Archea
|
Eukaryota
|
Bacteria
|
Other sequences
|
Viruses
|
Unclassified
|
Viroids
|
Unclassified sequence
|
Selections
Align selected sequences to HMM
Generate a FASTA-format file
Clear selection
This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
Tree controls
HideThe tree shows the occurrence of this domain across different species. More...
Loading...
Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.
Interactions
Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Integrase_Zn domain has been found. There are 71 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
Loading structure mapping...