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1  structure 139  species 0  interactions 170  sequences 2  architectures

Family: STE2 (PF02116)

Summary: Fungal pheromone mating factor STE2 GPCR

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This is the Wikipedia entry entitled "Fungal mating pheromone receptors". More...

Fungal mating pheromone receptors Edit Wikipedia article

Fungal pheromone mating factor STE2 GPCR
PDB 1pjd EBI.jpg
Structure of a Peptide Segment of the 6th Transmembrane Domain of the Saccharomyces cerevisiae alpha-Factor Receptor.[1]
Identifiers
Symbol STE2
Pfam PF02116
InterPro IPR000366
SCOP 1pjd
SUPERFAMILY 1pjd
OPM superfamily 6
OPM protein 2k9p

Fungal pheromone mating factor receptors form a distinct family of G-protein-coupled receptors.

Mating factor receptors STE2 and STE3 are integral membrane proteins that may be involved in the response to mating factors on the cell membrane.[2][3][4] The amino acid sequences of both receptors contain high proportions of hydrophobic residues grouped into 7 domains, in a manner reminiscent of the rhodopsins and other receptors believed to interact with G-proteins.

References

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Fungal pheromone mating factor STE2 GPCR Provide feedback

No Pfam abstract.

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000366

G-protein-coupled receptors, GPCRs, constitute a vast protein family that encompasses a wide range of functions (including various autocrine, paracrine and endocrine processes). They show considerable diversity at the sequence level, on the basis of which they can be separated into distinct groups. We use the term clan to describe the GPCRs, as they embrace a group of families for which there are indications of evolutionary relationship, but between which there is no statistically significant similarity in sequence [PUBMED:8170923]. The currently known clan members include the rhodopsin-like GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating pheromone receptors, and the metabotropic glutamate receptor family. There is a specialised database for GPCRs (http://www.gpcr.org/7tm/).

Little is known about the structure and function of the mating factor receptors, STE2 and STE3. It is believed, however, that they are integral membrane proteins that may be involved in the response to mating factors on the cell membrane [PUBMED:16453635, PUBMED:3001640, PUBMED:2836861]. The amino acid sequences of both receptors contain high proportions of hydrophobic residues grouped into 7 domains, in a manner reminiscent of the rhodopsins and other receptors believed to interact with G-proteins. However, while a similar 3D framework has been proposed to account for this, there is no significant sequence similarity either between STE2 and STE3, or between these and the rhodopsin-type family: the receptors thus bear their own unique '7TM' signatures.

Gene Ontology

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Domain organisation

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Alignments

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Full
(170)
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(160)
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(14)
RP35
(42)
RP55
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RP75
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  Seed
(19)
Full
(170)
Representative proteomes NCBI
(160)
Meta
(0)
RP15
(14)
RP35
(42)
RP55
(72)
RP75
(88)
Alignment:
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  Seed
(19)
Full
(170)
Representative proteomes NCBI
(160)
Meta
(0)
RP15
(14)
RP35
(42)
RP55
(72)
RP75
(88)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

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Curation View help on the curation process

Seed source: IPR000366
Previous IDs: none
Type: Family
Author: Mian N, Bateman A
Number in seed: 19
Number in full: 170
Average length of the domain: 273.90 aa
Average identity of full alignment: 32 %
Average coverage of the sequence by the domain: 65.70 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.2 26.2
Trusted cut-off 26.3 26.2
Noise cut-off 25.5 26.0
Model length: 284
Family (HMM) version: 10
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the STE2 domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.

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