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36  structures 573  species 0  interactions 8566  sequences 306  architectures

Family: Gal_Lectin (PF02140)

Summary: Galactose binding lectin domain

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This is the Wikipedia entry entitled "Galactose binding lectin domain". More...

Galactose binding lectin domain Edit Wikipedia article

Gal_Lectin
Identifiers
Symbol Gal_Lectin
Pfam PF02140
InterPro IPR000922

In molecular biology, the galactose binding lectin domain is a protein domain. It is found in many proteins including the lectin purified from sea urchin (Anthocidaris crassispina) eggs, SUEL. This lectin exists as a disulfide-linked homodimer of two subunits; the dimeric form is essential for hemagglutination activity.[1] The sea urchin egg lectin (SUEL) forms a new class of lectins. Although SUEL was first isolated as a D-galactoside binding lectin, it was later shown that it binds to L-rhamnose preferentially.[1][2] L-rhamnose and D-galactose share the same hydroxyl group orientation at C2 and C4 of the pyranose ring structure.

A cysteine-rich domain (the galactose binding lectin domain) homologous to the SUEL protein has been identified in the following proteins:[3][4][5]

References

  1. ^ a b Ozeki Y, Matsui T, Suzuki M, Titani K (March 1991). "Amino acid sequence and molecular characterization of a D-galactoside-specific lectin purified from sea urchin (Anthocidaris crassispina) eggs". Biochemistry. 30 (9): 2391–4. doi:10.1021/bi00223a014. PMID 2001368. 
  2. ^ a b Hosono M, Ishikawa K, Mineki R, Murayama K, Numata C, Ogawa Y, Takayanagi Y, Nitta K (November 1999). "Tandem repeat structure of rhamnose-binding lectin from catfish (Silurus asotus) eggs". Biochim. Biophys. Acta. 1472 (3): 668–75. doi:10.1016/S0304-4165(99)00185-3. PMID 10564781. 
  3. ^ Lelianova VG, Davletov BA, Sterling A, Rahman MA, Grishin EV, Totty NF, Ushkaryov YA (August 1997). "Alpha-latrotoxin receptor, latrophilin, is a novel member of the secretin family of G protein-coupled receptors". J. Biol. Chem. 272 (34): 21504–8. doi:10.1074/jbc.272.34.21504. PMID 9261169. 
  4. ^ Tateno H, Saneyoshi A, Ogawa T, Muramoto K, Kamiya H, Saneyoshi M (July 1998). "Isolation and characterization of rhamnose-binding lectins from eggs of steelhead trout (Oncorhynchus mykiss) homologous to low density lipoprotein receptor superfamily". J. Biol. Chem. 273 (30): 19190–7. doi:10.1074/jbc.273.30.19190. PMID 9668106. 
  5. ^ a b Krasnoperov V, Bittner MA, Holz RW, Chepurny O, Petrenko AG (February 1999). "Structural requirements for alpha-latrotoxin binding and alpha-latrotoxin-stimulated secretion. A study with calcium-independent receptor of alpha-latrotoxin (CIRL) deletion mutants". J. Biol. Chem. 274 (6): 3590–6. doi:10.1074/jbc.274.6.3590. PMID 9920906. 

This article incorporates text from the public domain Pfam and InterPro IPR000922

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Galactose binding lectin domain Provide feedback

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External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR000922

The D-galactoside binding lectin purified from sea urchin (Anthocidaris crassispina) eggs exists as a disulphide-linked homodimer of two subunits; the dimeric form is essential for hemagglutination activity [ PUBMED:2001368 ]. The sea urchin egg lectin (SUEL) forms a new class of lectins. Although SUEL was first isolated as a D-galactoside binding lectin, it was latter shown that it bind to L-rhamnose preferentially [ PUBMED:2001368 , PUBMED:10564781 ]. L-rhamnose and D-galactose share the same hydroxyl group orientation at C2 and C4 of the pyranose ring structure.

A cysteine-rich domain homologous to the SUEL protein has been identified in the following proteins [ PUBMED:9261169 , PUBMED:9668106 , PUBMED:9920906 ]:

  • Plant beta-galactosidases ( EC ) (lactases).
  • Mammalian latrophilin, the calcium independent receptor of alpha-latrotoxin (CIRL). The galactose-binding lectin domain is not required for alpha-latratoxin binding [ PUBMED:9920906 ].
  • Human lectomedin-1.
  • Rhamnose-binding lectin (SAL) from catfish (Silurus asotus, Namazu) eggs. This protein is composed of three tandem repeat domains homologous to the SUEL lectin domain. All cysteine positions of each domain are completely conserved [ PUBMED:10564781 ].
  • The hypothetical B0457.1, F32A7.3A and F32A7.3B proteins from Caenorhabditis elegans.
  • The human KIAA0821 protein.

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(75)
Full
(8566)
Representative proteomes UniProt
(14733)
RP15
(1391)
RP35
(3646)
RP55
(7350)
RP75
(10158)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(75)
Full
(8566)
Representative proteomes UniProt
(14733)
RP15
(1391)
RP35
(3646)
RP55
(7350)
RP75
(10158)
Alignment:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(75)
Full
(8566)
Representative proteomes UniProt
(14733)
RP15
(1391)
RP35
(3646)
RP55
(7350)
RP75
(10158)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: IPR000922
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Mian N , Bateman A
Number in seed: 75
Number in full: 8566
Average length of the domain: 79.60 aa
Average identity of full alignment: 36 %
Average coverage of the sequence by the domain: 10.59 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 31.4 31.4
Trusted cut-off 31.5 31.4
Noise cut-off 31.3 31.3
Model length: 79
Family (HMM) version: 20
Download: download the raw HMM for this family

Species distribution

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Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Gal_Lectin domain has been found. There are 36 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0R0GSW3 View 3D Structure Click here
A0A0R0HAY2 View 3D Structure Click here
A0A0R0IE55 View 3D Structure Click here
A0A0R0J2Q1 View 3D Structure Click here
A0A0R0JAW3 View 3D Structure Click here
A0A0R0JPA1 View 3D Structure Click here
A0A0R0KAT4 View 3D Structure Click here
A0A0R0L067 View 3D Structure Click here
A0A0R4ICH5 View 3D Structure Click here
A0A0R4ICH5 View 3D Structure Click here
A0A0R4ILJ4 View 3D Structure Click here
A0A0R4ILJ4 View 3D Structure Click here
A0A0R4J2Z7 View 3D Structure Click here
A0A1D6EU33 View 3D Structure Click here
A0A1D6FPT2 View 3D Structure Click here
A0A1D6IPT8 View 3D Structure Click here
A0A1D6JPS0 View 3D Structure Click here
A0A1D6JWI7 View 3D Structure Click here
A0A1D6MYX1 View 3D Structure Click here
A0A1D6N5T1 View 3D Structure Click here
A0A1D6N5U0 View 3D Structure Click here
A0A1D6NKE5 View 3D Structure Click here
A0A1D6PKF8 View 3D Structure Click here
A0A1I9LPY2 View 3D Structure Click here
A0A286Y8J9 View 3D Structure Click here
A0A286Y8T3 View 3D Structure Click here
A0A286Y9Q9 View 3D Structure Click here
A0A2R8PV91 View 3D Structure Click here
A0A2R8PYY8 View 3D Structure Click here
A0A2R8PZB6 View 3D Structure Click here
A0A2R8Q2D4 View 3D Structure Click here
A0A2R8Q3H9 View 3D Structure Click here
A0A2R8Q5E7 View 3D Structure Click here
A0A2R8Q5V8 View 3D Structure Click here
A0A2R8Q7X2 View 3D Structure Click here
A0A2R8QAC5 View 3D Structure Click here
A0A2R8QBM5 View 3D Structure Click here
A0A2R8QBZ0 View 3D Structure Click here
A0A2R8QG91 View 3D Structure Click here
A0A2R8QHE6 View 3D Structure Click here
A0A2R8QKW9 View 3D Structure Click here
A0A2R8QM07 View 3D Structure Click here
A0A2R8QN46 View 3D Structure Click here
A0A2R8QR42 View 3D Structure Click here
A0A2R8QV94 View 3D Structure Click here
A0A2R8QV94 View 3D Structure Click here
A0A368UHP8 View 3D Structure Click here
A0A5K1K8V6 View 3D Structure Click here
A1Z7G7 View 3D Structure Click here
A3KQ75 View 3D Structure Click here
A3KQ75 View 3D Structure Click here
A3KQ78 View 3D Structure Click here
A3KQ78 View 3D Structure Click here
A3KQ79 View 3D Structure Click here
A3KQ79 View 3D Structure Click here
A3KQV6 View 3D Structure Click here
A8HAI9 View 3D Structure Click here
B0S5U6 View 3D Structure Click here
B3H4Y0 View 3D Structure Click here
B8A2F0 View 3D Structure Click here
C0P3T5 View 3D Structure Click here
D4A895 View 3D Structure Click here
D4A895 View 3D Structure Click here
E7EZV4 View 3D Structure Click here
E7F0W8 View 3D Structure Click here
E7F1D8 View 3D Structure Click here
E7F2U9 View 3D Structure Click here
E9QCY1 View 3D Structure Click here
E9QCY1 View 3D Structure Click here
E9QHT6 View 3D Structure Click here
F1QCG6 View 3D Structure Click here
F1R0Y8 View 3D Structure Click here
F1R0Y8 View 3D Structure Click here
F1R1S7 View 3D Structure Click here
G5ECX0 View 3D Structure Click here
G5EDW2 View 3D Structure Click here
H0WEB6 View 3D Structure Click here
H0WED7 View 3D Structure Click here
H1UUM0 View 3D Structure Click here
I1JCK7 View 3D Structure Click here
I1KGC4 View 3D Structure Click here
I1KUU7 View 3D Structure Click here
I1LHX5 View 3D Structure Click here
I1LJ20 View 3D Structure Click here
I1LL69 View 3D Structure Click here
I1LTJ5 View 3D Structure Click here
I1LY82 View 3D Structure Click here
I1M4L7 View 3D Structure Click here
I1M5C9 View 3D Structure Click here
I1MCY9 View 3D Structure Click here
I1MM62 View 3D Structure Click here
I1MN91 View 3D Structure Click here
I1MS58 View 3D Structure Click here
I1MSH3 View 3D Structure Click here
K7K4B2 View 3D Structure Click here
K7LTF3 View 3D Structure Click here
K7MF98 View 3D Structure Click here
K7V4R8 View 3D Structure Click here
M0R5G5 View 3D Structure Click here
O88917 View 3D Structure Click here
O88923 View 3D Structure Click here
O94910 View 3D Structure Click here
O95490 View 3D Structure Click here
P58658 View 3D Structure Click here
P58658 View 3D Structure Click here
P58659 View 3D Structure Click here
P58659 View 3D Structure Click here
Q0INM3 View 3D Structure Click here
Q0IZZ8 View 3D Structure Click here
Q10NX8 View 3D Structure Click here
Q10RB4 View 3D Structure Click here
Q1LVW4 View 3D Structure Click here
Q5N8X6 View 3D Structure Click here
Q67VU7 View 3D Structure Click here
Q6X0N7 View 3D Structure Click here
Q6ZJJ0 View 3D Structure Click here
Q75HQ3 View 3D Structure Click here
Q7G3T8 View 3D Structure Click here
Q7TN88 View 3D Structure Click here
Q7XFK2 View 3D Structure Click here
Q7Z442 View 3D Structure Click here
Q80TR1 View 3D Structure Click here
Q80TS3 View 3D Structure Click here
Q8GX69 View 3D Structure Click here
Q8JZZ7 View 3D Structure Click here
Q8RUV9 View 3D Structure Click here
Q8W0A1 View 3D Structure Click here
Q9C6W4 View 3D Structure Click here
Q9HAR2 View 3D Structure Click here
Q9LF87 View 3D Structure Click here
Q9SCQ3 View 3D Structure Click here
Q9SCU8 View 3D Structure Click here
Q9SCU9 View 3D Structure Click here
Q9SCV1 View 3D Structure Click here
Q9SCV3 View 3D Structure Click here
Q9SCV4 View 3D Structure Click here
Q9SCV5 View 3D Structure Click here
Q9SCV9 View 3D Structure Click here
Q9SCW1 View 3D Structure Click here
Q9XU98 View 3D Structure Click here
Q9Z173 View 3D Structure Click here