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3  structures 948  species 0  interactions 2709  sequences 48  architectures

Family: KA1 (PF02149)

Summary: Kinase associated domain 1

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "KIN2/PAR-1/MARK kinase family". More...

KIN2/PAR-1/MARK kinase family Edit Wikipedia article

Kinase associated domain 1
PDB 1ul7 EBI.jpg
solution structure of kinase associated domain 1 of mouse map/microtubule affinity-regulating kinase 3
Identifiers
Symbol KA1
Pfam PF02149
InterPro IPR001772
SCOP 1ul7
SUPERFAMILY 1ul7

In molecular biology, members of the KIN2/PAR-1/MARK kinase family of proteins are kinases which are conserved from yeast to human and share the same domain organisation: an N-terminal kinase domain and a C-terminal kinase associated domain 1 (KA1). Some members of this family also contain an UBA domain (ubiquitin-associated domain). Members of this kinase family are involved in various biological processes such as cell polarity, cell cycle control, intracellular signalling, microtubule stability and protein stability.[1] The function of the KA1 domain is not yet known.

Some proteins known to contain a KA1 domain are listed below:

References

  1. ^ Tassan JP, Le Goff X (April 2004). "An overview of the KIN1/PAR-1/MARK kinase family". Biol. Cell. 96 (3): 193–9. doi:10.1016/j.biolcel.2003.10.009. PMID 15182702. 
  2. ^ Biernat J, Wu YZ, Timm T, Zheng-Fischhofer Q, Mandelkow E, Meijer L, Mandelkow EM (November 2002). "Protein kinase MARK/PAR-1 is required for neurite outgrowth and establishment of neuronal polarity". Mol. Biol. Cell. 13 (11): 4013–28. doi:10.1091/mbc.02-03-0046. PMC 133611Freely accessible. PMID 12429843. 
  3. ^ Guo S, Kemphues KJ (May 1995). "par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed". Cell. 81 (4): 611–20. doi:10.1016/0092-8674(95)90082-9. PMID 7758115. 
  4. ^ Elbert M, Rossi G, Brennwald P (February 2005). "The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery". Mol. Biol. Cell. 16 (2): 532–49. doi:10.1091/mbc.E04-07-0549. PMC 545889Freely accessible. PMID 15563607. 

This article incorporates text from the public domain Pfam and InterPro IPR001772

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Kinase associated domain 1 Provide feedback

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Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR001772

Members of the KIN2/PAR-1/MARK kinase subfamily are conserved from yeast to human and share the same domain organisation: an N-terminal kinase domain (INTERPRO) and a C-terminal kinase associated domain 1 (KA1). Some members of the KIN1/PAR-1/MARK family also contain an UBA domain (INTERPRO). Members of this kinase subfamily are involved in various biological processes such as cell polarity, cell cycle control, intracellular signalling, microtubule stability and protein stability [PUBMED:15182702]. The function of the KA1 domain is not yet known.

Some proteins known to contain a KA1 domain are listed below:

  • Mammalian MAP/microtubule affinity-regulating kinases (MARK 1,2,3). They regulate polarity in neuronal cell models and appear to function redundantly in phosphorylating MT-associated proteins and in regulating MT stability [PUBMED:12429843].
  • Mammalian maternal embryonic leucine zipper kinase (MELK). It phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may be involved in the inhibition of spliceosome assembly during mitosis.
  • Caenorhabditis elegans and drosophila PAR-1 protein. It is required for establishing polarity in embryos where it is asymmetrically distributed [PUBMED:7758115].
  • Fungal Kin1 and Kin2 protein kinases involved in regulation of exocytosis. They localise to the cytoplasmic face of the plasma membrane [PUBMED:15563607].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan KA1-like (CL0573), which has the following description:

This superfamily is characterised by small adenylate-sensing domains at the C-terminus of molecules such as AMP-Activated Protein Kinases an SNF1-like protein kinases [1].

The clan contains the following 3 members:

AdenylateSensor KA1 NAF

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(96)
Full
(2709)
Representative proteomes UniProt
(4021)
NCBI
(8461)
Meta
(1)
RP15
(627)
RP35
(1247)
RP55
(1844)
RP75
(2188)
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PP/heatmap 1 View               

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(96)
Full
(2709)
Representative proteomes UniProt
(4021)
NCBI
(8461)
Meta
(1)
RP15
(627)
RP35
(1247)
RP55
(1844)
RP75
(2188)
Alignment:
Format:
Order:
Sequence:
Gaps:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(96)
Full
(2709)
Representative proteomes UniProt
(4021)
NCBI
(8461)
Meta
(1)
RP15
(627)
RP35
(1247)
RP55
(1844)
RP75
(2188)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: IPR001772
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Mian N , Bateman A
Number in seed: 96
Number in full: 2709
Average length of the domain: 44.30 aa
Average identity of full alignment: 36 %
Average coverage of the sequence by the domain: 5.56 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.8 20.8
Trusted cut-off 20.8 20.8
Noise cut-off 20.7 20.7
Model length: 45
Family (HMM) version: 19
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the KA1 domain has been found. There are 3 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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