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1  structure 294  species 0  interactions 670  sequences 34  architectures

Family: Dishevelled (PF02377)

Summary: Dishevelled specific domain

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Dishevelled Edit Wikipedia article

Dishevelled specific domain
PBB Protein DVL1 image.jpg
Solution structure of the mouse Dvl-1 DEP domain based on the PDB: 1fsh​ coordinates.
Identifiers
Symbol Dishevelled
Pfam PF02377
InterPro IPR003351
PROSITE PDOC50841

Dishevelled (Dsh) is a family of proteins involved in canonical and non-canonical Wnt signalling pathways. Dsh (Dvl in mammals) is a cytoplasmic phosphoprotein that acts directly downstream of frizzled receptors.[1] It takes its name from its initial discovery in flies, where a mutation in the dishevelled gene was observed to cause improper orientation of body and wing hairs.[2] There are vertebrae homologs in zebrafish, Xenopus (Xdsh), mice (Dvl1, -2, -3) and humans (DVL-1, -2, -3). Dsh relays complex Wnt signals in tissues and cells, in normal and abnormal contexts.[2] [3] It is thought to interact with the novel protein, SPATS1, when regulating the Wnt Signalling pathway.[4]

Dishevelled plays important roles in both the embryo and the adult, ranging from cellular differentiation and cell polarity to social behavior.[2]

Members

There are three human genes that encode for the dishevelled proteins:[5]

Overview of signal transduction pathways involved in apoptosis.

Function

Wnt pathway

DVL is an integral part of the Wnt canonical pathway (β-catenin dependent) and non-canonical pathway (β-catenin-independent).[2] In either of these, DVL acts downstream of a Frizzled receptor, although the pathways are distinct.[6]

Canonical pathway

The Wnt canonical pathway, also known as the Wnt/β-catenin pathway, is activated during development, regulation cell differentiation and proliferation.[7] The Wnt canonical pathway moves DVL between the cytoplasm and nucleus, via a conserved nuclear export sequence (NES) and a nuclear localization sequence (NLS), both necessary for proper functioning.[3] The binding of Wnt to Frizzled receptors helps recruit DVL to the membrane, providing a site for Axin and GSK3β to bind and phosphorylate LRP5/6 (transmembrane low-density lipoprotein receptor-related protein), preventing constitutive degradation of β-catenin.[6][7] The prevention of this degradation DVL allows for β-catenin buildup in the nucleus, where it acts as a coactivator for TCF (T cell factor) to activate Wnt responsive genes.[3][7] Conversely, without Wnt signaling, the destruction complex, made of APC, CKI, GSK3β and Axin, degrades β-catenin buildup, keeping the concentration of β-catenin in the cell low.[7]

Wnt non-canonical pathways

Planar cell polarity pathway

The planar cell polarity pathway (PCP) is the most notable β-catenin pathway – the Wnt signal is received by the Frizzled receptor, which relays signals to DVL, which then acts as a branch point for two independent pathways, leading to the activation of small GTPases Rho and Rac.[3][7] For the Rho branch, Wnt signals induce DVL to form a complex with Daam1 (Dishevelled associated activator of morphogenesis 1).[3] This complex then interacts with Rho guanine nucleotide exchange factor WGEF (weak-similarity GEF), which activates downstream effectors like Rho GTPase and Rho-associated kinase (ROCK), which activates actin and cytoskeleton architecture in the cell. For the Rac branch, DVL activates the Rac GTPase.[3] Activating the Rac GTPase stimulates the downstream effector c-Jun N-terminal kinase (JNK), which controls rearrangements in the cytoskeleton and gene expression.[7] More specifically, it regulates the polarity and movement of a cell, in processes in vertebrates (like Xenopus) including gastrulation, neural tube closure, and stereocilia orientation in the inner ear.[7]

Wnt-calcium pathway

Another notable β-catenin pathway is the Wnt-Ca2+ pathway, which is involved in cancer, inflammation, and neurodegeneration. Wnt triggers Frizzled-mediated activation, triggering a cascade leading to Ca2+ release, which activates effectors that control gene transcription relevant to cell fate and cell migration.[7]

Structure

There are five main highly conserved regions that exist in all variations of DVL. These include an amino-terminal DIX (N-terminus) domain, a PDZ (central) domain, a carboxyl-terminal DEP (C-terminus) domain, and two regions with positively charged amino acid residues.[3] There is a proline-heavy region between the DIX and PDZ domains, and a largely basic region between the DIX and PDZ domains that has conserved serine and threonine residues. These regions mediate protein-protein interactions and help DVL channel signals into either the β-catenin or the β-catenin independent pathways.[3] Additionally, there is the conserved nuclear export sequence (NES) and a nuclear localization sequence (NLS), whose ability to move DVL between the cytoplasm and the nucleus may be an important part of it's function.[3]

General structure of basic Dvl protein.

DIX Domain (Dishevelled-Axin)

Located near the N-terminus region of DVL and consisting of about 82-85 amino acids for human DVL protein, DIX is found in proteins like Axin and coiled-coil protein DIX-domain-containing I (DIXdc1 or Ccd1). The DIX domain of DVL has five β-strands and one α helix with highly conserved amino acid residues.[3][6]

PDZ Domain

PDZ, whose name consists of the initials of first three identified proteins to share this common structural domain (Post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (Dlg1), and Zonula occludens-1 protein (zo-1)), lies in the central region of DVL. PDZ typically has about 73 amino acids in each human DVL protein, and consists of 5-6 β-strands and 2-3 α-helices [3][6] This motif plays a critical role in ligand binding and conformational properties of the DVL protein. This region mediates many protein-protein interactions and regulates multiple biological processes.[3]

DEP Domain (Dishevelled-EGL-10-Pleackstrin)

DEP, which is in C-terminal domain of DVL, has 75 amino acids in the human DVL proteins, and has three α-helices, a β-hairpin arm, and two short β-strands.[3][6] This domain enables interaction between DVL and DAAM1, thus activating the non-canonical pathway. This domain also has results that support claims that the DEP domain is what is responsible for targeting DVL proteins to the membrane upon Wnt signal stimulation. The DEP domain may also be essential for the assembly of functional signalosomes and for Wnt signal transduction to the nucleus.[3]

NES and NLS regions

In addition to these conserved regions, DVL has both a NES and NLS, which regulate the cellular localization of DVL via movement between the nucleus and the cytoplasm. The NLS is between the PDZ and DEP domains, and the NES is between the DEP and C-terminus of DVL.[3]

Dishevelled Post-translational Modifications

There are three mains types of DVL post-translational modification - phosphorylation, ubiquitination, and methylation. Phosphorylation is the most well-studied, and seems to act such that site-specific phosphorylation can bring about a wide variety of biological responses.[3] Ubiquitination is the post-translational modification that has a role in regulating DVL degradation.

Alignment of dishevelled-specific domain

Dishevelled alignment.png

References

  1. ^ Penton A, Wodarz A, Nusse R (June 2002). "A mutational analysis of dishevelled in Drosophila defines novel domains in the dishevelled protein as well as novel suppressing alleles of axin". Genetics. 161 (2): 747–62. PMC 1462152Freely accessible. PMID 12072470. 
  2. ^ a b c d Wallingford JB, Habas R (October 2005). "The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity". Development. 132 (20): 4421–36. doi:10.1242/dev.02068. PMID 16192308. 
  3. ^ a b c d e f g h i j k l m n o p Sharma M, Castro-Piedras I, Simmons GE, Pruitt K (July 2018). "Dishevelled: A masterful conductor of complex Wnt signals". Cellular Signalling. 47: 52–64. doi:10.1016/j.cellsig.2018.03.004. PMID 29559363. 
  4. ^ Zhang H, Zhang H, Zhang Y, Ng SS, Ren F, Wang Y, Duan Y, Chen L, Zhai Y, Guo Q, Chang Z (November 2010). "Dishevelled-DEP domain interacting protein (DDIP) inhibits Wnt signaling by promoting TCF4 degradation and disrupting the TCF4/beta-catenin complex". Cellular Signalling. 22 (11): 1753–60. doi:10.1016/j.cellsig.2010.06.016. PMID 20603214. 
  5. ^ Lee YN, Gao Y, Wang HY (February 2008). "Differential mediation of the Wnt canonical pathway by mammalian Dishevelleds-1, -2, and -3". Cellular Signalling. 20 (2): 443–52. doi:10.1016/j.cellsig.2007.11.005. PMC 2233603Freely accessible. PMID 18093802. 
  6. ^ a b c d e Mlodzik M (2016). "The Dishevelled Protein Family: Still Rather a Mystery After Over 20 Years of Molecular Studies". Current Topics in Developmental Biology. 117: 75–91. doi:10.1016/bs.ctdb.2015.11.027. ISBN 9780128013823. PMC 4939608Freely accessible. PMID 26969973. 
  7. ^ a b c d e f g h Gómez-Orte E, Sáenz-Narciso B, Moreno S, Cabello J (September 2013). "Multiple functions of the noncanonical Wnt pathway". Trends in Genetics. 29 (9): 545–53. doi:10.1016/j.tig.2013.06.003. PMID 23846023. 

External links

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Dishevelled specific domain Provide feedback

This domain is specific to the signalling protein dishevelled. The domain is found adjacent to the PDZ domain PF00595 often in conjunction with DEP (PF00610) and DIX (PF00778). Much of it is disordered and yet conserved.

Literature references

  1. Theisen H, Purcell J, Bennett M, Kansagara D, Syed A, Marsh JL; , Development 1994;120:347-360.: dishevelled is required during wingless signaling to establish both cell polarity and cell identity. PUBMED:8149913 EPMC:8149913


This tab holds annotation information from the InterPro database.

InterPro entry IPR003351

This domain is specific to the signalling protein dishevelled. Dishevelled (Dsh/Dvl) is a highly conserved protein family that plays an important role in mediating Wnt signaling. Wnt signal transduction pathways control a variety of developmental and homeostatic events. Dishevelled is involved in both the canonical and non-canonical (B-catenin-independent) pathways [PUBMED:20006983].

This domain is found adjacent to the PDZ domain (INTERPRO), often in conjunction with DEP (INTERPRO) and DIX (INTERPRO).

Domain organisation

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Alignments

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  Seed
(23)
Full
(670)
Representative proteomes UniProt
(1105)
NCBI
(1995)
Meta
(0)
RP15
(131)
RP35
(264)
RP55
(480)
RP75
(559)
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  Seed
(23)
Full
(670)
Representative proteomes UniProt
(1105)
NCBI
(1995)
Meta
(0)
RP15
(131)
RP35
(264)
RP55
(480)
RP75
(559)
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  Seed
(23)
Full
(670)
Representative proteomes UniProt
(1105)
NCBI
(1995)
Meta
(0)
RP15
(131)
RP35
(264)
RP55
(480)
RP75
(559)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1381 (release 5.2)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Mian N , Bateman A
Number in seed: 23
Number in full: 670
Average length of the domain: 141.90 aa
Average identity of full alignment: 42 %
Average coverage of the sequence by the domain: 22.81 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.9 26.9
Trusted cut-off 26.9 26.9
Noise cut-off 26.7 26.7
Model length: 162
Family (HMM) version: 15
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Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Dishevelled domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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