Summary: Maf-like protein
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Maf-like protein Provide feedback
Maf is a putative inhibitor of septum formation [1] in eukaryotes, bacteria, and archaea.
Literature references
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Butler YX, Abhayawardhane Y, Stewart GC; , J Bacteriol 1993;175:3139-3145.: Amplification of the Bacillus subtilis maf gene results in arrested septum formation. PUBMED:8387996 EPMC:8387996
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Levin PA, Margolis PS, Setlow P, Losick R, Sun D; , J Bacteriol 1992;174:6717-6728.: Identification of Bacillus subtilis genes for septum placement and shape determination. PUBMED:1400224 EPMC:1400224
External database links
SCOP: | 1exc |
This tab holds annotation information from the InterPro database.
InterPro entry IPR003697
The Maf protein of Bacillus subtilis shares substantial amino acid sequence identity with Escherichia coli YhdE (previously known as OrfE) [ PUBMED:8387996 ]. Maf-like proteins are conserved in bacteria, archaea, and eukaryotes. Maf proteins have been implicated in cell division arrest [ PUBMED:21564336 ]. It has also been proposed that they belong to a family of house-cleaning nucleotide hydrolyzing enzymes which prevent the incorporation of noncanonical nucleotides into cellular DNA [ PUBMED:16359314 ]. Maf proteins exhibit nucleotide pyrophosphatase activity against canonical and modified nucleotides, which might represent a molecular mechanism for this dual role in cell division arrest and in house-cleaning [ PUBMED:24210219 ].
This entry includes pyrophosphatases, such as YhdE and YceF from E. coli. YhdE is a nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP [ PUBMED:24210219 , PUBMED:25658941 , PUBMED:28811554 ]. YceF is a nucleoside triphosphate pyrophosphatase that hydrolyzes 7-methyl-GTP (m7GTP) [ PUBMED:4210219 ].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Molecular function | nucleoside-triphosphate diphosphatase activity (GO:0047429) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan Maf (CL0269), which has the following description:
This superfamily includes the Maf-like proteins and ITPases related to YjjX [1].
The clan contains the following 4 members:
Ham1p_like HcgB Maf NTPase_I-TAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (1102) |
Full (11700) |
Representative proteomes | UniProt (51505) |
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RP15 (1710) |
RP35 (5665) |
RP55 (11519) |
RP75 (19343) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (1102) |
Full (11700) |
Representative proteomes | UniProt (51505) |
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RP15 (1710) |
RP35 (5665) |
RP55 (11519) |
RP75 (19343) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
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Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
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Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | COGs |
Previous IDs: | none |
Type: | Domain |
Sequence Ontology: | SO:0000417 |
Author: |
Mian N |
Number in seed: | 1102 |
Number in full: | 11700 |
Average length of the domain: | 185.60 aa |
Average identity of full alignment: | 31 % |
Average coverage of the sequence by the domain: | 84.19 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 189 | ||||||||||||
Family (HMM) version: | 16 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Maf domain has been found. There are 14 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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