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2  structures 1217  species 0  interactions 1540  sequences 8  architectures

# Summary: Inorganic H+ pyrophosphatase

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Inorganic diphosphatase". More...

# Inorganic diphosphatase

inorganic diphosphatase
Identifiers
EC number 3.6.1.1
CAS number 9024-82-2
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

In enzymology, an inorganic diphosphatase (EC 3.6.1.1) is an enzyme that catalyzes the chemical reaction

diphosphate + H2O $\rightleftharpoons$ 2 phosphate

Thus, the two substrates of this enzyme are diphosphate and H2O, whereas its product is phosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing anhydrides. The systematic name of this enzyme class is diphosphate phosphohydrolase. This enzyme participates in oxidative phosphorylation. It employs one cofactor, metal.

## References

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

# Inorganic H+ pyrophosphatase

The H+ pyrophosphatase is an transmembrane proton pump involved in establishing the H+ electrochemical potential difference between the vacuole lumen and the cell cytosol. Vacuolar-type H(+)-translocating inorganic pyrophosphatases have long been considered to be restricted to plants and to a few species of photo-trophic bacteria. However, in recent investigations, these pyrophosphatases have been found in organisms as disparate as thermophilic Archaea and parasitic protists [1].

## Literature references

1. Drozdowicz YM, Rea PA; , Trends Plant Sci 2001;6:206-211.: Vacuolar H(+) pyrophosphatases: from the evolutionary backwaters into the mainstream. PUBMED:11335173 EPMC:11335173

This tab holds annotation information from the InterPro database.

# InterPro entry IPR004131

Two types of proteins that hydrolyse inorganic pyrophosphate (PPi), very different in both amino acid sequence and structure, have been characterised to date: soluble and membrane-bound proton-pumping pyrophosphatases (sPPases and H(+)-PPases, respectively). sPPases are ubiquitous proteins that hydrolyse PPi to release heat, whereas H+-PPases, so far unidentified in animal and fungal cells, couple the energy of PPi hydrolysis to proton movement across biological membranes [PUBMED:12451180, PUBMED:10523139]. The latter type is represented by this group of proteins. H+-PPases (EC) are also called vacuolar-type inorganic pyrophosphatases (V-PPase) or pyrophosphate-energised vacuolar membrane proton pumps [PUBMED:11343697]. In plants, vacuoles contain two enzymes for acidifying the interior of the vacuole, the V-ATPase and the V-PPase (V is for vacuolar) [PUBMED:10523139].

Two distinct biochemical subclasses of H+-PPases have been characterised to date: K+-stimulated and K+-insensitive [PUBMED:12451180, PUBMED:11343697].

### Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

# Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

# Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...

## View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Seed
(139)
Full
(1540)
Representative proteomes NCBI
(1460)
Meta
(5022)
RP15
(268)
RP35
(465)
RP55
(553)
RP75
(611)
Jalview View  View  View  View  View  View  View  View
HTML View  View  View  View  View  View
PP/heatmap 1 View  View  View  View  View
Pfam viewer View  View

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: available, not generated, not available.

## Format an alignment

Seed
(139)
Full
(1540)
Representative proteomes NCBI
(1460)
Meta
(5022)
RP15
(268)
RP35
(465)
RP55
(553)
RP75
(611)
Alignment:
Format:
Order:
Sequence:
Gaps:

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Seed
(139)
Full
(1540)
Representative proteomes NCBI
(1460)
Meta
(5022)
RP15
(268)
RP35
(465)
RP55
(553)
RP75
(611)

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.

# HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

# Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

# Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

## Curation

 Seed source: Pfam-B_1050 (release 6.4) Previous IDs: none Type: Family Author: Griffiths-Jones SR Number in seed: 139 Number in full: 1540 Average length of the domain: 626.60 aa Average identity of full alignment: 43 % Average coverage of the sequence by the domain: 93.38 %

## HMM information

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.2 22.2
Trusted cut-off 22.4 22.4
Noise cut-off 22.1 22.1
Model length: 682
Family (HMM) version: 11

# Species distribution

### Sunburst controls

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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