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The lef-2 gene (for late expression factor 2) from baculovirus is required for expression of late genes. This gene has been shown to be specifically required for expression from the vp39 and polh promoters . LEF-1 is a DNA primase and there is some evidence to suggest that LEF-2 may bind to both DNA and LEF-1 .
Ahrens CH, Rohrmann GF; , Virology 1995;212:650-662.: Replication of Orgyia pseudotsugata baculovirus DNA: lef-2 and ie-1 are essential and ie-2, p34, and Op-iap are stimulatory genes. PUBMED:7571435 EPMC:7571435
External database links
This tab holds annotation information from the InterPro database.
InterPro entry IPR004283
The late expression factor 2 (lef-2) protein from Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV) is required for expression of late genes. The lef-2 protein has been shown to be specifically required for expression from the vp39 and polh promoters [PUBMED:8445724].
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
|Biological process||viral transcription (GO:0019083)|
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This clan contains the large subunit of archaeal and eukaryotic DNA primase, an enzyme which synthesises the oligoribonucleotide primers essential to DNA replication. The large subunit of DNA primase forms interactions with the small subunit and the structure implicates that it is not directly involved in catalysis, but plays a roles in correctly positioning the primase/DNA complex, and in the transfer of RNA to DNA polymerase . The clan also contains the Lef-2 family, which is required for the expression of late genes. There is some evidence to suggest that LEF2 binds to both DNA and the DNA primase small subunit LEF-1 .
The clan contains the following 2 members:Baculo_LEF-2 DNA_primase_lrg
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Curation and family details
|Seed source:||Pfam-B_1773 (release 6.4)|
|Number in seed:||11|
|Number in full:||69|
|Average length of the domain:||164.60 aa|
|Average identity of full alignment:||35 %|
|Average coverage of the sequence by the domain:||79.78 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||9|
|Download:||download the raw HMM for this family|
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