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13  structures 729  species 0  interactions 3453  sequences 74  architectures

Family: Glyco_hydro_72 (PF03198)

Summary: Glucanosyltransferase

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Glycoside hydrolase family 72". More...

Glycoside hydrolase family 72 Edit Wikipedia article

Glucanosyltransferase
Identifiers
SymbolGlyco_hydro_72
PfamPF03198
Pfam clanCL0058
InterProIPR004886
CAZyGH72

In molecular biology, glycoside hydrolase family 72 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes. [5]

This family includes yeast glycolipid proteins anchored to the membrane. It includes Candida albicans pH-regulated protein, which is required for apical growth and plays a role in morphogenesis, and Saccharomyces cerevisiae glycolipid anchored surface protein.

References

  1. ^ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  2. ^ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
  3. ^ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
  4. ^ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
  5. ^ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
This article incorporates text from the public domain Pfam and InterPro: IPR004886

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Glucanosyltransferase Provide feedback

This is a family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases. The active site residues in the Aspergillus fumigatus example B0XT72 are the two glutamate residues at 160 and 261 [3].

Literature references

  1. Nuoffer C, Jeno P, Conzelmann A, Riezman H; , Mol Cell Biol 1991;11:27-37.: Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. PUBMED:1824714 EPMC:1824714

  2. Saporito-Irwin SM, Birse CE, Sypherd PS, Fonzi WA; , Mol Cell Biol 1995;15:601-613.: PHR1, a pH-regulated gene of Candida albicans, is required for morphogenesis. PUBMED:7823929 EPMC:7823929

  3. Mouyna I, Monod M, Fontaine T, Henrissat B, Lechenne B, Latge JP;, Biochem J. 2000;347:741-747.: Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. PUBMED:10769178 EPMC:10769178


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR004886

This is a family of yeast glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases [ PUBMED:10769178 ]. It includes Candida albicans pH-regulated protein PHR1, which is required for apical growth and plays a role in morphogenesis [ PUBMED:7823929 ] and Saccharomyces cerevisiae glycolipid anchored surface protein Gas1-5 [ PUBMED:1824714 ].

Gas1 is a beta-1,3-glucanosyltransferase that was initially characterised at the cell wall where it remodels chains of beta-1,3-glucan. Later, it was found to be involved in transcriptional silencing [ PUBMED:19541632 ] and DNA damage response [ PUBMED:24532730 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(11)
Full
(3453)
Representative proteomes UniProt
(5713)
RP15
(381)
RP35
(1308)
RP55
(2264)
RP75
(3453)
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PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(11)
Full
(3453)
Representative proteomes UniProt
(5713)
RP15
(381)
RP35
(1308)
RP55
(2264)
RP75
(3453)
Alignment:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(11)
Full
(3453)
Representative proteomes UniProt
(5713)
RP15
(381)
RP35
(1308)
RP55
(2264)
RP75
(3453)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_2209 (release 6.5)
Previous IDs: GAS1;
Type: Domain
Sequence Ontology: SO:0000417
Author: Mifsud W
Number in seed: 11
Number in full: 3453
Average length of the domain: 298.1 aa
Average identity of full alignment: 38 %
Average coverage of the sequence by the domain: 60.68 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.9 25.9
Trusted cut-off 25.9 25.9
Noise cut-off 25.8 25.8
Model length: 315
Family (HMM) version: 17
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_hydro_72 domain has been found. There are 13 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0D2GPQ7 View 3D Structure Click here
A0A0D2GQK8 View 3D Structure Click here
A0A0D2GU19 View 3D Structure Click here
A0A0D2GZT0 View 3D Structure Click here
A0A175VQ30 View 3D Structure Click here
A0A175VRP5 View 3D Structure Click here
A0A175W0Z5 View 3D Structure Click here
A0A175W1J3 View 3D Structure Click here
A0A175W8B6 View 3D Structure Click here
A0A1C1C898 View 3D Structure Click here
A0A1C1CEZ9 View 3D Structure Click here
A0A1C1CFX5 View 3D Structure Click here
A0A1C1CHE8 View 3D Structure Click here
A0A1D8PKY4 View 3D Structure Click here
C0NH39 View 3D Structure Click here
C0NKV6 View 3D Structure Click here
C0NLL2 View 3D Structure Click here
C0NSW7 View 3D Structure Click here
C1GUA5 View 3D Structure Click here
C1GZ38 View 3D Structure Click here
C1H2S5 View 3D Structure Click here
C1H3A2 View 3D Structure Click here
D4ATC3 View 3D Structure Click here
O13318 View 3D Structure Click here
O13692 View 3D Structure Click here
P0C7S9 View 3D Structure Click here
P0C954 View 3D Structure Click here
P0C955 View 3D Structure Click here
P0C956 View 3D Structure Click here
P22146 View 3D Structure Click here
P43076 View 3D Structure Click here
Q03655 View 3D Structure Click here
Q06135 View 3D Structure Click here
Q08193 View 3D Structure Click here
Q08271 View 3D Structure Click here
Q59VW6 View 3D Structure Click here
Q5AJY5 View 3D Structure Click here
Q9P378 View 3D Structure Click here
Q9USU5 View 3D Structure Click here
Q9Y7Y7 View 3D Structure Click here