Summary: Roadblock/LC7 domain
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This is the Wikipedia entry entitled "Dynein". More...
Dynein Edit Wikipedia article
Dyenin can be divided into two groups:
Cytoplasmic dynein and Axonemal dynein.
The Axonomal dynein acts to activate a sliding within flagellar microtubules, wherease the Cytoplasmic dynein is implcated in moving toward the negative end of a microtubule.
See also:
Kinesin How Cilia and Flagella Work (Axonomal Dynein) Microtubule Based Movement (Cytoplasmic dynein)
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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Roadblock/LC7 domain Provide feedback
This family includes proteins that are about 100 amino acids long and have been shown to be related [3]. Members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. It is proposed that roadblock/LC7 family members may modulate specific dynein functions [2]. This family also includes Q9Y2Q5 Golgi-associated MP1 adapter protein and MglB from Myxococcus xanthus Q50883 a protein involved in gliding motility [4]. However the family also includes members from non-motile bacteria such as Streptomyces coelicolor, suggesting that the protein may play a structural or regulatory role.
Literature references
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Hodgson JW, Argiropoulos B, Brock HW; , Mol Cell Biol 2001;21:4528-4543.: Site-specific recognition of a 70-base-pair element containing d(GA)(n) repeats mediates bithoraxoid polycomb group response element-dependent silencing. PUBMED:11416132 EPMC:11416132
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Bowman AB, Patel-King RS, Benashski SE, McCaffery JM, Goldstein LS, King SM; , J Cell Biol 1999;146:165-180.: Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. PUBMED:10402468 EPMC:10402468
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Koonin EV, Aravind L; , Curr Biol 2000;10:774-776.: Dynein light chains of the Roadblock/LC7 group belong to an ancient protein superfamily implicated in NTPase regulation. PUBMED:11084347 EPMC:11084347
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Stephens K, Hartzell P, Kaiser D; , J Bacteriol 1989;171:819-830.: Gliding motility in Myxococcus xanthus: mgl locus, RNA, and predicted protein products. PUBMED:2464581 EPMC:2464581
Internal database links
SCOOP: | LAMTOR5 MAPKK1_Int |
Similarity to PfamA using HHSearch: | LAMTOR5 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR004942
This domain can be found in the roadblock proteins and LAMTOR2 proteins.
Dynein light chain roadblock proteins (DYNLRB1 and DYNLRB2) are non-catalytic accessory components of the cytoplasmic dynein 1 complex. The dynein light chains are required for the correct assembly of the dynein complex and have been implicated in controlling its association with cargo molecules. DYNLRB1 and DYNLRB2 can also be found in the dynein-2 complex [ PUBMED:25205765 ].
LAMTOR2 is part of the ragulator complex involved in amino acid sensing and activation of mTORC1, a signalling complex promoting cell growth in response to growth factors, energy levels, and amino acids [ PUBMED:20381137 , PUBMED:22980980 ].
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan PF (CL0431), which has the following description:
The families here all show the Profilin-like fold, and represent both the Profilin (actin-binding protein) (55770) and the Roadblock/LC7 domain-type (103196) superfamilies.
The clan contains the following 21 members:
Clat_adaptor_s FNIP_N Fuz_longin_1 Fuz_longin_2 Fuz_longin_3 Intu_longin_1 Intu_longin_2 Intu_longin_3 LAMTOR5 Longin Longin_2 MAPKK1_Int Nyv1_longin Profilin Robl_LC7 Sedlin_N SLM4 SRP-alpha_N SRX Sybindin uDENNAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (37) |
Full (9334) |
Representative proteomes | UniProt (30272) |
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RP15 (941) |
RP35 (3462) |
RP55 (9477) |
RP75 (16046) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
— not available.
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (37) |
Full (9334) |
Representative proteomes | UniProt (30272) |
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RP15 (941) |
RP35 (3462) |
RP55 (9477) |
RP75 (16046) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Aravind L |
Previous IDs: | none |
Type: | Domain |
Sequence Ontology: | SO:0000417 |
Author: |
Mifsud W |
Number in seed: | 37 |
Number in full: | 9334 |
Average length of the domain: | 89.6 aa |
Average identity of full alignment: | 27 % |
Average coverage of the sequence by the domain: | 63.56 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 91 | ||||||||||||
Family (HMM) version: | 20 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Robl_LC7 domain has been found. There are 108 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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AlphaFold Structure Predictions
The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.