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108  structures 2122  species 0  interactions 9334  sequences 56  architectures

Family: Robl_LC7 (PF03259)

Summary: Roadblock/LC7 domain

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This is the Wikipedia entry entitled "Dynein". More...

Dynein Edit Wikipedia article

Dyenin can be divided into two groups:

Cytoplasmic dynein and Axonemal dynein.

The Axonomal dynein acts to activate a sliding within flagellar microtubules, wherease the Cytoplasmic dynein is implcated in moving toward the negative end of a microtubule.

See also:

Kinesin How Cilia and Flagella Work (Axonomal Dynein) Microtubule Based Movement (Cytoplasmic dynein)

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Roadblock/LC7 domain Provide feedback

This family includes proteins that are about 100 amino acids long and have been shown to be related [3]. Members of this family of proteins are associated with both flagellar outer arm dynein and Drosophila and rat brain cytoplasmic dynein. It is proposed that roadblock/LC7 family members may modulate specific dynein functions [2]. This family also includes Q9Y2Q5 Golgi-associated MP1 adapter protein and MglB from Myxococcus xanthus Q50883 a protein involved in gliding motility [4]. However the family also includes members from non-motile bacteria such as Streptomyces coelicolor, suggesting that the protein may play a structural or regulatory role.

Literature references

  1. Hodgson JW, Argiropoulos B, Brock HW; , Mol Cell Biol 2001;21:4528-4543.: Site-specific recognition of a 70-base-pair element containing d(GA)(n) repeats mediates bithoraxoid polycomb group response element-dependent silencing. PUBMED:11416132 EPMC:11416132

  2. Bowman AB, Patel-King RS, Benashski SE, McCaffery JM, Goldstein LS, King SM; , J Cell Biol 1999;146:165-180.: Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis. PUBMED:10402468 EPMC:10402468

  3. Koonin EV, Aravind L; , Curr Biol 2000;10:774-776.: Dynein light chains of the Roadblock/LC7 group belong to an ancient protein superfamily implicated in NTPase regulation. PUBMED:11084347 EPMC:11084347

  4. Stephens K, Hartzell P, Kaiser D; , J Bacteriol 1989;171:819-830.: Gliding motility in Myxococcus xanthus: mgl locus, RNA, and predicted protein products. PUBMED:2464581 EPMC:2464581

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR004942

This domain can be found in the roadblock proteins and LAMTOR2 proteins.

Dynein light chain roadblock proteins (DYNLRB1 and DYNLRB2) are non-catalytic accessory components of the cytoplasmic dynein 1 complex. The dynein light chains are required for the correct assembly of the dynein complex and have been implicated in controlling its association with cargo molecules. DYNLRB1 and DYNLRB2 can also be found in the dynein-2 complex [ PUBMED:25205765 ].

LAMTOR2 is part of the ragulator complex involved in amino acid sensing and activation of mTORC1, a signalling complex promoting cell growth in response to growth factors, energy levels, and amino acids [ PUBMED:20381137 , PUBMED:22980980 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan PF (CL0431), which has the following description:

The families here all show the Profilin-like fold, and represent both the Profilin (actin-binding protein) (55770) and the Roadblock/LC7 domain-type (103196) superfamilies.

The clan contains the following 21 members:

Clat_adaptor_s FNIP_N Fuz_longin_1 Fuz_longin_2 Fuz_longin_3 Intu_longin_1 Intu_longin_2 Intu_longin_3 LAMTOR5 Longin Longin_2 MAPKK1_Int Nyv1_longin Profilin Robl_LC7 Sedlin_N SLM4 SRP-alpha_N SRX Sybindin uDENN


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

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Representative proteomes UniProt

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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Aravind L
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Mifsud W , Bateman A
Number in seed: 37
Number in full: 9334
Average length of the domain: 89.6 aa
Average identity of full alignment: 27 %
Average coverage of the sequence by the domain: 63.56 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.0 23.0
Trusted cut-off 23.0 23.0
Noise cut-off 22.9 22.9
Model length: 91
Family (HMM) version: 20
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Robl_LC7 domain has been found. There are 108 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044QNX8 View 3D Structure Click here
A0A077Z468 View 3D Structure Click here
A0A077ZKE6 View 3D Structure Click here
A0A0J9XN11 View 3D Structure Click here
A0A0K0JRD1 View 3D Structure Click here
A0A0N4UDT7 View 3D Structure Click here
A0A2R8QEB6 View 3D Structure Click here
A0A3P7DJF5 View 3D Structure Click here
A0A3P7EGD6 View 3D Structure Click here
A0A5K4FCG2 View 3D Structure Click here
A4HXX4 View 3D Structure Click here
A4I7C2 View 3D Structure Click here
A4I8H6 View 3D Structure Click here
A4IB75 View 3D Structure Click here
B5FYY5 View 3D Structure Click here
B5X7X4 View 3D Structure Click here
C6S3C4 View 3D Structure Click here
C6S3D2 View 3D Structure Click here
D4A0A0 View 3D Structure Click here
D4A465 View 3D Structure Click here
K0EHG9 View 3D Structure Click here
K0ENN2 View 3D Structure Click here
K0EUS0 View 3D Structure Click here
K0EV28 View 3D Structure Click here
K0EYG9 View 3D Structure Click here
K0EZQ8 View 3D Structure Click here
K0F6T3 View 3D Structure Click here
O50393 View 3D Structure Click here
O67683 View 3D Structure Click here
P62627 View 3D Structure Click here
P62628 View 3D Structure Click here
Q15ET8 View 3D Structure Click here
Q22752 View 3D Structure Click here
Q32P85 View 3D Structure Click here
Q381D2 View 3D Structure Click here
Q388T6 View 3D Structure Click here
Q38D18 View 3D Structure Click here
Q3T132 View 3D Structure Click here
Q3T140 View 3D Structure Click here
Q4CT12 View 3D Structure Click here