Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
207  structures 1563  species 0  interactions 14791  sequences 227  architectures

Family: Snf7 (PF03357)

Summary: Snf7

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Snf7 Provide feedback

This family of proteins are involved in protein sorting and transport from the endosome to the vacuole/lysosome in eukaryotic cells. Vacuoles/lysosomes play an important role in the degradation of both lipids and cellular proteins. In order to perform this degradative function, vacuoles/lysosomes contain numerous hydrolases which have been transported in the form of inactive precursors via the biosynthetic pathway and are proteolytically activated upon delivery to the vacuole/lysosome. The delivery of transmembrane proteins, such as activated cell surface receptors to the lumen of the vacuole/lysosome, either for degradation/downregulation, or in the case of hydrolases, for proper localisation, requires the formation of multivesicular bodies (MVBs). These late endosomal structures are formed by invaginating and budding of the limiting membrane into the lumen of the compartment. During this process, a subset of the endosomal membrane proteins is sorted into the forming vesicles. Mature MVBs fuse with the vacuole/lysosome, thereby releasing cargo containing vesicles into its hydrolytic lumen for degradation. Endosomal proteins that are not sorted into the intralumenal MVB vesicles are either recycled back to the plasma membrane or Golgi complex, or remain in the limiting membrane of the MVB and are thereby transported to the limiting membrane of the vacuole/lysosome as a consequence of fusion. Therefore, the MVB sorting pathway plays a critical role in the decision between recycling and degradation of membrane proteins [1]. A few archaeal sequences are also present within this family.

Literature references

  1. Babst M, Katzmann DJ, Estepa-Sabal EJ, Meerloo T, Emr SD; , Dev Cell 2002;3:271-282.: Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. PUBMED:12194857 EPMC:12194857

  2. Teo H, Perisic O, Gonzalez B, Williams RL; , Dev Cell 2004;7:559-569.: ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes. PUBMED:15469844 EPMC:15469844

  3. Peck JW, Bowden ET, Burbelo PD; , Biochem J 2004;377:693-700.: Structure and function of human Vps20 and Snf7 proteins. PUBMED:14583093 EPMC:14583093

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR005024

Snf7 family members are small coil-coiled proteins that share protein sequence similarity with budding yeast Snf7, which is part of the ESCRT-III complex that is required for endosome-mediated trafficking via multivesicular body (MVB) formation and sorting [ PUBMED:15086794 ].

Proteins in this entry also includes human CHMPs (charged multivesicular body proteins), budding yeast Did4/Did2, Arabidopsis vacuolar protein sorting-associated proteins and the archaean Sulfolobus acidocaldarius cell division protein B which is a component required for cell division, forming polymers with segregating nucleoids [ PUBMED:18987308 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...

Pfam Clan

This family is a member of clan PspA (CL0235), which has the following description:

This clan includes PspA like proteins that are transcriptional activators as well as Snf7, a protein involved in cellular trafficking.

The clan contains the following 3 members:

Ist1 PspA_IM30 Snf7


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

Representative proteomes UniProt

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Representative proteomes UniProt
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_1641 (release 6.6)
Previous IDs: DUF279; SNF7; ESCRT-III;
Type: Family
Sequence Ontology: SO:0100021
Author: Mifsud W , Moxon SJ , Mistry J , Wood V
Number in seed: 32
Number in full: 14791
Average length of the domain: 168.10 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 66.94 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.3 27.3
Trusted cut-off 27.3 27.3
Noise cut-off 27.2 27.2
Model length: 173
Family (HMM) version: 24
Download: download the raw HMM for this family

Species distribution

Sunburst controls


Weight segments by...

Change the size of the sunburst


Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


Align selected sequences to HMM

Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls


The tree shows the occurrence of this domain across different species. More...


Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.


For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Snf7 domain has been found. There are 207 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0P0VL75 View 3D Structure Click here
A0A0P0XJ91 View 3D Structure Click here
A0A0R0GLI8 View 3D Structure Click here
A0A0R0J5V8 View 3D Structure Click here
A0A0R4IS77 View 3D Structure Click here
A0A144A0N0 View 3D Structure Click here
A0A144A3Q5 View 3D Structure Click here
A0A1D6FZB5 View 3D Structure Click here
A0A1D6IBE8 View 3D Structure Click here
A0A1D6JKN6 View 3D Structure Click here
A0A1D6JZ51 View 3D Structure Click here
A0A1D6KTI5 View 3D Structure Click here
A0A1D6L8F6 View 3D Structure Click here
A0A1D6MR75 View 3D Structure Click here
A0A1D6NMB1 View 3D Structure Click here
A0A1D6P3C0 View 3D Structure Click here
A0A1D6PN30 View 3D Structure Click here
A0A1D6PV38 View 3D Structure Click here
A0A1D6QGS9 View 3D Structure Click here
A0A1D8PG90 View 3D Structure Click here
A0A1D8PM89 View 3D Structure Click here
A0A1D8PNJ4 View 3D Structure Click here
A0A1X7YGK1 View 3D Structure Click here
A0A2R8PYZ7 View 3D Structure Click here
A0A381ML17 View 3D Structure Click here
A4HWY8 View 3D Structure Click here
A4I952 View 3D Structure Click here
A4IAV5 View 3D Structure Click here
A4IBY0 View 3D Structure Click here
A4ID11 View 3D Structure Click here
A4ID53 View 3D Structure Click here
B2RZB5 View 3D Structure Click here
B4FH14 View 3D Structure Click here
B4FJ73 View 3D Structure Click here
B4FU00 View 3D Structure Click here
B6T4F7 View 3D Structure Click here
B6TYU1 View 3D Structure Click here
B6U8I2 View 3D Structure Click here
C0PNJ6 View 3D Structure Click here
C6TD23 View 3D Structure Click here