Summary: Glycosyltransferase family 6
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This is the Wikipedia entry entitled "Glycoprotein-fucosylgalactoside a-N-acetylgalactosaminyltransferase". More...
Glycoprotein-fucosylgalactoside a-N-acetylgalactosaminyltransferase Edit Wikipedia article
In enzymology, a glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC 2.4.1.40) is an enzyme that catalyzes the chemical reaction
- UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl- (1,2)]-D-galactose
Thus, the two substrates of this enzyme are UDP-N-acetyl-D-galactosamine and glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose, whereas its 3 products are UDP, [[glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-]], and [[(1,2)]-D-galactose]].
This enzyme belongs to the family of transferases, specifically those glycosyltransferases hexosyltransferases. The systematic name of this enzyme class is UDP-N-acetyl-D-galactosamine:glycoprotein-alpha-L-fucosyl-(1,2)-D-ga lactose 3-N-acetyl-D-galactosaminyltransferase. Other names in common use include A-transferase, histo-blood group A glycosyltransferase, (Fucalpha1→2Galalpha1→3-N-acetylgalactosaminyltransferase), UDP-GalNAc:Fucalpha1→2Galalpha1→3-N-acetylgalactosaminyltransferase, alpha-3-N-acetylgalactosaminyltransferase, blood-group substance alpha-acetyltransferase, blood-group substance A-dependent acetylgalactosaminyltransferase, fucosylgalactose acetylgalactosaminyltransferase, histo-blood group A acetylgalactosaminyltransferase, histo-blood group A transferase, UDP-N-acetyl-D-galactosamine:alpha-L-fucosyl-1,2-D-galactose, and 3-N-acetyl-D-galactosaminyltransferase. This enzyme participates in 3 metabolic pathways: the lactoseries and neolactoseries of glycosphingolipid biosynthesis, as wellas the biosynthesis of glycan structures.
Structural studies
As of late 2007, 19 structures have been solved for this class of enzymes, with PDB accession codes 1LZ0, 1LZI, 1R7T, 1R7V, 1R7Y, 1R81, 1WSZ, 1WT0, 1WT1, 1WT2, 1WT3, 1XZ6, 1ZHJ, 1ZI1, 1ZI3, 1ZI4, 1ZI5, 1ZJO, and 2A8W.
References
- Kobata A, Grollman EF, Ginsburg V (1968). "An enzymic basis for blood type A in humans". Arch. Biochem. Biophys. 124: 609–12. PMID 5661629.
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: CS1 maint: multiple names: authors list (link) - Takeya A, Hosomi O, Ishiura M (Tokyo). "Complete purification and characterization of alpha-3-N-acetylgalactosaminyltransferase encoded by the human blood group A gene". J. Biochem.: 360–8. PMID 2341371.
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: Check date values in:|date=
(help)CS1 maint: multiple names: authors list (link) - Yates AD, Feeney J, Donald AS, Watkins WM (1984). "Characterisation of a blood-group A-active tetrasaccharide synthesised by a blood-group B gene-specified glycosyltransferase". Carbohydr. Res. 130: 251–60. PMID 6434182.
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: CS1 maint: multiple names: authors list (link)
External links
- The CAS registry number for this enzyme class is Template:CAS registry.
Gene Ontology (GO) codes
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Glycosyltransferase family 6 Provide feedback
No Pfam abstract.
External database links
CAZY: | GT6 |
SCOP: | 1fg5 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR005076
The biosynthesis of disaccharides, oligosaccharides and polysaccharides involves the action of hundreds of different glycosyltransferases. These enzymes catalyse the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. A classification of glycosyltransferases using nucleotide diphospho-sugar, nucleotide monophospho-sugar and sugar phosphates ([intenz:2.4.1.-]) and related proteins into distinct sequence based families has been described [ PUBMED:9334165 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. The same three-dimensional fold is expected to occur within each of the families. Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence similarities may have similar 3-D structures and therefore form 'clans'.
Glycosyltransferase family 6 CAZY comprises enzymes with three known activities; alpha-1,3-galactosyltransferase ( EC ); alpha-1,3 N-acetylgalactosaminyltransferase ( EC ); alpha-galactosyltransferase ( EC ).
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Cellular component | membrane (GO:0016020) |
Molecular function | hexosyltransferase activity (GO:0016758) |
Biological process | carbohydrate metabolic process (GO:0005975) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan GT-A (CL0110), which has the following description:
This is the GT-A clan that contains diverse glycosyltransferases that possess a Rossmann like fold [1].
The clan contains the following 53 members:
Anp1 Branch Caps_synth Cellulose_synt CgtA CHGN Chitin_synth_1 Chitin_synth_2 CofC CTP_transf_3 DUF2064 DUF273 DUF604 DUF6564 Fringe Galactosyl_T GlcNAc Gly_transf_sug Glyco_tranf_2_2 Glyco_tranf_2_3 Glyco_tranf_2_4 Glyco_tranf_2_5 Glyco_trans_2_3 Glyco_transf_15 Glyco_transf_21 Glyco_transf_24 Glyco_transf_25 Glyco_transf_34 Glyco_transf_43 Glyco_transf_49 Glyco_transf_6 Glyco_transf_64 Glyco_transf_7C Glyco_transf_7N Glyco_transf_8 Glyco_transf_88 Glyco_transf_92 Glycos_transf_2 GNT-I IspD Mannosyl_trans3 MGAT2 NTP_transf_3 NTP_transferase Nucleotid_trans Osmo_MPGsynth Pox_P35 RGP Rhamno_transf STELLO TcdA_TcdB UDP-g_GGTase UDPGPAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (6) |
Full (1872) |
Representative proteomes | UniProt (3358) |
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RP15 (99) |
RP35 (461) |
RP55 (1306) |
RP75 (1935) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
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not generated,
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Seed (6) |
Full (1872) |
Representative proteomes | UniProt (3358) |
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RP15 (99) |
RP35 (461) |
RP55 (1306) |
RP75 (1935) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_4383 (release 6.6) |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Bateman A |
Number in seed: | 6 |
Number in full: | 1872 |
Average length of the domain: | 272 aa |
Average identity of full alignment: | 41 % |
Average coverage of the sequence by the domain: | 84.3 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 313 | ||||||||||||
Family (HMM) version: | 16 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_transf_6 domain has been found. There are 245 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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AlphaFold Structure Predictions
The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.