Powering down the Pfam website
On October 5th, we will start redirecting the traffic from Pfam (pfam.xfam.org) to InterPro (www.ebi.ac.uk/interpro). The Pfam website will be available at pfam-legacy.xfam.org until January 2023, when it will be decommissioned. You can read more about the sunset period in our blog post.

Please note: this site relies heavily on the use of javascript. Without a javascript-enabled browser, this site will not function correctly. Please enable javascript and reload the page, or switch to a different browser.
245  structures 285  species 0  interactions 1872  sequences 28  architectures

Family: Glyco_transf_6 (PF03414)

Summary: Glycosyltransferase family 6

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "Glycoprotein-fucosylgalactoside a-N-acetylgalactosaminyltransferase". More...

Glycoprotein-fucosylgalactoside a-N-acetylgalactosaminyltransferase Edit Wikipedia article

In enzymology, a glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC is an enzyme that catalyzes the chemical reaction

UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl- (1,2)]-D-galactose

Thus, the two substrates of this enzyme are UDP-N-acetyl-D-galactosamine and glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose, whereas its 3 products are UDP, [[glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-]], and [[(1,2)]-D-galactose]].

This enzyme belongs to the family of transferases, specifically those glycosyltransferases hexosyltransferases. The systematic name of this enzyme class is UDP-N-acetyl-D-galactosamine:glycoprotein-alpha-L-fucosyl-(1,2)-D-ga lactose 3-N-acetyl-D-galactosaminyltransferase. Other names in common use include A-transferase, histo-blood group A glycosyltransferase, (Fucalpha1→2Galalpha1→3-N-acetylgalactosaminyltransferase), UDP-GalNAc:Fucalpha1→2Galalpha1→3-N-acetylgalactosaminyltransferase, alpha-3-N-acetylgalactosaminyltransferase, blood-group substance alpha-acetyltransferase, blood-group substance A-dependent acetylgalactosaminyltransferase, fucosylgalactose acetylgalactosaminyltransferase, histo-blood group A acetylgalactosaminyltransferase, histo-blood group A transferase, UDP-N-acetyl-D-galactosamine:alpha-L-fucosyl-1,2-D-galactose, and 3-N-acetyl-D-galactosaminyltransferase. This enzyme participates in 3 metabolic pathways: the lactoseries and neolactoseries of glycosphingolipid biosynthesis, as wellas the biosynthesis of glycan structures.

Structural studies

As of late 2007, 19 structures have been solved for this class of enzymes, with PDB accession codes 1LZ0, 1LZI, 1R7T, 1R7V, 1R7Y, 1R81, 1WSZ, 1WT0, 1WT1, 1WT2, 1WT3, 1XZ6, 1ZHJ, 1ZI1, 1ZI3, 1ZI4, 1ZI5, 1ZJO, and 2A8W.


Template:Enzyme references

  • Kobata A, Grollman EF, Ginsburg V (1968). "An enzymic basis for blood type A in humans". Arch. Biochem. Biophys. 124: 609–12. PMID 5661629.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  • Takeya A, Hosomi O, Ishiura M (Tokyo). "Complete purification and characterization of alpha-3-N-acetylgalactosaminyltransferase encoded by the human blood group A gene". J. Biochem.: 360–8. PMID 2341371. {{cite journal}}: Check date values in: |date= (help)CS1 maint: multiple names: authors list (link)
  • Yates AD, Feeney J, Donald AS, Watkins WM (1984). "Characterisation of a blood-group A-active tetrasaccharide synthesised by a blood-group B gene-specified glycosyltransferase". Carbohydr. Res. 130: 251–60. PMID 6434182.{{cite journal}}: CS1 maint: multiple names: authors list (link)

External links

The CAS registry number for this enzyme class is Template:CAS registry.

Template:Enzyme links

Gene Ontology (GO) codes

Template:GO code links

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Glycosyltransferase family 6 Provide feedback

No Pfam abstract.

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR005076

The biosynthesis of disaccharides, oligosaccharides and polysaccharides involves the action of hundreds of different glycosyltransferases. These enzymes catalyse the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. A classification of glycosyltransferases using nucleotide diphospho-sugar, nucleotide monophospho-sugar and sugar phosphates ([intenz:2.4.1.-]) and related proteins into distinct sequence based families has been described [ PUBMED:9334165 ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. The same three-dimensional fold is expected to occur within each of the families. Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence similarities may have similar 3-D structures and therefore form 'clans'.

Glycosyltransferase family 6 CAZY comprises enzymes with three known activities; alpha-1,3-galactosyltransferase ( EC ); alpha-1,3 N-acetylgalactosaminyltransferase ( EC ); alpha-galactosyltransferase ( EC ).

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

Loading domain graphics...


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
Jalview View  View  View  View  View  View  View 
HTML View  View           
PP/heatmap 1 View           

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

Representative proteomes UniProt

Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Representative proteomes UniProt
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_4383 (release 6.6)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A
Number in seed: 6
Number in full: 1872
Average length of the domain: 272 aa
Average identity of full alignment: 41 %
Average coverage of the sequence by the domain: 84.3 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 25.3 25.0
Noise cut-off 24.1 22.9
Model length: 313
Family (HMM) version: 16
Download: download the raw HMM for this family

Species distribution

Sunburst controls


Weight segments by...

Change the size of the sunburst


Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


Generate a FASTA-format file

Clear selection

This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Loading sunburst data...

Tree controls


The tree shows the occurrence of this domain across different species. More...


Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.


For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Glyco_transf_6 domain has been found. There are 245 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

Loading structure mapping...

AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A2R8Q6M0 View 3D Structure Click here
A0A4Z3 View 3D Structure Click here
A2AUQ7 View 3D Structure Click here
A2AUQ8 View 3D Structure Click here
A2BIR9 View 3D Structure Click here
A2CED1 View 3D Structure Click here
A2CED6 View 3D Structure Click here
B0R0W5 View 3D Structure Click here
B0R0W7 View 3D Structure Click here
B0S7I1 View 3D Structure Click here
D3ZNQ3 View 3D Structure Click here
D4A7T1 View 3D Structure Click here
E9PTA3 View 3D Structure Click here
F1QJ68 View 3D Structure Click here
G3V9Q9 View 3D Structure Click here
M0RE11 View 3D Structure Click here
P14769 View 3D Structure Click here
P16442 View 3D Structure Click here
P23336 View 3D Structure Click here
P38649 View 3D Structure Click here
P50127 View 3D Structure Click here
Q2NKH9 View 3D Structure Click here
Q2YDM8 View 3D Structure Click here
Q3L7M0 View 3D Structure Click here
Q3V1N9 View 3D Structure Click here
Q4R5T7 View 3D Structure Click here
Q5ZLK4 View 3D Structure Click here
Q7Z4J2 View 3D Structure Click here
Q8CFC4 View 3D Structure Click here
Q8HY56 View 3D Structure Click here
Q8N5D6 View 3D Structure Click here
Q8SPR2 View 3D Structure Click here
Q8SQ20 View 3D Structure Click here
Q8VI38 View 3D Structure Click here
Q95158 View 3D Structure Click here
Q9ET32 View 3D Structure Click here
U3KPV4 View 3D Structure Click here