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0  structures 201  species 0  interactions 290  sequences 1  architecture

Family: YopJ (PF03421)

Summary: YopJ Serine/Threonine acetyltransferase

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YopJ Serine/Threonine acetyltransferase Provide feedback

The Yersinia effector YopJ inhibits the innate immune response by blocking MAP kinase and NFkappaB signaling pathways. YopJ is a serine/threonine acetyltransferase which regulates signalling pathways by blocking phosphorylation [1]. Specifically, YopJ has been shown to block phosphorylation of active site residues [3]. It has also been shown that YopJ acetyltransferase is activated by eukaryotic host cell inositol hexakisphosphate [4]. This family was previously incorrectly annotated in Pfam as being a peptidase family.

Literature references

  1. Mukherjee S, Keitany G, Li Y, Wang Y, Ball HL, Goldsmith EJ, Orth K; , Science. 2006;312:1211-1214.: Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation. PUBMED:16728640 EPMC:16728640

  2. Yoon S, Liu Z, Eyobo Y, Orth K; , J Biol Chem. 2003;278:2131-2135.: Yersinia effector YopJ inhibits yeast MAPK signaling pathways by an evolutionarily conserved mechanism. PUBMED:12433923 EPMC:12433923

  3. Mittal R, Peak-Chew SY, McMahon HT;, Proc Natl Acad Sci U S A. 2006;103:18574-18579.: Acetylation of MEK2 and I kappa B kinase (IKK) activation loop residues by YopJ inhibits signaling. PUBMED:17116858 EPMC:17116858

  4. Mittal R, Peak-Chew SY, Sade RS, Vallis Y, McMahon HT;, J Biol Chem. 2010; [Epub ahead of print]: The acetyltransferase activity of the bacterial toxin YopJ of Yersinia is activated by eukaryotic host cell inositol hexakisphosphate. PUBMED:20430892 EPMC:20430892


External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR005083

The infection of mammalian host cells by Yersinia sp. causes a rapid induction of the mitogen-activated protein kinase (MAPK; including the ERK, JNK and p38 pathways) and nuclear factor kappaB (NF-kappaB) signalling pathways that would typically result in cytokine production and initiation of the innate immune response. However, these pathways are rapidly inhibited promoting apoptosis. YopJ has been shown to block phosphorylation of active site residues [PUBMED:17116858]. It has also been shown that YopJ acetyltransferase is activated by eukaryotic host cell inositol hexakisphosphate [PUBMED:20430892].

Serine and threonine acetylation is yet another complication to the control of signalling pathways and may be a may be a widespread mode of biochemical regulation of endogenous processes in eukaryotic cells. It has been shown that YopJ is a serine/threonine acetyltransferase [PUBMED:17412595]. It acetylates the serine and threonine residues in the phosphorylation sites of MAPK kinases and nuclear factor kappaB, preventing their activation by phosphorylation and the inhibition of these signalling pathways [PUBMED:17116858].

This entry contains YopJ and related proteins.

Domain organisation

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Alignments

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(17)
Full
(290)
Representative proteomes NCBI
(190)
Meta
(4)
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(2)
RP35
(8)
RP55
(16)
RP75
(29)
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  Seed
(17)
Full
(290)
Representative proteomes NCBI
(190)
Meta
(4)
RP15
(2)
RP35
(8)
RP55
(16)
RP75
(29)
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  Seed
(17)
Full
(290)
Representative proteomes NCBI
(190)
Meta
(4)
RP15
(2)
RP35
(8)
RP55
(16)
RP75
(29)
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

External links

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Curation and family details

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Seed source: Bateman A
Previous IDs: Peptidase_C55;
Type: Family
Author: Bateman A, Mistry J
Number in seed: 17
Number in full: 290
Average length of the domain: 171.80 aa
Average identity of full alignment: 37 %
Average coverage of the sequence by the domain: 54.49 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 19.9 19.9
Trusted cut-off 20.3 19.9
Noise cut-off 18.0 19.2
Model length: 178
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

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