Summary: uDENN domain
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Levivier E, Goud B, Souchet M, Calmels TP, Mornon JP, Callebaut I; , Biochem Biophys Res Commun 2001;287:688-695.: uDENN, DENN, and dDENN: indissociable domains in Rab and MAP kinases signaling pathways. PUBMED:11563850 EPMC:11563850
This tab holds annotation information from the InterPro database.
InterPro entry IPR005113
The tripartite DENN (after differentially expressed in neoplastic versus normal cells) domain is found in several proteins involved in Rab-mediated processes or regulation of MAPKs (Mitogen-activated preotein kinases) signaling pathways. It actually consists of three parts as the original DENN domain is always encircled on both sides by more divergent domains, called uDENN (after upstream DENN) and dDENN (for downstream DENN). The tripartite DENN domain is found associated with other domains, such as RUN, PLAT, PH, PPR, WD-40, GRAM or C1. The function of DENN domain remains to date unclear, although it appears to represent a good candidate for a GTP/GDP exchange activity [PUBMED:11563850, PUBMED:12906859].
The general characteristics of DENN domains - three regions dDENN, DENN itself, and uDENN having different patterns of sequence conservation and separated by sequences of variable length - suggest that they are composed of at least three sub-domains which may feature distinct folds but which are always associated due to functional and/or structural constraints [PUBMED:11563850].
Some proteins known to contain a tripartite DENN domain are listed below:
- Rat Rab3 GDP/GTP exchange protein (Rab3GEP)
- Human mitogen-activated protein kinase activating protein containing death domain (MADD). It is orthologous to Rab3GEP
- Caenorhabditis elegans regulator of presynaptic activity aex-3, the ortholog of Rab3GEP
- Mouse Rab6 interacting protein 1 (Rab6IP1)
- Human SET domain-binding factor 1(SBF1)
- Human suppressor of tumoreginicity 5 (ST5)
- Human C-MYC promoter-binding protein IRLB
This entry represents the uDENN domain.
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Curation and family details
|Seed source:||Callebaut I|
|Number in seed:||206|
|Number in full:||1772|
|Average length of the domain:||64.60 aa|
|Average identity of full alignment:||27 %|
|Average coverage of the sequence by the domain:||5.62 %|
|HMM build commands:||
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 11927849 -E 1000 --cpu 4 HMM pfamseq
|Family (HMM) version:||15|
|Download:||download the raw HMM for this family|
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There are 4 interactions for this family. More...
We determine these interactions using iPfam, which considers the interactions between residues in three-dimensional protein structures and maps those interactions back to Pfam families. You can find more information about the iPfam algorithm in the journal article that accompanies the website.
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the uDENN domain has been found. There are 2 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
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