Summary: Regulator of volume decrease after cellular swelling
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Regulator of volume decrease after cellular swelling Provide feedback
ICln is a ubiquitously expressed multi-functional protein that plays a critical role in regulating volume decrease in cells after cellular swelling. In plants, ICln induces Cl- currents [1,4,5], thus regulating Cl- homoeostasis in eukaryotes [2,3]. Structurally, the fold resembles a pleckstrin homology fold, on of whose roles is to recruit and tether their host protein to the cell membrane; and although the surface charges of the ICln fold are not equivalent to those of the PH domain, ICln can be phosphorylated in vitro and the PH-nature of the domain may be the part involving it in the transposition from cytosol to cell membrane during cytotonic swelling [1].
Literature references
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Furst J, Botta G, Saino S, Dopinto S, Gandini R, Dossena S, Vezzoli V, Rodighiero S, Bazzini C, Garavaglia ML, Meyer G, Jakab M, Ritter M, Wappl-Kornherr E, Paulmichl M;, Acta Physiol (Oxf). 2006;187:43-49.: The ICln interactome. PUBMED:16734741 EPMC:16734741
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Tamma G, Procino G, Strafino A, Bononi E, Meyer G, Paulmichl M, Formoso V, Svelto M, Valenti G;, Endocrinology. 2007;148:1118-1130.: Hypotonicity induces aquaporin-2 internalization and cytosol-to-membrane translocation of ICln in renal cells. PUBMED:17138647 EPMC:17138647
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Schmidt S, Jakab M, Costa I, Furst J, Ravasio A, Paulmichl M, Botta G, Ritter M;, Cell Physiol Biochem. 2009;23:397-406.: Quaternary structure assessment of ICln by fluorescence resonance energy transfer (FRET) in vivo. PUBMED:19471107 EPMC:19471107
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Brumos J, Talon M, Bouhlal R, Colmenero-Flores JM;, Plant Cell Environ. 2010;33:2012-2027.: Cl- homeostasis in includer and excluder citrus rootstocks: transport mechanisms and identification of candidate genes. PUBMED:20573047 EPMC:20573047
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Park JB, Son SJ, Lee GS, Cho PY, Song KS, Ryu PD, Kang SY, Hong SJ;, Mol Biochem Parasitol. 2005;140:197-203.: Molecular and electrophysiological characterization of nucleotide-sensitive chloride current-inducing protein of Fasciola hepatica. PUBMED:15760659 EPMC:15760659
Internal database links
SCOOP: | Vps36_ESCRT-II |
This tab holds annotation information from the InterPro database.
InterPro entry IPR039924
This entry includes ICln from animal and plants, and Lot5 from fungi. The function of Lot5 (low temperature responsive 5) is not known [PUBMED:11327734].
ICln, known as methylosome subunit pICln or chloride conductance regulatory protein ICln, owes these different names to its function in multiple regulatory pathways [PUBMED:16734741] as different as ion permeation, ribonucleoprotein biosynthesis and cytoskeletal organisation [PUBMED:9556550]. ICln can be identified both in the cytosol and in the cellular membrane, where it functions as a chloride current regulator and is important in regulating volume decrease after cellular swelling [PUBMED:20573047, PUBMED:15760659, PUBMED:17138647, PUBMED:19471107].
pLCln also functions as a Sm chaperone in the stepwise snRNP assembly process [PUBMED:11747828]. snRNPs is a RNA-protein complex esessential to the removal of introns from pre-mRNA [PUBMED:19520849, PUBMED:10330151]. In humans, the core of snRNPs is composed of seven Sm proteins bound to snRNA. pLCln tethers the hetero-oligomers SmD1/D2 and SmE/F/G into a ring-shaped 6S complex, which subsequently docks onto the SMN complex. The SMN complex then removes pICln and enables the transfer of pre-assembled Sm proteins onto snRNA [PUBMED:23333303]. Consistent with the role of human pICln, the orthologue from S. pombe is required for optimal production of the spliceosomal snRNPs and for efficient splicing [PUBMED:24298023].
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan PH (CL0266), which has the following description:
Members of this clan share a PH-like fold. Many families in this clan bind to short peptide motifs in proteins and are involved in signalling.
The clan contains the following 73 members:
ASK_PH BBL5 bPH_1 bPH_2 bPH_3 bPH_4 bPH_5 bPH_6 CARM1 Carm_PH DCP1 DUF1126 DUF1681 DUF3203 EbsA FERM_C Glycoprot_B_PH1 Glycoprot_B_PH2 GRAM hSac2 ICAP-1_inte_bdg INPP5B_PH IQ_SEC7_PH IRS ISP1_C ISP3_C Jak1_Phl Mcp5_PH Myosin_TH1 OCRL_clath_bd PH PH_10 PH_11 PH_12 PH_13 PH_14 PH_15 PH_16 PH_17 PH_18 PH_19 PH_2 PH_3 PH_4 PH_5 PH_6 PH_8 PH_9 PH_BEACH PH_RBD PH_TFIIH PID PID_2 POB3_N Proteasom_Rpn13 PTB Ran_BP1 Rtt106 SCAB-PH Sec3-PIP2_bind Sharpin_PH SIN1_PH SNX17_FERM_C SPT16 SSrecog SYCP2_SLD UCH_N VID27_PH Voldacs Vps36_ESCRT-II WH1 YcxB ZFYVE21_CAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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Seed (37) |
Full (1478) |
Representative proteomes | UniProt (2314) |
NCBI (2602) |
Meta (2) |
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RP15 (230) |
RP35 (612) |
RP55 (1037) |
RP75 (1470) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (37) |
Full (1478) |
Representative proteomes | UniProt (2314) |
NCBI (2602) |
Meta (2) |
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RP15 (230) |
RP35 (612) |
RP55 (1037) |
RP75 (1470) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
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Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
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Curation and family details
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Curation
Seed source: | PRINTS |
Previous IDs: | ICln_channel; |
Type: | Domain |
Sequence Ontology: | SO:0000417 |
Author: |
Griffiths-Jones SR |
Number in seed: | 37 |
Number in full: | 1478 |
Average length of the domain: | 125.80 aa |
Average identity of full alignment: | 25 % |
Average coverage of the sequence by the domain: | 51.12 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 141 | ||||||||||||
Family (HMM) version: | 14 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Voldacs domain has been found. There are 23 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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