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0  structures 2653  species 0  interactions 8804  sequences 21  architectures

Family: Na_H_antiporter (PF03553)

Summary: Na+/H+ antiporter family

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This is the Wikipedia entry entitled "Sodium–hydrogen antiporter". More...

Sodium–hydrogen antiporter Edit Wikipedia article

The sodium–hydrogen antiporter or sodium–proton exchanger (Na+/H+ exchanger) is a membrane protein that transports Na+ into the cell, and H+ out of the cell (antiport).

Na+/H+ antiporter 1
1zcd opm.png
Identifiers
SymbolNa_H_antiport_1
PfamPF06965
InterProIPR004670
TCDB2.A.36
OPM superfamily106
OPM protein1zcd

Function

They are found in the membranes of many cells, and especially in those of the nephron of the kidney, specifically in the intercalary cells of the collecting duct and in the epithelial cells of the proximal convoluted tubule. The membrane pump is primarily responsible for maintaining homeostasis of pH and sodium.[1] Defects in Na+/H+ antiporters may result in heart or kidney failure.[2] Angiotensin II upregulates this antiporter in the proximal convoluted tubule in order to promote Na+ reabsorption and H+ secretion. Na+/H+ exchangers are thought to be implicated in other disorders such as hypertension. In one study, transgenic mice over expressing this membrane protein were shown to have increased reabsorption and retention of sodium after increased salt intake.[3]

In dopamine receptor signalling,[4] the widely expressed Na+/H+ exchanger NHE-1 is activated downstream of the D2,[5] D3,[6][7] and D4 receptors.[8]

Isoforms

There are several isoforms of the antiporter:

Families

There are several families of sodium/proton antiporters that facilitate the exchange of sodium ions with protons across the lipid membrane. Some of them include:[9]

References

  1. ^ Padan E, Landau M (2016). "Chapter 12: Sodium-Proton (Na+/H+) Antiporters:Properties and Roles in Health and Disease". In Sigel A, Sigel H, Sigel RK (eds.). The Alkali Metal Ions: Their Role in Life. Metal Ions in Life Sciences. 16. Springer. pp. 391–458. doi:10.1007/978-4-319-21756-7_12. ISBN 978-3-319-21755-0.
  2. ^ Bobulescu IA, Moe OW (September 2006). "Na+/H+ exchangers in renal regulation of acid-base balance". Seminars in Nephrology. 26 (5): 334–44. doi:10.1016/j.semnephrol.2006.07.001. PMC 2878276. PMID 17071327.
  3. ^ Kuro-o, M (January 1995). "Salt-sensitive hypertension in transgenic mice overexpressing Na(+)-proton exchanger". Circulation Research. doi:10.1161/01.res.76.1.148.
  4. ^ Yun CH, Tse CM, Nath SK, Levine SA, Brant SR, Donowitz M (July 1995). "Mammalian Na+/H+ exchanger gene family: structure and function studies". The American Journal of Physiology. 269 (1 Pt 1): G1–11. doi:10.1152/ajpgi.1995.269.1.G1. PMID 7631785.
  5. ^ Neve KA, Kozlowski MR, Rosser MP (December 1992). "Dopamine D2 receptor stimulation of Na+/H+ exchange assessed by quantification of extracellular acidification". The Journal of Biological Chemistry. 267 (36): 25748–53. PMID 1361188.
  6. ^ Cox BA, Rosser MP, Kozlowski MR, Duwe KM, Neve RL, Neve KA (September 1995). "Regulation and functional characterization of a rat recombinant dopamine D3 receptor". Synapse. 21 (1): 1–9. doi:10.1002/syn.890210102. PMID 8525456.
  7. ^ Chio CL, Lajiness ME, Huff RM (January 1994). "Activation of heterologously expressed D3 dopamine receptors: comparison with D2 dopamine receptors". Molecular Pharmacology. 45 (1): 51–60. PMID 8302280.
  8. ^ Chio CL, Drong RF, Riley DT, Gill GS, Slightom JL, Huff RM (April 1994). "D4 dopamine receptor-mediated signaling events determined in transfected Chinese hamster ovary cells". The Journal of Biological Chemistry. 269 (16): 11813–9. PMID 7512953.
  9. ^ "TCDB Â» HOME". Transporter Classification Database. Retrieved 2016-03-14.

External links

This article incorporates text from the public domain Pfam and InterPro: IPR004670


This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Na+/H+ antiporter family Provide feedback

This family includes integral membrane proteins, some of which are NA+/H+ antiporters [1].

Literature references

  1. Ito M, Guffanti AA, Zemsky J, Ivey DM, Krulwich TA; , J Bacteriol 1997;179:3851-3857.: Role of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus OF4. PUBMED:9190799 EPMC:9190799


Internal database links

External database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR018461

This entry represents the C-terminal domain of a group of integral membrane proteins, some of which are NA+/H+ antiporters [PUBMED:9190799].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan IT (CL0182), which has the following description:

This superfamily of secondary carriers specific for cationic and anionic compounds, has been termed the ion transporter (IT) superfamily [1].

The clan contains the following 19 members:

ABG_transport ArsB CitMHS CitMHS_2 DctM DcuA_DcuB DcuC DUF1646 DUF401 EXS GntP_permease Lactate_perm MatC_N Na_H_antiport_2 Na_H_antiport_3 Na_H_antiporter Na_sulph_symp NhaB SCFA_trans

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(32)
Full
(8804)
Representative proteomes UniProt
(43692)
NCBI
(73630)
Meta
(748)
RP15
(1017)
RP35
(4209)
RP55
(8706)
RP75
(15371)
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PP/heatmap 1                

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(32)
Full
(8804)
Representative proteomes UniProt
(43692)
NCBI
(73630)
Meta
(748)
RP15
(1017)
RP35
(4209)
RP55
(8706)
RP75
(15371)
Alignment:
Format:
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Sequence:
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(32)
Full
(8804)
Representative proteomes UniProt
(43692)
NCBI
(73630)
Meta
(748)
RP15
(1017)
RP35
(4209)
RP55
(8706)
RP75
(15371)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_620 (release 7.0)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Bateman A
Number in seed: 32
Number in full: 8804
Average length of the domain: 255.90 aa
Average identity of full alignment: 17 %
Average coverage of the sequence by the domain: 73.88 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.0 23.0
Trusted cut-off 23.0 23.0
Noise cut-off 22.9 22.9
Model length: 303
Family (HMM) version: 15
Download: download the raw HMM for this family

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