Summary: Organic solute transporter Ostalpha
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Organic solute transporter Ostalpha Provide feedback
This family is a transmembrane organic solute transport protein. In vertebrates these proteins form a complex with Ostbeta, and function as bile transporters [1]. In plants they may transport brassinosteroid-like compounds and act as regulators of cell death [2].
Literature references
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Dawson PA, Hubbert M, Haywood J, Craddock AL, Zerangue N, Christian WV, Ballatori N;, J Biol Chem. 2005;280:6960-6968.: The heteromeric organic solute transporter alpha-beta, Ostalpha-Ostbeta, is an ileal basolateral bile acid transporter. PUBMED:15563450 EPMC:15563450
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Malinovsky FG, Brodersen P, Fiil BK, McKinney LV, Thorgrimsen S, Beck M, Nielsen HB, Pietra S, Zipfel C, Robatzek S, Petersen M, Hofius D, Mundy J;, PLoS One. 2010;5:e12586.: Lazarus1, a DUF300 protein, contributes to programmed cell death associated with arabidopsis acd11 and the hypersensitive response. PUBMED:20830211 EPMC:20830211
This tab holds annotation information from the InterPro database.
InterPro entry IPR005178
This entry includes Organic solute transporter subunit alpha (OSTalpha, also known as SLC51A) and Transmembrane protein 184 (TMEM184).
Ost-alpha protein is a 7-transmembrane (TM) domain containing protein that forms a transporter complex with Ost-beta protein, which is a single-TM domain polypeptide. This heterodimerisation is required for the delivery of the complex to the plasma membrane. The OSTalpha-OSTbeta complex serves as a multispecific transporter that may participate in cellular uptake of bile acids, some endogenous and exogenous steroids, and eicosanoids. It functions via a facilitated diffusion mechanism. Interestingly, this transporter also transports dehydroepiandrosterone sulfate (DHEAS) and pregnenolone sulfate (PREGS), which are major excitatory neurosteroids. This suggests a possible function for OSTalpha-OSTbeta complex in the brain [ PUBMED:23506901 ]. In plants this complex may transport brassinosteroid-like compounds and act as regulators of cell death [ PUBMED:20830211 ].
Human TMEM184C is a possible tumour suppressor which may play a role in cell growth [ PUBMED:17072649 ]. This entry also includes Arabidopsis protein LAZ1, which is required for programmed cell death (PCD) associated with hypersensitive response (HR) [ PUBMED:20830211 ].
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan GPCR_A (CL0192), which has the following description:
This clan contains various seven-transmembrane receptors and related proteins. A major member is Pfam:PF00001, members of which have been considered to be typical members of the rhodopsin superfamily. Many members of this clan are Caenorhabditis proteins, suggesting great expansion of the relevant families in these nematode worms.
The clan contains the following 53 members:
7TM-7TMR_HD 7tm_1 7tm_2 7tm_3 7tm_4 7TM_GPCR_Sra 7TM_GPCR_Srab 7TM_GPCR_Srb 7TM_GPCR_Srbc 7TM_GPCR_Srd 7TM_GPCR_Srh 7TM_GPCR_Sri 7TM_GPCR_Srj 7TM_GPCR_Srsx 7TM_GPCR_Srt 7TM_GPCR_Sru 7TM_GPCR_Srv 7TM_GPCR_Srw 7TM_GPCR_Srx 7TM_GPCR_Srz 7TM_GPCR_Str 7TMR-DISM_7TM Bac_rhodopsin Ceramidase Chs7 Dicty_CAR DUF1182 DUF3522 DUF621 Frizzled Git3 GpcrRhopsn4 GPR_Gpa2_C Heliorhodopsin HisKA_7TM HlyIII Lung_7-TM_R MASE3 MASE4 Ocular_alb Per1 Pombe_5TM Serpentine_r_xa SID-1_RNA_chan Solute_trans_a Sre Srg STE3 TAS2R THH1_TOM1-3_dom TMEM187 Tmemb_40 V1RAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (263) |
Full (7010) |
Representative proteomes | UniProt (11577) |
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RP15 (1349) |
RP35 (3180) |
RP55 (5580) |
RP75 (7296) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
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Seed (263) |
Full (7010) |
Representative proteomes | UniProt (11577) |
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RP15 (1349) |
RP35 (3180) |
RP55 (5580) |
RP75 (7296) |
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Raw Stockholm | |||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_3382 (release 7.0) |
Previous IDs: | DUF300; |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Finn RD |
Number in seed: | 263 |
Number in full: | 7010 |
Average length of the domain: | 244.3 aa |
Average identity of full alignment: | 28 % |
Average coverage of the sequence by the domain: | 58.13 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 243 | ||||||||||||
Family (HMM) version: | 19 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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AlphaFold Structure Predictions
The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.
trRosetta Structure
The structural model below was generated by the Baker group with the trRosetta software using the Pfam UniProt multiple sequence alignment.
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