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0  structures 1487  species 0  interactions 7010  sequences 101  architectures

Family: Solute_trans_a (PF03619)

Summary: Organic solute transporter Ostalpha

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Organic solute transporter Ostalpha Provide feedback

This family is a transmembrane organic solute transport protein. In vertebrates these proteins form a complex with Ostbeta, and function as bile transporters [1]. In plants they may transport brassinosteroid-like compounds and act as regulators of cell death [2].

Literature references

  1. Dawson PA, Hubbert M, Haywood J, Craddock AL, Zerangue N, Christian WV, Ballatori N;, J Biol Chem. 2005;280:6960-6968.: The heteromeric organic solute transporter alpha-beta, Ostalpha-Ostbeta, is an ileal basolateral bile acid transporter. PUBMED:15563450 EPMC:15563450

  2. Malinovsky FG, Brodersen P, Fiil BK, McKinney LV, Thorgrimsen S, Beck M, Nielsen HB, Pietra S, Zipfel C, Robatzek S, Petersen M, Hofius D, Mundy J;, PLoS One. 2010;5:e12586.: Lazarus1, a DUF300 protein, contributes to programmed cell death associated with arabidopsis acd11 and the hypersensitive response. PUBMED:20830211 EPMC:20830211


This tab holds annotation information from the InterPro database.

InterPro entry IPR005178

This entry includes Organic solute transporter subunit alpha (OSTalpha, also known as SLC51A) and Transmembrane protein 184 (TMEM184).

Ost-alpha protein is a 7-transmembrane (TM) domain containing protein that forms a transporter complex with Ost-beta protein, which is a single-TM domain polypeptide. This heterodimerisation is required for the delivery of the complex to the plasma membrane. The OSTalpha-OSTbeta complex serves as a multispecific transporter that may participate in cellular uptake of bile acids, some endogenous and exogenous steroids, and eicosanoids. It functions via a facilitated diffusion mechanism. Interestingly, this transporter also transports dehydroepiandrosterone sulfate (DHEAS) and pregnenolone sulfate (PREGS), which are major excitatory neurosteroids. This suggests a possible function for OSTalpha-OSTbeta complex in the brain [ PUBMED:23506901 ]. In plants this complex may transport brassinosteroid-like compounds and act as regulators of cell death [ PUBMED:20830211 ].

Human TMEM184C is a possible tumour suppressor which may play a role in cell growth [ PUBMED:17072649 ]. This entry also includes Arabidopsis protein LAZ1, which is required for programmed cell death (PCD) associated with hypersensitive response (HR) [ PUBMED:20830211 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan GPCR_A (CL0192), which has the following description:

This clan contains various seven-transmembrane receptors and related proteins. A major member is Pfam:PF00001, members of which have been considered to be typical members of the rhodopsin superfamily. Many members of this clan are Caenorhabditis proteins, suggesting great expansion of the relevant families in these nematode worms.

The clan contains the following 53 members:

7TM-7TMR_HD 7tm_1 7tm_2 7tm_3 7tm_4 7TM_GPCR_Sra 7TM_GPCR_Srab 7TM_GPCR_Srb 7TM_GPCR_Srbc 7TM_GPCR_Srd 7TM_GPCR_Srh 7TM_GPCR_Sri 7TM_GPCR_Srj 7TM_GPCR_Srsx 7TM_GPCR_Srt 7TM_GPCR_Sru 7TM_GPCR_Srv 7TM_GPCR_Srw 7TM_GPCR_Srx 7TM_GPCR_Srz 7TM_GPCR_Str 7TMR-DISM_7TM Bac_rhodopsin Ceramidase Chs7 Dicty_CAR DUF1182 DUF3522 DUF621 Frizzled Git3 GpcrRhopsn4 GPR_Gpa2_C Heliorhodopsin HisKA_7TM HlyIII Lung_7-TM_R MASE3 MASE4 Ocular_alb Per1 Pombe_5TM Serpentine_r_xa SID-1_RNA_chan Solute_trans_a Sre Srg STE3 TAS2R THH1_TOM1-3_dom TMEM187 Tmemb_40 V1R

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(263)
Full
(7010)
Representative proteomes UniProt
(11577)
RP15
(1349)
RP35
(3180)
RP55
(5580)
RP75
(7296)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(263)
Full
(7010)
Representative proteomes UniProt
(11577)
RP15
(1349)
RP35
(3180)
RP55
(5580)
RP75
(7296)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(263)
Full
(7010)
Representative proteomes UniProt
(11577)
RP15
(1349)
RP35
(3180)
RP55
(5580)
RP75
(7296)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_3382 (release 7.0)
Previous IDs: DUF300;
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD , Eberhardt R
Number in seed: 263
Number in full: 7010
Average length of the domain: 244.3 aa
Average identity of full alignment: 28 %
Average coverage of the sequence by the domain: 58.13 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 24.9 24.9
Trusted cut-off 25.0 24.9
Noise cut-off 24.7 24.7
Model length: 243
Family (HMM) version: 19
Download: download the raw HMM for this family

Species distribution

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Selections

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044R2G0 View 3D Structure Click here
A0A044TQS4 View 3D Structure Click here
A0A044VHX7 View 3D Structure Click here
A0A077ZAA3 View 3D Structure Click here
A0A077ZEN3 View 3D Structure Click here
A0A077ZKJ6 View 3D Structure Click here
A0A0D2DF20 View 3D Structure Click here
A0A0D2GJG0 View 3D Structure Click here
A0A0D2GLE1 View 3D Structure Click here
A0A0D2HMY9 View 3D Structure Click here
A0A0G2K0C2 View 3D Structure Click here
A0A0K0DVA2 View 3D Structure Click here
A0A0K0E0L3 View 3D Structure Click here
A0A0K0E9Y4 View 3D Structure Click here
A0A0K0EGE8 View 3D Structure Click here
A0A0K0EPD8 View 3D Structure Click here
A0A0K0JLQ4 View 3D Structure Click here
A0A0N4U2H1 View 3D Structure Click here
A0A0N4UHS3 View 3D Structure Click here
A0A0N4UJI3 View 3D Structure Click here
A0A0R0FLR3 View 3D Structure Click here
A0A0R4IMB8 View 3D Structure Click here
A0A0R4IXB8 View 3D Structure Click here
A0A175W7T8 View 3D Structure Click here
A0A183XZ18 View 3D Structure Click here
A0A1C1CLV2 View 3D Structure Click here
A0A1C1CPE7 View 3D Structure Click here
A0A1C1CYE7 View 3D Structure Click here
A0A1D6E388 View 3D Structure Click here
A0A1D6EWJ9 View 3D Structure Click here
A0A1D6ICF7 View 3D Structure Click here
A0A1D6K5D8 View 3D Structure Click here
A0A1D6K7D6 View 3D Structure Click here
A0A1D6LAB5 View 3D Structure Click here
A0A1D6LMQ4 View 3D Structure Click here
A0A1D8PK78 View 3D Structure Click here
A0A1P8B7M3 View 3D Structure Click here
A0A2R8Q819 View 3D Structure Click here
A0A2R8Q9M9 View 3D Structure Click here
A0A2R8QBQ3 View 3D Structure Click here

trRosetta Structure

The structural model below was generated by the Baker group with the trRosetta software using the Pfam UniProt multiple sequence alignment.

The InterPro website shows the contact map for the Pfam SEED alignment. Hovering or clicking on a contact position will highlight its connection to other residues in the alignment, as well as on the 3D structure.

Improved protein structure prediction using predicted inter-residue orientations. Jianyi Yang, Ivan Anishchenko, Hahnbeom Park, Zhenling Peng, Sergey Ovchinnikov, David Baker Proceedings of the National Academy of Sciences Jan 2020, 117 (3) 1496-1503; DOI: 10.1073/pnas.1914677117;