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65  structures 1801  species 0  interactions 4615  sequences 65  architectures

Family: Fumble (PF03630)

Summary: Fumble

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This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Fumble Provide feedback

Fumble is required for cell division in Drosophila. Mutants lacking fumble exhibit abnormalities in bipolar spindle organisation, chromosome segregation, and contractile ring formation. Analyses have demonstrated that encodes three protein isoforms, all of which contain a domain with high similarity to the pantothenate kinases of A. nidulans and [1]. A role of fumble in membrane synthesis has been [1].

Literature references

  1. Afshar K, Gonczy P, DiNardo S, Wasserman SA; , Genetics 2001;157:1267-1276.: fumble encodes a pantothenate kinase homolog required for proper mitosis and meiosis in Drosophila melanogaster. PUBMED:11238410 EPMC:11238410

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR004567

This family describes the type II form of pantothenate kinase PanK, characterised from the fungus Emericella nidulans and with similar forms known in several other eukaryotes. It also includes forms from several Gram-positive bacteria suggested to have originated from the eukaryotic form by lateral transfer. It differs in a number of biochemical properties (such as inhibition by acetyl-CoA) from type I PanK enzymes and shows little sequence similarity [ PUBMED:9890959 , PUBMED:12760898 ].

Pantothenate kinase (PanK or CoaA) catalyses the first step of the universal five step coenzyme A (CoA) biosynthesis pathway. CoA is a ubiquitous and essential cofactor in all living organsims. Pantothenate kinase catalyses the first and rate limiting step in the CoA biosynthetic pathway, which involves transferring a phosphoryl group from ATP to pantothenate, also known as vitamin B5. Three distinct types of pantothenate kinase enzymes have been identified: type I PanK enzymes are typified by the E. coli CoaA protein, type II enzymes are primarily found in eukaryotic organisms whilst type III enzymes have a wider phylogenic distribution and are not feedback inhibited by CoA [ PUBMED:18186650 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan Actin_ATPase (CL0108), which has the following description:

The actin-like ATPase domain forms an alpha/beta canonical fold. The domain can be subdivided into 1A, 1B, 2A and 2B subdomains. Subdomains 1A and 1B share the same RNAseH-like fold (a five-stranded beta-sheet decorated by a number of alpha-helices). Domains 1A and 2A are conserved in all members of this superfamily, whereas domain 1B and 2B have a variable structure and are even missing from some homologues [1]. Within the actin-like ATPase domain the ATP-binding site is highly conserved. The phosphate part of the ATP is bound in a cleft between subdomains 1A and 2A, whereas the adenosine moiety is bound to residues from domains 2A and 2B[1].

The clan contains the following 34 members:

Acetate_kinase Actin Actin_micro ALP_N AnmK BcrAD_BadFG Carbam_trans_N DDR DUF1464 DUF2229 EutA FGGY_C FGGY_N FtsA Fumble GDA1_CD39 Glucokinase Hexokinase_1 Hexokinase_2 HGD-D HSP70 Hydant_A_N Hydantoinase_A HypF_C MreB_Mbl MutL Pan_kinase PilM_2 Ppx-GppA RACo_C_ter ROK StbA T2SSL TsaD


We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

Representative proteomes UniProt
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1Cannot generate PP/Heatmap alignments for seeds; no PP data available

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Representative proteomes UniProt

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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

Representative proteomes UniProt
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...


This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_3299 (release 7.0)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Finn RD
Number in seed: 110
Number in full: 4615
Average length of the domain: 296.8 aa
Average identity of full alignment: 40 %
Average coverage of the sequence by the domain: 63.86 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 28.5 28.5
Trusted cut-off 29.2 28.5
Noise cut-off 28.4 28.0
Model length: 333
Family (HMM) version: 17
Download: download the raw HMM for this family

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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The tree shows the occurrence of this domain across different species. More...


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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Fumble domain has been found. There are 65 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A044TP91 View 3D Structure Click here
A0A077Z269 View 3D Structure Click here
A0A0D2EY82 View 3D Structure Click here
A0A0K0EQZ7 View 3D Structure Click here
A0A0N4UC21 View 3D Structure Click here
A0A0R0H094 View 3D Structure Click here
A0A0R0IZX1 View 3D Structure Click here
A0A0R4IK68 View 3D Structure Click here
A0A1C1D2W9 View 3D Structure Click here
A0A1D6I9S9 View 3D Structure Click here
A0A1D8PQ17 View 3D Structure Click here
A0A1I9G4R9 View 3D Structure Click here
A0A2R8Q6D9 View 3D Structure Click here
A0A3Q0KGZ2 View 3D Structure Click here
A4I387 View 3D Structure Click here
B6SPI3 View 3D Structure Click here
B8A4W8 View 3D Structure Click here
C0NLU7 View 3D Structure Click here
C1GZA8 View 3D Structure Click here
D3ZUQ7 View 3D Structure Click here
D3ZWD3 View 3D Structure Click here
F1LT70 View 3D Structure Click here
F1QEN2 View 3D Structure Click here
G4VQ30 View 3D Structure Click here
H2KYP7 View 3D Structure Click here
I1MTH1 View 3D Structure Click here
I1N1H5 View 3D Structure Click here
K7KMQ8 View 3D Structure Click here
K7KVS7 View 3D Structure Click here
O74962 View 3D Structure Click here
O80765 View 3D Structure Click here
Q04430 View 3D Structure Click here
Q08DA5 View 3D Structure Click here
Q0J035 View 3D Structure Click here
Q18580 View 3D Structure Click here
Q2FWC7 View 3D Structure Click here
Q384T6 View 3D Structure Click here
Q49Z76 View 3D Structure Click here
Q4DHB2 View 3D Structure Click here
Q4DME2 View 3D Structure Click here