Summary: Mitochondrial pyruvate carriers
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Mitochondrial pyruvate carriers Provide feedback
This family includes mitochondrial pyruvate carrier proteins, such as Mpc 1/2 and their homologues. They mediate the uptake of pyruvate into mitochondria [1]. In humans, Mpc1 and Mpc2 associate to form an ~150-kilodalton complex in the inner mitochondrial membrane [2]. MPC1 contains two predicted transmembrane regions. MPC2 contains three predicted transmembrane regions. Several studies have demonstrated MPC involvement in the control of cell fate in cancer and gluconeogenesis in models of type 2 diabetes [3].
Literature references
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Bricker DK, Taylor EB, Schell JC, Orsak T, Boutron A, Chen YC, Cox JE, Cardon CM, Van Vranken JG, Dephoure N, Redin C, Boudina S, Gygi SP, Brivet M, Thummel CS, Rutter J;, Science. 2012;337:96-100.: A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans. PUBMED:22628558 EPMC:22628558
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Bricker DK, Taylor EB, Schell JC, Orsak T, Boutron A, Chen YC, Cox JE, Cardon CM, Van Vranken JG, Dephoure N, Redin C, Boudina S, Gygi SP, Brivet M, Thummel CS, Rutter J;, Science. 2012;337:96-100.: A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans. PUBMED:22628558 EPMC:22628558
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Gray LR, Rauckhorst AJ, Taylor EB;, J Biol Chem. 2016;291:7409-7417.: A Method for Multiplexed Measurement of Mitochondrial Pyruvate Carrier Activity. PUBMED:26823462 EPMC:26823462
This tab holds annotation information from the InterPro database.
InterPro entry IPR005336
This entry represents the mitochondrial pyruvate carrier proteins, including Mpc 1/2 and their homologues. They mediate the uptake of pyruvate into mitochondria [PUBMED:22628558]. In humans, Mpc1 and Mpc2 associate to form an ~150-kilodalton complex in the inner mitochondrial membrane [PUBMED:22628558].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Cellular component | mitochondrial inner membrane (GO:0005743) |
Biological process | mitochondrial pyruvate transmembrane transport (GO:0006850) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan MtN3-like (CL0141), which has the following description:
The clan forms a large and diverse family of proteins with seven transmembrane helices, common topology and, most likely, similar function. Their coding genes exist in all eukaryota and in several prokaryota. Some are responsible for metabolic diseases (cystinosis, congenital disorder of glycosylation), others are candidate genes for genetic disorders (cleft lip and palate, certain forms of cancer) or solute uptake and efflux (SWEETs) and many have not yet been assigned a function. Comparison with the properties of well-annotated clan members suggests that the proteins could be involved in protein trafficking and serve as cargo receptors in vesicle trafficking [3].
The clan contains the following 5 members:
ER_lumen_recept LAB_N MPC MtN3_slv PQ-loopAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (44) |
Full (2583) |
Representative proteomes | UniProt (3575) |
NCBI (3937) |
Meta (7) |
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RP15 (700) |
RP35 (1460) |
RP55 (2060) |
RP75 (2529) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
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not generated,
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (44) |
Full (2583) |
Representative proteomes | UniProt (3575) |
NCBI (3937) |
Meta (7) |
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RP15 (700) |
RP35 (1460) |
RP55 (2060) |
RP75 (2529) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | SWISS-PROT |
Previous IDs: | UPF0041; |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Bateman A |
Number in seed: | 44 |
Number in full: | 2583 |
Average length of the domain: | 100.70 aa |
Average identity of full alignment: | 36 % |
Average coverage of the sequence by the domain: | 72.09 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 110 | ||||||||||||
Family (HMM) version: | 13 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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