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19  structures 1126  species 0  interactions 1863  sequences 79  architectures

Family: Not1 (PF04054)

Summary: CCR4-Not complex component, Not1

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "CNOT1". More...

CNOT1 Edit Wikipedia article

Available structures
PDBOrtholog search: PDBe RCSB
AliasesCNOT1, CDC39, NOT1, NOT1H, AD-005, CCR4-NOT transcription complex subunit 1, HPE12
External IDsOMIM: 604917 MGI: 2442402 HomoloGene: 9453 GeneCards: CNOT1
Gene location (Human)
Chromosome 16 (human)
Chr.Chromosome 16 (human)[1]
Chromosome 16 (human)
Genomic location for CNOT1
Genomic location for CNOT1
Band16q21Start58,519,951 bp[1]
End58,629,885 bp[1]
RNA expression pattern
PBB GE CNOT1 200860 s at fs.png

PBB GE CNOT1 200861 at fs.png
More reference expression data
RefSeq (mRNA)



RefSeq (protein)



Location (UCSC)Chr 16: 58.52 – 58.63 MbChr 8: 95.72 – 95.81 Mb
PubMed search[3][4]
View/Edit HumanView/Edit Mouse
CCR4-Not complex component, Not1

CCR4-NOT transcription complex subunit 1 is a protein that in humans is encoded by the CNOT1 gene.[5][6]

It is a part of the Ccr4-Not complex, which deadenylates mRNAs.[7] CNOT1 acts as a scaffold protein, binding other subunits of the complex.[8][9][10]


CNOT1 has been shown to interact with CNOT8.[11][12]


  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000125107 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000036550 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, et al. (January 2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nature Genetics. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
  6. ^ "Entrez Gene: CNOT1 CCR4-NOT transcription complex, subunit 1".
  7. ^ Garneau NL, Wilusz J, Wilusz CJ (Feb 2007). "The highways and byways of mRNA decay". Nat. Rev. Mol. Cell Biol. 8 (2): 113–26. doi:10.1038/nrm2104. PMID 17245413.
  8. ^ Sandler H, Kreth J, Timmers HT, Stoecklin G (May 2011). "Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin". Nucleic Acids Res. 39 (10): 4373–86. doi:10.1093/nar/gkr011. PMC 3105394. PMID 21278420.
  9. ^ Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O (Nov 2012). "The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex". Nucleic Acids Res. 40 (21): 11058–72. doi:10.1093/nar/gks883. PMC 3510486. PMID 22977175.
  10. ^ Basquin J, Roudko VV, Rode M, Basquin C, Séraphin B, Conti E (Oct 26, 2012). "Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction". Mol. Cell. 48 (2): 207–18. doi:10.1016/j.molcel.2012.08.014. PMID 22959269.
  11. ^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
  12. ^ Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT (February 2000). "Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits". Nucleic Acids Res. 28 (3): 809–17. doi:10.1093/nar/28.3.809. PMC 102560. PMID 10637334.

Further reading

External links

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

CCR4-Not complex component, Not1 Provide feedback

The Ccr4-Not complex is a global regulator of transcription that affects genes positively and negatively and is thought to regulate transcription factor TFIID [1].

Literature references

  1. Maillet L, Collart MA; , J Biol Chem 2002;277:2835-2842.: Interaction between Not1p, a component of the Ccr4-not complex, a global regulator of transcription, and Dhh1p, a putative RNA helicase. PUBMED:11696541 EPMC:11696541

This tab holds annotation information from the InterPro database.

InterPro entry IPR007196

The Ccr4-Not complex is a global regulator of gene expression that is conserved from yeast to human. It affects genes positively and negatively and is thought to regulate transcription factor IID function. In Saccharomyces cerevisiae, it exists in two prominent forms and consists of at least nine core subunits: the five Not proteins (Not1 to Not5), Caf1, Caf40, Caf130 and Ccr4 [PUBMED:10637334]. The Ccr4-Not complex regulates many different cellular functions, including RNA degradation and transcription initiation. It may be a regulatory platform that senses nutrient levels and stress [PUBMED:12957374]. Caf1 and Ccr4, are directly involved in mRNA deadenylation, and Caf1p is associated with Dhh1, a putative RNA helicase thought to be a component of the decapping complex [PUBMED:11696541]. Pop2, a component of the Ccr4-Not complex, functions as a deadenylase [PUBMED:18430587].

The Ccr4-Not complex is a global regulator of transcription that affects genes positively and negatively and is thought to regulate transcription factor TFIID [PUBMED:11696541].

Domain organisation

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Seed source: Pfam-B_13503 (release 7.3);
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Wood V , Finn RD
Number in seed: 102
Number in full: 1863
Average length of the domain: 336.00 aa
Average identity of full alignment: 43 %
Average coverage of the sequence by the domain: 16.85 %

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HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 22.7 22.7
Trusted cut-off 22.7 24.6
Noise cut-off 21.3 21.5
Model length: 369
Family (HMM) version: 16
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Species distribution

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For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Not1 domain has been found. There are 19 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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