Summary: Isoprenylcysteine carboxyl methyltransferase (ICMT) family
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Isoprenylcysteine carboxyl methyltransferase (ICMT) family Provide feedback
The isoprenylcysteine o-methyltransferase ( EC:2.1.1.100) family carry out carboxyl methylation of cleaved eukaryotic proteins that terminate in a CaaX motif. In Saccharomyces cerevisiae this methylation is carried out by Ste14p, an integral endoplasmic reticulum membrane protein. Ste14p is the founding member of the isoprenylcysteine carboxyl methyltransferase (ICMT) family, whose members share significant sequence homology [1].
Literature references
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Romano JD, Michaelis S; , Mol Biol Cell 2001;12:1957-1971.: Topological and mutational analysis of Saccharomyces cerevisiae Ste14p, founding member of the isoprenylcysteine carboxyl methyltransferase family. PUBMED:11451995 EPMC:11451995
Internal database links
SCOOP: | DUF1295 ERG4_ERG24 NnrU PEMT Steroid_dh |
Similarity to PfamA using HHSearch: | PEMT DUF1295 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR007269
The isoprenylcysteine o-methyltransferase (EC) carries out carboyxl methylation of cleaved eukaryotic proteins that terminate in a CaaX motif. In Saccharomyces cerevisiae, this methylation is carried out by Ste14p, an integral endoplasmic reticulum membrane protein. Ste14p is the founding member of the isoprenylcysteine carboxyl methyltransferase (ICMT) family, whose members share significant sequence homology [PUBMED:11451995].
This entry also includes ICMT from Methanosarcina acetivorans (SWISSPROT). It comprises a core of five transmembrane alpha helices and a cofactor-binding pocket enclosed within a highly conserved C-terminal catalytic subdomain [PUBMED:22195972].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Cellular component | integral component of membrane (GO:0016021) |
Molecular function | protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity (GO:0004671) |
Biological process | C-terminal protein methylation (GO:0006481) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan Steroid_dh (CL0115), which has the following description:
This clan includes several enzymes, including steroid dehydrogenases and isoprenylcysteine carboxyl methyltransferase enzymes. These protein contain a varying number of transmembrane regions.
The clan contains the following 6 members:
DUF1295 ERG4_ERG24 ICMT NnrU PEMT Steroid_dhAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (12) |
Full (3744) |
Representative proteomes | UniProt (11384) |
NCBI (37745) |
Meta (1174) |
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RP15 (598) |
RP35 (1953) |
RP55 (3375) |
RP75 (5262) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (12) |
Full (3744) |
Representative proteomes | UniProt (11384) |
NCBI (37745) |
Meta (1174) |
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---|---|---|---|---|---|---|---|---|---|
RP15 (598) |
RP35 (1953) |
RP55 (3375) |
RP75 (5262) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_15304 (release 7.3) & Pfam-B_5114 (Release 8.0) |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Wood V |
Number in seed: | 12 |
Number in full: | 3744 |
Average length of the domain: | 90.70 aa |
Average identity of full alignment: | 27 % |
Average coverage of the sequence by the domain: | 37.95 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 94 | ||||||||||||
Family (HMM) version: | 15 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
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Structures
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the ICMT domain has been found. There are 3 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
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