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0  structures 17  species 1  interaction 180  sequences 17  architectures

Family: Strep_his_triad (PF04270)

Summary: Streptococcal histidine triad protein

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The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Streptococcal histidine triad protein Provide feedback

All members of this family are proteins from Streptococcal species. The proteins are characterised by having a HxxHxH motif that usually occurs multiple times throughout the protein [1]. The histidine triad is predicted to bind metal cations, in particular Zn2+. The zinc is transferred, on the surface of the streptococcus from the Strep_his_triad protein, a zinc scavenger, to apo-ADCAII, a cell-surface lipoprotein transporter [3] that leads to Zn2+ uptake [2] into the bacterium.

Literature references

  1. Zhang Y, Masi AW, Barniak V, Mountzouros K, Hostetter MK, Green BA; , Infect Immun 2001;69:3827-3836.: Recombinant PhpA protein, a unique histidine motif-containing protein from Streptococcus pneumoniae, protects mice against intranasal pneumococcal challenge. PUBMED:11349048 EPMC:11349048

  2. Bersch B, Bougault C, Roux L, Favier A, Vernet T, Durmort C;, PLoS One. 2013;8:e81168.: New insights into histidine triad proteins: solution structure of a Streptococcus pneumoniae PhtD domain and zinc transfer to AdcAII. PUBMED:24312273 EPMC:24312273

  3. Loisel E, Jacquamet L, Serre L, Bauvois C, Ferrer JL, Vernet T, Di Guilmi AM, Durmort C;, J Mol Biol. 2008;381:594-606.: AdcAII, a new pneumococcal Zn-binding protein homologous with ABC transporters: biochemical and structural analysis. PUBMED:18632116 EPMC:18632116


This tab holds annotation information from the InterPro database.

InterPro entry IPR006270

This entry represents a repeated sequence region that includes a His-X-X-His-X-His (histidine triad) motif, which is found in a family of predominantly streptococcal proteins. Members of the family are suggested to cleave human complement component 3, and family member PhpA has been shown in vaccine studies to be a protective antigen in mice [PUBMED:11349048].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(26)
Full
(180)
Representative proteomes UniProt
(4270)
NCBI
(13296)
Meta
(0)
RP15
(18)
RP35
(87)
RP55
(212)
RP75
(372)
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HTML View  View               
PP/heatmap 1 View               

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(26)
Full
(180)
Representative proteomes UniProt
(4270)
NCBI
(13296)
Meta
(0)
RP15
(18)
RP35
(87)
RP55
(212)
RP75
(372)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(26)
Full
(180)
Representative proteomes UniProt
(4270)
NCBI
(13296)
Meta
(0)
RP15
(18)
RP35
(87)
RP55
(212)
RP75
(372)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download    
Gzipped Download   Download   Download   Download   Download   Download   Download   Download    

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: TIGRFAMs (release 2.0);
Previous IDs: strep_his_triad;
Type: Family
Author: TIGRFAMs, Finn RD
Number in seed: 26
Number in full: 180
Average length of the domain: 42.70 aa
Average identity of full alignment: 28 %
Average coverage of the sequence by the domain: 24.86 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 11927849 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 27.0 5.0
Trusted cut-off 31.4 5.3
Noise cut-off 25.9 4.9
Model length: 51
Family (HMM) version: 10
Download: download the raw HMM for this family

Species distribution

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Viroids Viroids Unclassified sequence Unclassified sequence

Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Interactions

There is 1 interaction for this family. More...

Strep_his_triad