Summary: Aminoglycoside/hydroxyurea antibiotic resistance kinase
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Aminoglycoside/hydroxyurea antibiotic resistance kinase Provide feedback
The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation utilising ATP. Likewise hydroxyurea is inactivated by phosphorylation of the hydroxy group in the hydroxylamine moiety [1,2,3].
Literature references
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Tohyama H, Okami Y, Umezawa H; , Nucleic Acids Res 1987;15:1819-1833.: Nucleotide sequence of the streptomycinphosphotransferase and amidinotransferase genes from Streptomyces griseus. PUBMED:3029728 EPMC:3029728
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Davies J, Wright GD; , Trends Microbiol 1997;5:234-240.: Bacterial resistance to aminoglycoside antibiotics. PUBMED:9211644 EPMC:9211644
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Kormanec J, Farkasovsky M, Potuckova L, Godar S; , Gene 1992;114:133-137.: A gene (hur) from Streptomyces aureofaciens, conferring resistance to hydroxyurea, is related to genes encoding streptomycin phosphotransferase. PUBMED:1316866 EPMC:1316866
Internal database links
SCOOP: | APH Choline_kinase EcKinase Fructosamin_kin |
Similarity to PfamA using HHSearch: | APH Fructosamin_kin |
This tab holds annotation information from the InterPro database.
InterPro entry IPR006748
The aminoglycosides are a large group of biologically active bacterial secondary metabolites, best known for their antibiotic properties [PUBMED:9211644]. Aminoglycoside phosphotransferases achieve inactivation of these enzymes by phosphorylation, utilising ATP. Likewise, hydroxyurea is inactivated by phosphorylation of the hydroxy group in the hydroxylamine moiety.
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Molecular function | phosphotransferase activity, alcohol group as acceptor (GO:0016773) |
Biological process | protein phosphorylation (GO:0006468) |
secondary metabolic process (GO:0019748) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan PKinase (CL0016), which has the following description:
This superfamily includes the Serine/Threonine- and Tyrosine- protein kinases as well as related kinases that act on non-protein substrates.
The clan contains the following 38 members:
ABC1 AceK Act-Frag_cataly Alpha_kinase APH APH_6_hur Choline_kinase CotH DUF1679 DUF2252 DUF4135 EcKinase Fam20C Fructosamin_kin FTA2 Haspin_kinase HipA_C Ins_P5_2-kin IPK IucA_IucC Kdo Kinase-like Kinase-PolyVal KIND Pan3_PK PI3_PI4_kinase PIP49_C PIP5K PK_Tyr_Ser-Thr Pkinase Pkinase_fungal Pox_ser-thr_kin RIO1 Seadorna_VP7 UL97 WaaY YrbL-PhoP_reg YukCAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the UniProtKB sequence database, the NCBI sequence database, and our metagenomics sequence database. More...
View options
We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (4) |
Full (1494) |
Representative proteomes | UniProt (6624) |
NCBI (12246) |
Meta (30) |
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RP15 (61) |
RP35 (551) |
RP55 (1592) |
RP75 (2932) |
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PP/heatmap | 1 |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key:
available,
not generated,
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (4) |
Full (1494) |
Representative proteomes | UniProt (6624) |
NCBI (12246) |
Meta (30) |
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RP15 (61) |
RP35 (551) |
RP55 (1592) |
RP75 (2932) |
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Raw Stockholm | |||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
Trees
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Curation
Seed source: | Pfam-B_4369 (release 7.5) |
Previous IDs: | none |
Type: | Family |
Sequence Ontology: | SO:0100021 |
Author: |
Waterfield DI |
Number in seed: | 4 |
Number in full: | 1494 |
Average length of the domain: | 236.70 aa |
Average identity of full alignment: | 28 % |
Average coverage of the sequence by the domain: | 81.16 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq
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Model details: |
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Model length: | 252 | ||||||||||||
Family (HMM) version: | 15 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
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