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5  structures 751  species 0  interactions 941  sequences 14  architectures

Family: TAFII55_N (PF04658)

Summary: TAFII55 protein conserved region

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This is the Wikipedia entry entitled "TAF7". More...

TAF7 Edit Wikipedia article

TAF7
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases TAF7, TAF2F, TAFII55, TATA-box binding protein associated factor 7
External IDs MGI: 1346348 HomoloGene: 133942 GeneCards: TAF7
Gene location (Human)
Chromosome 5 (human)
Chr. Chromosome 5 (human)[1]
Chromosome 5 (human)
Genomic location for TAF7
Genomic location for TAF7
Band 5q31.3 Start 141,260,225 bp[1]
End 141,320,821 bp[1]
RNA expression pattern
PBB GE TAF7 201023 at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_005642

NM_175770

RefSeq (protein)

NP_005633

NP_786964

Location (UCSC) Chr 5: 141.26 – 141.32 Mb Chr 5: 37.64 – 37.64 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Transcription initiation factor TFIID subunit 7 also known as TAFII55 is a protein that in humans is encoded by the TAF7 gene.[5]

Function

The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II.[6]

TAFII55_N
Identifiers
Symbol TAFII55_N
Pfam PF04658
InterPro IPR006751

The general transcription factor, TFIID, consists of the TATA-binding protein (TBP) associated with a series of TBP-associated factors (TAFs) that together participate in the assembly of the transcription preinitiation complex. TAFII55 binds to TAFII250 and inhibits its acetyltransferase activity. The exact role of TAFII55 is currently unknown but studies have shown that it interacts with the C-jun pathway.[7] The conserved region is situated towards the N-terminal of the protein.[8] This entry talks about the N-terminal domain.

Interestingly, crystallographic studies have revealed a very significant hydrophobic pocket between TAF7 and TAF1, its main binding partner. Due to the incredible hydrophobicity of this interaction, its unlikley that TAF1 would be able to fold properly without the presence of TAF7. Thus, it is possible that TAF7 is required for proper production of TAF1[9]

Interactions

TAF7 has been shown to interact with:

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000178913 - Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000051316 - Ensembl, May 2017
  3. ^ "Human PubMed Reference:". 
  4. ^ "Mouse PubMed Reference:". 
  5. ^ Chiang CM, Roeder RG (January 1995). "Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators". Science. 267 (5197): 531–6. PMID 7824954. doi:10.1126/science.7824954. 
  6. ^ "Entrez Gene: TAF7 TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa". 
  7. ^ Munz C, Psichari E, Mandilis D, Lavigne AC, Spiliotaki M, Oehler T, Davidson I, Tora L, Angel P, Pintzas A (June 2003). "TAF7 (TAFII55) plays a role in the transcription activation by c-Jun". The Journal of Biological Chemistry. 278 (24): 21510–6. PMID 12676957. doi:10.1074/jbc.M212764200. 
  8. ^ a b Gegonne A, Weissman JD, Singer DS (October 2001). "TAFII55 binding to TAFII250 inhibits its acetyltransferase activity". Proceedings of the National Academy of Sciences of the United States of America. 98 (22): 12432–7. PMC 60071Freely accessible. PMID 11592977. doi:10.1073/pnas.211444798. 
  9. ^ Bhattacharya S, Lou X, Hwang P, Rajashankar KR, Wang X, Gustafsson JÅ, Fletterick RJ, Jacobson RH, Webb P (June 2014). "Structural and functional insight into TAF1-TAF7, a subcomplex of transcription factor II D". Proceedings of the National Academy of Sciences of the United States of America. 111 (25): 9103–8. PMID 24927529. doi:10.1073/pnas.1408293111. 
  10. ^ Bertolotti A, Melot T, Acker J, Vigneron M, Delattre O, Tora L (March 1998). "EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". Molecular and Cellular Biology. 18 (3): 1489–97. PMC 108863Freely accessible. PMID 9488465. 
  11. ^ Pointud JC, Mengus G, Brancorsini S, Monaco L, Parvinen M, Sassone-Corsi P, Davidson I (May 2003). "The intracellular localisation of TAF7L, a paralogue of transcription factor TFIID subunit TAF7, is developmentally regulated during male germ-cell differentiation". Journal of Cell Science. 116 (Pt 9): 1847–58. PMID 12665565. doi:10.1242/jcs.00391. 
  12. ^ Bellorini M, Lee DK, Dantonel JC, Zemzoumi K, Roeder RG, Tora L, Mantovani R (June 1997). "CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues". Nucleic Acids Research. 25 (11): 2174–81. PMC 146709Freely accessible. PMID 9153318. doi:10.1093/nar/25.11.2174. 

Further reading

External links

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

TAFII55 protein conserved region Provide feedback

The general transcription factor, TFIID, consists of the TATA-binding protein (TBP) associated with a series of TBP-associated factors (TAFs) that together participate in the assembly of the transcription preinitiation complex. TAFII55 binds to TAFII250 and inhibits it acetyltransferase activity. The exact role of TAFII55 is currently unknown. The conserved region is situated towards the N-terminus of the protein [1].

Literature references

  1. Gegonne A, Weissman JD, Singer DS; , Proc Natl Acad Sci U S A 2001;98:12432-12437.: TAFII55 binding to TAFII250 inhibits its acetyltransferase activity. PUBMED:11592977 EPMC:11592977


This tab holds annotation information from the InterPro database.

InterPro entry IPR006751

The general transcription factor, TFIID, consists of the TATA-binding protein (TBP) associated with a series of TBP-associated factors (TAFs) that together participate in the assembly of the transcription preinitiation complex. TAFII55 binds to TAFII250 and inhibits its acetyltransferase activity. The exact role of TAFII55 is currently unknown. The conserved region is situated towards the N-terminal of the protein [PUBMED:11592977].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

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Alignments

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  Seed
(138)
Full
(941)
Representative proteomes UniProt
(1353)
NCBI
(1663)
Meta
(1)
RP15
(244)
RP35
(504)
RP55
(743)
RP75
(929)
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  Seed
(138)
Full
(941)
Representative proteomes UniProt
(1353)
NCBI
(1663)
Meta
(1)
RP15
(244)
RP35
(504)
RP55
(743)
RP75
(929)
Alignment:
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  Seed
(138)
Full
(941)
Representative proteomes UniProt
(1353)
NCBI
(1663)
Meta
(1)
RP15
(244)
RP35
(504)
RP55
(743)
RP75
(929)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download   Download   Download  
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You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

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Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

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Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_4395 (release 7.5)
Previous IDs: none
Type: Family
Author: Waterfield DI, Finn RD
Number in seed: 138
Number in full: 941
Average length of the domain: 151.90 aa
Average identity of full alignment: 35 %
Average coverage of the sequence by the domain: 37.06 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.0 25.0
Trusted cut-off 25.5 26.0
Noise cut-off 23.9 24.7
Model length: 160
Family (HMM) version: 12
Download: download the raw HMM for this family

Species distribution

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the TAFII55_N domain has been found. There are 5 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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